Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs

Detalhes bibliográficos
Autor(a) principal: Costa, P. F.
Data de Publicação: 2015
Outros Autores: Hutmacher, D. W., Theodoropoulos, Christina, Gomes, Manuela E., Reis, R. L., Vaquette, C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/36605
Resumo: The ability to test large arrays of cell and biomaterial combinations in 3D environments is still rather limited in the context of tissue engineering and regenerative medicine. This limitation can be generally addressed by employing highly automated and reproducible methodologies. This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface-coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP-coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.
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spelling Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructsAdditive manufacturingBioreactorsRegenerative medicineTissue engineeringScience & TechnologyThe ability to test large arrays of cell and biomaterial combinations in 3D environments is still rather limited in the context of tissue engineering and regenerative medicine. This limitation can be generally addressed by employing highly automated and reproducible methodologies. This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface-coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP-coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.P.F.C. and D.W.H. contributed equally to this work. This work was supported by the Australian Research Council. P.F.C. acknowledges the Portuguese Foundation for Science and Technology for his PhD grant (SFRH/BD/62452/2009).Wiley-VCH VerlagUniversidade do MinhoCosta, P. F.Hutmacher, D. W.Theodoropoulos, ChristinaGomes, Manuela E.Reis, R. L.Vaquette, C.2015-042015-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/36605engCosta P. F., Hutmacher D. W., Theodoropoulos C., Gomes M. E., Reis R. L., Vaquette C. Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs, Advanced Healthcare Materials, Vol. 4, Issue 6, pp. 864-873, doi:10.1002/adhm.201400591, 20152192-265910.1002/adhm.20140059125721231info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:42:09Zoai:repositorium.sdum.uminho.pt:1822/36605Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:39:20.580158Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
title Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
spellingShingle Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
Costa, P. F.
Additive manufacturing
Bioreactors
Regenerative medicine
Tissue engineering
Science & Technology
title_short Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
title_full Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
title_fullStr Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
title_full_unstemmed Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
title_sort Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs
author Costa, P. F.
author_facet Costa, P. F.
Hutmacher, D. W.
Theodoropoulos, Christina
Gomes, Manuela E.
Reis, R. L.
Vaquette, C.
author_role author
author2 Hutmacher, D. W.
Theodoropoulos, Christina
Gomes, Manuela E.
Reis, R. L.
Vaquette, C.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Costa, P. F.
Hutmacher, D. W.
Theodoropoulos, Christina
Gomes, Manuela E.
Reis, R. L.
Vaquette, C.
dc.subject.por.fl_str_mv Additive manufacturing
Bioreactors
Regenerative medicine
Tissue engineering
Science & Technology
topic Additive manufacturing
Bioreactors
Regenerative medicine
Tissue engineering
Science & Technology
description The ability to test large arrays of cell and biomaterial combinations in 3D environments is still rather limited in the context of tissue engineering and regenerative medicine. This limitation can be generally addressed by employing highly automated and reproducible methodologies. This study reports on the development of a highly versatile and upscalable method based on additive manufacturing for the fabrication of arrays of scaffolds, which are enclosed into individualized perfusion chambers. Devices containing eight scaffolds and their corresponding bioreactor chambers are simultaneously fabricated utilizing a dual extrusion additive manufacturing system. To demonstrate the versatility of the concept, the scaffolds, while enclosed into the device, are subsequently surface-coated with a biomimetic calcium phosphate layer by perfusion with simulated body fluid solution. 96 scaffolds are simultaneously seeded and cultured with human osteoblasts under highly controlled bidirectional perfusion dynamic conditions over 4 weeks. Both coated and noncoated resulting scaffolds show homogeneous cell distribution and high cell viability throughout the 4 weeks culture period and CaP-coated scaffolds result in a significantly increased cell number. The methodology developed in this work exemplifies the applicability of additive manufacturing as a tool for further automation of studies in the field of tissue engineering and regenerative medicine.
publishDate 2015
dc.date.none.fl_str_mv 2015-04
2015-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/36605
url http://hdl.handle.net/1822/36605
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Costa P. F., Hutmacher D. W., Theodoropoulos C., Gomes M. E., Reis R. L., Vaquette C. Additively manufactured device for dynamic culture of large arrays of 3D tissue engineered constructs, Advanced Healthcare Materials, Vol. 4, Issue 6, pp. 864-873, doi:10.1002/adhm.201400591, 2015
2192-2659
10.1002/adhm.201400591
25721231
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-VCH Verlag
publisher.none.fl_str_mv Wiley-VCH Verlag
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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