The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells

Detalhes bibliográficos
Autor(a) principal: Henriques, Mariana
Data de Publicação: 2007
Outros Autores: Azeredo, Joana, Oliveira, Rosário
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/6942
Resumo: Candida albicans and Candida dubliniensis are two pathogenic yeasts particularly hazardous to immunocompromised patients. Adhesion of yeast cells to epithelium is considered one of the virulence factors and its study is of major importance. The main aim of this study was the comparison of the influence of physico-chemical properties on the adhesion of C. albicans and C. dubliniensis to epithelium. Two strains of each Candida species were used in the adhesion assays to HeLa cells. Adhered cells were enumerated by direct microscopic images observation. Yeast cell surface tension parameters and degree of hydrophobicity were determined by contact angle measurement. Pseudohyphae and hyphae formation was analysed by scanning electron microscopy. Yeast cells presented no statistical differences concerning their physico-chemical surface properties. However, the extent of adhesion to epithelium was different among the four strains. As general conclusion, yeast adhesion to epithelium seems to be strain-dependent and not directly correlated with pseudohyphae formation.
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spelling The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cellsCandida albicansCandida dubliniensisadhesionepithelial cellsScience & TechnologyCandida albicans and Candida dubliniensis are two pathogenic yeasts particularly hazardous to immunocompromised patients. Adhesion of yeast cells to epithelium is considered one of the virulence factors and its study is of major importance. The main aim of this study was the comparison of the influence of physico-chemical properties on the adhesion of C. albicans and C. dubliniensis to epithelium. Two strains of each Candida species were used in the adhesion assays to HeLa cells. Adhered cells were enumerated by direct microscopic images observation. Yeast cell surface tension parameters and degree of hydrophobicity were determined by contact angle measurement. Pseudohyphae and hyphae formation was analysed by scanning electron microscopy. Yeast cells presented no statistical differences concerning their physico-chemical surface properties. However, the extent of adhesion to epithelium was different among the four strains. As general conclusion, yeast adhesion to epithelium seems to be strain-dependent and not directly correlated with pseudohyphae formation.Fundação para a Ciência e a Tecnologia (FCT); Department of Biology, University of Minho; Instituto de Ciêncicas Biomédicas Abel Salazar, University of Porto; Faculty of Pharmacy, University of Lisbon, Portugal.Blackwell Publishing LtdUniversidade do MinhoHenriques, MarianaAzeredo, JoanaOliveira, Rosário2007-092007-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/6942eng"Mycoses". ISSN 0933-7407. 50:5 (Sept. 2007) 391-396.0933-740710.1111/j.1439-0507.2007.01387.x17714359info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T05:18:45Zoai:repositorium.sdum.uminho.pt:1822/6942Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T05:18:45Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
title The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
spellingShingle The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
Henriques, Mariana
Candida albicans
Candida dubliniensis
adhesion
epithelial cells
Science & Technology
title_short The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
title_full The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
title_fullStr The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
title_full_unstemmed The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
title_sort The involvement of physico-chemical interactions in the adhesion of Candida albicans and Candida dubliniensis to epithelial cells
author Henriques, Mariana
author_facet Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
author_role author
author2 Azeredo, Joana
Oliveira, Rosário
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Henriques, Mariana
Azeredo, Joana
Oliveira, Rosário
dc.subject.por.fl_str_mv Candida albicans
Candida dubliniensis
adhesion
epithelial cells
Science & Technology
topic Candida albicans
Candida dubliniensis
adhesion
epithelial cells
Science & Technology
description Candida albicans and Candida dubliniensis are two pathogenic yeasts particularly hazardous to immunocompromised patients. Adhesion of yeast cells to epithelium is considered one of the virulence factors and its study is of major importance. The main aim of this study was the comparison of the influence of physico-chemical properties on the adhesion of C. albicans and C. dubliniensis to epithelium. Two strains of each Candida species were used in the adhesion assays to HeLa cells. Adhered cells were enumerated by direct microscopic images observation. Yeast cell surface tension parameters and degree of hydrophobicity were determined by contact angle measurement. Pseudohyphae and hyphae formation was analysed by scanning electron microscopy. Yeast cells presented no statistical differences concerning their physico-chemical surface properties. However, the extent of adhesion to epithelium was different among the four strains. As general conclusion, yeast adhesion to epithelium seems to be strain-dependent and not directly correlated with pseudohyphae formation.
publishDate 2007
dc.date.none.fl_str_mv 2007-09
2007-09-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/6942
url http://hdl.handle.net/1822/6942
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Mycoses". ISSN 0933-7407. 50:5 (Sept. 2007) 391-396.
0933-7407
10.1111/j.1439-0507.2007.01387.x
17714359
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing Ltd
publisher.none.fl_str_mv Blackwell Publishing Ltd
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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