Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Célia Fortuna
Data de Publicação: 2018
Outros Autores: Rodrigues, M. Elisa., Henriques, Mariana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/55253
Resumo: Candidiases are the most recurrent fungal infections, especially among immunosuppressed patients. Although Candida albicans is still the most widespread isolated species, non-Candida albicans Candida species have been increasing. The goal of this work was to determine the susceptibility of C. glabrata biofilms to echinocandins and to evaluate their effect on the biofilm matrix composition, comparing the results with other Candida species. Drug susceptibilities were assessed through the determination of minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and minimum biofilm eradication concentration (MBEC) of caspofungin (Csf) and micafugin (Mcf). The -1,3 glucans content of the matrices was assessed after contact with the drugs. The data suggest that, generally, after contact with echinocandins, the concentration of -1,3 glucans increased. These adjustments in the matrix composition of C. glabrata biofilms and the chemical differences between Csf and Mcf, seem responsible and may determine the effectivity of the drug responses.
id RCAP_9f5aad3a971c7747d7bb78dae07a889e
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/55253
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix compositionCandida glabrataechinocandinbiofilmmatrixantifungal drugresistanceScience & TechnologyCandidiases are the most recurrent fungal infections, especially among immunosuppressed patients. Although Candida albicans is still the most widespread isolated species, non-Candida albicans Candida species have been increasing. The goal of this work was to determine the susceptibility of C. glabrata biofilms to echinocandins and to evaluate their effect on the biofilm matrix composition, comparing the results with other Candida species. Drug susceptibilities were assessed through the determination of minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and minimum biofilm eradication concentration (MBEC) of caspofungin (Csf) and micafugin (Mcf). The -1,3 glucans content of the matrices was assessed after contact with the drugs. The data suggest that, generally, after contact with echinocandins, the concentration of -1,3 glucans increased. These adjustments in the matrix composition of C. glabrata biofilms and the chemical differences between Csf and Mcf, seem responsible and may determine the effectivity of the drug responses.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 [POCI-01–0145-FEDER-006684] and BioTecNorte operation [NORTE-01–0145-FEDER-000004] funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte, Célia F. Rodrigues’ [SFRH/BD/93078/2013] PhD grant and M. Elisa Rodrigues [SFRH/BPD/95401/2013] post-doctoral grant.info:eu-repo/semantics/publishedVersionTaylor and FrancisUniversidade do MinhoRodrigues, Célia FortunaRodrigues, M. Elisa.Henriques, Mariana20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/55253engRodrigues, Célia F.; Rodrigues, M. Elisa; Henriques, Mariana, Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition. Biofouling - The Journal of Bioadhesion and Biofilm Research, 34(5), 569-578, 20180892-70141029-245410.1080/08927014.2018.147224429798695http://www.tandfonline.com/action/journalInformation?journalCode=gbif20info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:30:46Zoai:repositorium.sdum.uminho.pt:1822/55253Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:26:00.811938Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
title Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
spellingShingle Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
Rodrigues, Célia Fortuna
Candida glabrata
echinocandin
biofilm
matrix
antifungal drug
resistance
Science & Technology
title_short Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
title_full Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
title_fullStr Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
title_full_unstemmed Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
title_sort Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition
author Rodrigues, Célia Fortuna
author_facet Rodrigues, Célia Fortuna
Rodrigues, M. Elisa.
Henriques, Mariana
author_role author
author2 Rodrigues, M. Elisa.
Henriques, Mariana
author2_role author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Rodrigues, Célia Fortuna
Rodrigues, M. Elisa.
Henriques, Mariana
dc.subject.por.fl_str_mv Candida glabrata
echinocandin
biofilm
matrix
antifungal drug
resistance
Science & Technology
topic Candida glabrata
echinocandin
biofilm
matrix
antifungal drug
resistance
Science & Technology
description Candidiases are the most recurrent fungal infections, especially among immunosuppressed patients. Although Candida albicans is still the most widespread isolated species, non-Candida albicans Candida species have been increasing. The goal of this work was to determine the susceptibility of C. glabrata biofilms to echinocandins and to evaluate their effect on the biofilm matrix composition, comparing the results with other Candida species. Drug susceptibilities were assessed through the determination of minimum inhibitory concentration (MIC), minimum fungicidal concentration (MFC) and minimum biofilm eradication concentration (MBEC) of caspofungin (Csf) and micafugin (Mcf). The -1,3 glucans content of the matrices was assessed after contact with the drugs. The data suggest that, generally, after contact with echinocandins, the concentration of -1,3 glucans increased. These adjustments in the matrix composition of C. glabrata biofilms and the chemical differences between Csf and Mcf, seem responsible and may determine the effectivity of the drug responses.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/55253
url http://hdl.handle.net/1822/55253
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Rodrigues, Célia F.; Rodrigues, M. Elisa; Henriques, Mariana, Susceptibility of Candida glabrata biofilms to echinocandins: alterations in the matrix composition. Biofouling - The Journal of Bioadhesion and Biofilm Research, 34(5), 569-578, 2018
0892-7014
1029-2454
10.1080/08927014.2018.1472244
29798695
http://www.tandfonline.com/action/journalInformation?journalCode=gbif20
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132746459643904