Combined detection of nitrite and bioelectrical activity using microelectrode arrays and a phosphate buffered saline solution

• Autor(a) principal: Almeida, Fernando Luis de
• Data de Publicação: 2021
• Outros Autores: Souza Junior, Wilmar, Moraes, Yann Lima, dos Santos Filho, Sebastião Gomes, Veronez, Douglas, Cestari, Idagene
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: https://doi.org/10.34629/ipl.isel.i-ETC.73
• topic: Microelectronics; Electrochemical; Electronics; Bioengineering; extracelullar local field potentials, myoblasts, gold microelectrodes, nitrite, heart.

This paper presents the manufacturing of amicroelectrode array, on printed circuit boards (PCB) and onsilicon substrate, composed of 60 to 64 gold microelectrodes(between 4 µm and 70 µm each in diameter). The sensor has ameasurement total area of 0.5 cm in radius, one referenceelectrode (1 mm2 in area) and 28.4 mm wide and 28.4 mm long.These microelectrodes were used for checking and logging ofextracelullar local field potential of cell culture and nitritemeasurement in a phosphate buffered saline solution(electrolytical aqueous medium). In addition, an apparatus toshield from electromagnetic interference for connecting thearrays was designed to allow the capture of electrochemicalreactions or electronic signals by the microelectrodes, forexample: nitrite or cardiac potential measurement, respectively.Finally, biocompatibility tests of the array structures wereperformed. The preliminary electrical and biocompatibilitytesting, along with the collected data, has shown promisingresults pointing to the development of an accurate sensor afterthe completion of this study. The sensor has potentially abroader range of applications with only a few adaptations anddue its good accuracy it can be a very useful resource for manychemical and biological applications.