Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.6/4625 |
Resumo: | Candida spp. are common causative agents of mucocutaneous infections. New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols. Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties. The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts. CH antifungal activity was assessed according to CLSI reference M27-A3 protocol; its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay. CH demonstrated a full inhibition of C. tropicalis, C. krusei, C. guilliermondii and C. parapsilosis growth while impairing C. albicans and C. glabrata viability. Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane. However, CH showed no cytotoxic effect upon human fibroblasts cells. Resistant strains will require new therapeutic approaches. Chitosan being a good carrier and having itself anti-Candida activity seems to be a promising vehicle to be used for the treatment of mucocutaneous candidosis. |
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Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profileChitosan hydrogelAnticandida activityBiodegradableCandida spp. are common causative agents of mucocutaneous infections. New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols. Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties. The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts. CH antifungal activity was assessed according to CLSI reference M27-A3 protocol; its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay. CH demonstrated a full inhibition of C. tropicalis, C. krusei, C. guilliermondii and C. parapsilosis growth while impairing C. albicans and C. glabrata viability. Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane. However, CH showed no cytotoxic effect upon human fibroblasts cells. Resistant strains will require new therapeutic approaches. Chitosan being a good carrier and having itself anti-Candida activity seems to be a promising vehicle to be used for the treatment of mucocutaneous candidosis.KARGERuBibliorumOliveira, Ana Palmeira deRibeiro, MP.Oliveira, Rita Manuela Palmeira deGaspar, CarlosOliveira, Sofia Costa deCorreia, I.J.Vaz, Cidália PinaOliveira, José António Martinez Souto deQueiroz, JoãoRodrigues, Acácio2018-03-16T14:17:16Z2010-10-162010-10-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.6/4625engPalmeira-de-Oliveira, A., Ribeiro, M.P., Palmeira-de-Oliveira, R., Gaspar, C., Costa-de-Oliveira, S., Correia, I.J., Queiroz, J.A., Pina Vaz C., Martinez-de-Oliveira, J. e Rodrigues, A.G. (2010) “Anticandida Activity of a Chitosan Hydrogel: Mechanism of Action and Cytotoxicity Profile”, Gynecologic and Obstetric Investigation Journal, Vol. 70(4), pp. 322-32710.1159/000314023metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-15T09:41:42Zoai:ubibliorum.ubi.pt:10400.6/4625Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:45:41.321991Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
title |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
spellingShingle |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile Oliveira, Ana Palmeira de Chitosan hydrogel Anticandida activity Biodegradable |
title_short |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
title_full |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
title_fullStr |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
title_full_unstemmed |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
title_sort |
Anti-Candida activity of a chitosan hydrogel: mechanism of action and cytotoxicity profile |
author |
Oliveira, Ana Palmeira de |
author_facet |
Oliveira, Ana Palmeira de Ribeiro, MP. Oliveira, Rita Manuela Palmeira de Gaspar, Carlos Oliveira, Sofia Costa de Correia, I.J. Vaz, Cidália Pina Oliveira, José António Martinez Souto de Queiroz, João Rodrigues, Acácio |
author_role |
author |
author2 |
Ribeiro, MP. Oliveira, Rita Manuela Palmeira de Gaspar, Carlos Oliveira, Sofia Costa de Correia, I.J. Vaz, Cidália Pina Oliveira, José António Martinez Souto de Queiroz, João Rodrigues, Acácio |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
uBibliorum |
dc.contributor.author.fl_str_mv |
Oliveira, Ana Palmeira de Ribeiro, MP. Oliveira, Rita Manuela Palmeira de Gaspar, Carlos Oliveira, Sofia Costa de Correia, I.J. Vaz, Cidália Pina Oliveira, José António Martinez Souto de Queiroz, João Rodrigues, Acácio |
dc.subject.por.fl_str_mv |
Chitosan hydrogel Anticandida activity Biodegradable |
topic |
Chitosan hydrogel Anticandida activity Biodegradable |
description |
Candida spp. are common causative agents of mucocutaneous infections. New therapeutic antifungal drugs are needed to treat chronic disease as these are frequently clinically resistant to azols. Chitosan, among other possible vehicles for active compounds, shows an added value as it appears to have intrinsic antimicrobial properties. The aim of the present study was to evaluate the anti-Candida activity of a medium-molecular-weight chitosan hydrogel (CH), to clarify its possible mechanism of action and to evaluate its cytotoxicity on human fibroblasts. CH antifungal activity was assessed according to CLSI reference M27-A3 protocol; its mechanism of action was investigated by flow cytometry, and its cytotoxicity was studied by MTT assay. CH demonstrated a full inhibition of C. tropicalis, C. krusei, C. guilliermondii and C. parapsilosis growth while impairing C. albicans and C. glabrata viability. Flow cytometry tests showed that CH acts by inducing primary lesion of the cytoplasmic membrane. However, CH showed no cytotoxic effect upon human fibroblasts cells. Resistant strains will require new therapeutic approaches. Chitosan being a good carrier and having itself anti-Candida activity seems to be a promising vehicle to be used for the treatment of mucocutaneous candidosis. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-16 2010-10-16T00:00:00Z 2018-03-16T14:17:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.6/4625 |
url |
http://hdl.handle.net/10400.6/4625 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Palmeira-de-Oliveira, A., Ribeiro, M.P., Palmeira-de-Oliveira, R., Gaspar, C., Costa-de-Oliveira, S., Correia, I.J., Queiroz, J.A., Pina Vaz C., Martinez-de-Oliveira, J. e Rodrigues, A.G. (2010) “Anticandida Activity of a Chitosan Hydrogel: Mechanism of Action and Cytotoxicity Profile”, Gynecologic and Obstetric Investigation Journal, Vol. 70(4), pp. 322-327 10.1159/000314023 |
dc.rights.driver.fl_str_mv |
metadata only access info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
metadata only access |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
KARGER |
publisher.none.fl_str_mv |
KARGER |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799136354405187584 |