DNA metabarcoding methods for the study of marine benthic meiofauna: A review

Detalhes bibliográficos
Autor(a) principal: Gielings, Romy
Data de Publicação: 2021
Outros Autores: Fais, Maria, Fontaneto, Diego, Creer, Simon, Costa, Filipe O., Renema, Willem, Macher, Jan-Niklas
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/1822/79968
Resumo: The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmars 2021.730063/full#supplementary-material
id RCAP_a06193643c3c19a12f0273db67798ff3
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/79968
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling DNA metabarcoding methods for the study of marine benthic meiofauna: A reviewMeiofaunaDNA metabarcodingMarine ecosystemBiomonitoringMeiobenthosMolecular methodsSampling techniquesScience & TechnologyThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmars 2021.730063/full#supplementary-materialMeiofaunal animals, roughly between 0.045 and 1 mm in size, are ubiquitous and ecologically important inhabitants of benthic marine ecosystems. Their high species richness and rapid response to environmental change make them promising targets for ecological and biomonitoring studies. However, diversity patterns of benthic marine meiofauna remain poorly known due to challenges in species identification using classical morphological methods. DNA metabarcoding is a powerful tool to overcome this limitation. Here, we review DNA metabarcoding approaches used in studies on marine meiobenthos with the aim of facilitating researchers to make informed decisions for the implementation of DNA metabarcoding in meiofaunal biodiversity monitoring. We found that the applied methods vary greatly between researchers and studies, and concluded that further explicit comparisons of protocols are needed to apply DNA metabarcoding as a standard tool for assessing benthic meiofaunal community composition. Key aspects that require additional consideration include: (1) comparability of sample pre-treatment methods; (2) integration of different primers and molecular markers for both the mitochondrial cytochrome c oxidase subunit I (COI) and the nuclear 18S rRNA genes to maximize taxon recovery; (3) precise and standardized description of sampling methods to allow for comparison and replication; and (4) evaluation and testing of bioinformatic pipelines to enhance comparability between studies. By enhancing comparability between the various approaches currently used for the different aspects of the analyses, DNA metabarcoding will improve the long-term integrative potential for surveying and biomonitoring marine benthic meiofauna.This work was funded by a BEN (Biodiversity-Ecology-Nature) grant (Number T0206/37197/2021/kg) of the Bauer-Hollmann foundation to J-NM.Frontiers MediaUniversidade do MinhoGielings, RomyFais, MariaFontaneto, DiegoCreer, SimonCosta, Filipe O.Renema, WillemMacher, Jan-Niklas20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/1822/79968engGielings, R., Fais, M., Fontaneto, D., Creer, S., Costa, F. O., Renema, W., & Macher, J.-N. (2021). DNA Metabarcoding Methods for the Study of Marine Benthic Meiofauna: A Review. Frontiers in Marine Science, 8. doi: 10.3389/fmars.2021.7300632296-774510.3389/fmars.2021.730063https://www.frontiersin.org/articles/10.3389/fmars.2021.730063info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:28:07Zoai:repositorium.sdum.uminho.pt:1822/79968Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:52.002167Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv DNA metabarcoding methods for the study of marine benthic meiofauna: A review
title DNA metabarcoding methods for the study of marine benthic meiofauna: A review
spellingShingle DNA metabarcoding methods for the study of marine benthic meiofauna: A review
Gielings, Romy
Meiofauna
DNA metabarcoding
Marine ecosystem
Biomonitoring
Meiobenthos
Molecular methods
Sampling techniques
Science & Technology
title_short DNA metabarcoding methods for the study of marine benthic meiofauna: A review
title_full DNA metabarcoding methods for the study of marine benthic meiofauna: A review
title_fullStr DNA metabarcoding methods for the study of marine benthic meiofauna: A review
title_full_unstemmed DNA metabarcoding methods for the study of marine benthic meiofauna: A review
title_sort DNA metabarcoding methods for the study of marine benthic meiofauna: A review
author Gielings, Romy
author_facet Gielings, Romy
Fais, Maria
Fontaneto, Diego
Creer, Simon
Costa, Filipe O.
Renema, Willem
Macher, Jan-Niklas
author_role author
author2 Fais, Maria
Fontaneto, Diego
Creer, Simon
Costa, Filipe O.
Renema, Willem
Macher, Jan-Niklas
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gielings, Romy
Fais, Maria
Fontaneto, Diego
Creer, Simon
Costa, Filipe O.
Renema, Willem
Macher, Jan-Niklas
dc.subject.por.fl_str_mv Meiofauna
DNA metabarcoding
Marine ecosystem
Biomonitoring
Meiobenthos
Molecular methods
Sampling techniques
Science & Technology
topic Meiofauna
DNA metabarcoding
Marine ecosystem
Biomonitoring
Meiobenthos
Molecular methods
Sampling techniques
Science & Technology
description The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmars 2021.730063/full#supplementary-material
publishDate 2021
dc.date.none.fl_str_mv 2021
2021-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/1822/79968
url https://hdl.handle.net/1822/79968
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Gielings, R., Fais, M., Fontaneto, D., Creer, S., Costa, F. O., Renema, W., & Macher, J.-N. (2021). DNA Metabarcoding Methods for the Study of Marine Benthic Meiofauna: A Review. Frontiers in Marine Science, 8. doi: 10.3389/fmars.2021.730063
2296-7745
10.3389/fmars.2021.730063
https://www.frontiersin.org/articles/10.3389/fmars.2021.730063
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132700937814016

Registros relacionados