DNA−Surfactant Complexes at Solid Surfaces
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10625 https://doi.org/10.1021/la000993e |
Resumo: | In this work, we report on the adsorption of complexes between DNA of different molecular weight and a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on hydrophobized and hydrophilic negatively charged silica surfaces as measured by ellipsometry. We will demonstrate how the adsorption is affected by the state of the DNA−surfactant complexes formed in bulk solution. High molecular weight DNA molecules, which condense (transform from coil to globule state) on addition of small amounts of cationic surfactants, do not adsorb on hydrophilic silica prior to phase separation. However, DNA−surfactant complexes formed from low molecular weight DNA were found to adsorb. For these complexes surfactants interact with DNA, without condensation of the DNA. Adsorbed DNA−surfactant complexes can easily be removed from the hydrophilic silica surface when replacing the bulk DNA−surfactant solution with pure salt solution. At the hydrophobic surface the DNA adsorbs without addition of cationic surfactant. However, with addition of a very low amount of surfactant, a rapid increase in adsorbed amount and a simultaneous decrease in adsorbed layer thickness are observed. This compaction of the adsorbed layer is to some extent reversible when replacing the bulk DNA−surfactant solution with pure salt solution. |
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DNA−Surfactant Complexes at Solid SurfacesIn this work, we report on the adsorption of complexes between DNA of different molecular weight and a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on hydrophobized and hydrophilic negatively charged silica surfaces as measured by ellipsometry. We will demonstrate how the adsorption is affected by the state of the DNA−surfactant complexes formed in bulk solution. High molecular weight DNA molecules, which condense (transform from coil to globule state) on addition of small amounts of cationic surfactants, do not adsorb on hydrophilic silica prior to phase separation. However, DNA−surfactant complexes formed from low molecular weight DNA were found to adsorb. For these complexes surfactants interact with DNA, without condensation of the DNA. Adsorbed DNA−surfactant complexes can easily be removed from the hydrophilic silica surface when replacing the bulk DNA−surfactant solution with pure salt solution. At the hydrophobic surface the DNA adsorbs without addition of cationic surfactant. However, with addition of a very low amount of surfactant, a rapid increase in adsorbed amount and a simultaneous decrease in adsorbed layer thickness are observed. This compaction of the adsorbed layer is to some extent reversible when replacing the bulk DNA−surfactant solution with pure salt solution.American Chemical Society2001-03-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10625http://hdl.handle.net/10316/10625https://doi.org/10.1021/la000993eengLangmuir. 17:5 (2001) 1666-16690743-7463Eskilsson, K.Leal, C.Lindman, B.Miguel, M.Nylander, T.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-03-05T10:56:24Zoai:estudogeral.uc.pt:10316/10625Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:34.108249Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
DNA−Surfactant Complexes at Solid Surfaces |
title |
DNA−Surfactant Complexes at Solid Surfaces |
spellingShingle |
DNA−Surfactant Complexes at Solid Surfaces Eskilsson, K. |
title_short |
DNA−Surfactant Complexes at Solid Surfaces |
title_full |
DNA−Surfactant Complexes at Solid Surfaces |
title_fullStr |
DNA−Surfactant Complexes at Solid Surfaces |
title_full_unstemmed |
DNA−Surfactant Complexes at Solid Surfaces |
title_sort |
DNA−Surfactant Complexes at Solid Surfaces |
author |
Eskilsson, K. |
author_facet |
Eskilsson, K. Leal, C. Lindman, B. Miguel, M. Nylander, T. |
author_role |
author |
author2 |
Leal, C. Lindman, B. Miguel, M. Nylander, T. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Eskilsson, K. Leal, C. Lindman, B. Miguel, M. Nylander, T. |
description |
In this work, we report on the adsorption of complexes between DNA of different molecular weight and a cationic surfactant, cetyltrimethylammonium bromide (CTAB), on hydrophobized and hydrophilic negatively charged silica surfaces as measured by ellipsometry. We will demonstrate how the adsorption is affected by the state of the DNA−surfactant complexes formed in bulk solution. High molecular weight DNA molecules, which condense (transform from coil to globule state) on addition of small amounts of cationic surfactants, do not adsorb on hydrophilic silica prior to phase separation. However, DNA−surfactant complexes formed from low molecular weight DNA were found to adsorb. For these complexes surfactants interact with DNA, without condensation of the DNA. Adsorbed DNA−surfactant complexes can easily be removed from the hydrophilic silica surface when replacing the bulk DNA−surfactant solution with pure salt solution. At the hydrophobic surface the DNA adsorbs without addition of cationic surfactant. However, with addition of a very low amount of surfactant, a rapid increase in adsorbed amount and a simultaneous decrease in adsorbed layer thickness are observed. This compaction of the adsorbed layer is to some extent reversible when replacing the bulk DNA−surfactant solution with pure salt solution. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-03-06 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10625 http://hdl.handle.net/10316/10625 https://doi.org/10.1021/la000993e |
url |
http://hdl.handle.net/10316/10625 https://doi.org/10.1021/la000993e |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Langmuir. 17:5 (2001) 1666-1669 0743-7463 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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