Investigate the role of the transcription factor FBH1 mediating the Circadian Clock regulation of C4 photosynthesis

• Autor(a) principal: Gonçalves, Ivan Sandro Batista Guerreiro
• Data de Publicação: 2022
• Tipo de documento: Dissertação
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10362/163137
• topic: C4 photosynthesis; basic helix–loop–helix; circadian clock; PEPC

" Engineering C4 photosynthesis into rice, a C3 plant, is predicted to increase yield by 50%. We must understand how C4 photosynthesis is regulated, in particular how the C3 transcriptional machinery regulates C4 photosynthetic genes. In C4 plants, Phosphoenolpyruvate Carboxylase (PEPC) is crucial for the high assimilation of CO2 by Rubisco. Identification and characterization of C3 transcription factors (TF) that regulate C4PEPC expression, is an important step for installing a functional C4 photosynthetic pathway in rice. OsFBH1 has been shown to regulate two C4PEPC promoters, making it a very interesting TF to study. In arabidopsis, FBH1 regulates and is regulated by CCA1, a key component of the circadian oscillator. If this interaction is conserved in rice, OsFBH1 might be the link between circadian clock and C4 photosynthesis. In this study, we intended to investigate the reciprocal regulation of OsFBH1 and OsCCA1. Performing a time course experiment using OsCCA1 knockout lines, we observed an impairment in the expression of OsFBH1, demonstrating its regulation by OsCCA1.(...)"