The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis

Detalhes bibliográficos
Autor(a) principal: Costa, S
Data de Publicação: 2016
Outros Autores: Ceppi, M, Costa, C, Silva, S, Pereira, C, Laffon, B, Bonassi, S, Teixeira, JP
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10216/114782
Resumo: Styrene is a building-block of several compounds used in a wide array of materials and products. The most important human exposure to this substance occurs in industrial settings, especially among reinforced-plastics industry workers. The effect of occupational exposure to styrene on cytogenetics biomarkers has been previously reviewed with positive association observed for chromosomal aberrations, and inconclusive data for the micronucleus assay. Some limitations were noted in those studies, including inadequate exposure assessment and poor epidemiological design. Furthermore, in earlier studies micronuclei frequency was measured with protocols not as reliable as cytokinesis-block micronucleus (CBMN) assay. Aim of the present systematic review and meta-analysis is to investigate genomic instability and DNA damage as measured by the CBMN assay in lymphocytes of subjects exposed to styrene. A total of 11 studies published between 2004 and 2012 were included in the meta-analysis encompassing 479 styrene-exposed workers and 510 controls. The quality of each study was estimated by a quality scoring system which ranked studies according to the consideration of major confounders, exposure characterization, and technical parameters. An overall increase of micronuclei frequencies was found in styrene-exposure workers when compared to referents (meta-MR 1.34; 95% CI 1.18-1.52), with significant increases achieved in six individual studies. The consistency of results in individual studies, the independence of this result from major confounding factors and from the quality of the study strengthens the reliability of risk estimates and supports the use of the CBMN assay in monitoring genetic risk in styrene workers.
id RCAP_a0d6dcf1965ef4913c5b79ce27cd8c2f
oai_identifier_str oai:repositorio-aberto.up.pt:10216/114782
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysisCytokinesis-block micronucleusStyrene is a building-block of several compounds used in a wide array of materials and products. The most important human exposure to this substance occurs in industrial settings, especially among reinforced-plastics industry workers. The effect of occupational exposure to styrene on cytogenetics biomarkers has been previously reviewed with positive association observed for chromosomal aberrations, and inconclusive data for the micronucleus assay. Some limitations were noted in those studies, including inadequate exposure assessment and poor epidemiological design. Furthermore, in earlier studies micronuclei frequency was measured with protocols not as reliable as cytokinesis-block micronucleus (CBMN) assay. Aim of the present systematic review and meta-analysis is to investigate genomic instability and DNA damage as measured by the CBMN assay in lymphocytes of subjects exposed to styrene. A total of 11 studies published between 2004 and 2012 were included in the meta-analysis encompassing 479 styrene-exposed workers and 510 controls. The quality of each study was estimated by a quality scoring system which ranked studies according to the consideration of major confounders, exposure characterization, and technical parameters. An overall increase of micronuclei frequencies was found in styrene-exposure workers when compared to referents (meta-MR 1.34; 95% CI 1.18-1.52), with significant increases achieved in six individual studies. The consistency of results in individual studies, the independence of this result from major confounding factors and from the quality of the study strengthens the reliability of risk estimates and supports the use of the CBMN assay in monitoring genetic risk in styrene workers.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10216/114782eng1383-574210.1016/j.mrrev.2016.06.003Costa, SCeppi, MCosta, CSilva, SPereira, CLaffon, BBonassi, STeixeira, JPinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T15:09:03Zoai:repositorio-aberto.up.pt:10216/114782Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T00:16:50.538595Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
title The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
spellingShingle The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
Costa, S
Cytokinesis-block micronucleus
title_short The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
title_full The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
title_fullStr The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
title_full_unstemmed The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
title_sort The cytokinesis-block micronucleus (CBMN) assay in human populations exposed to styrene: A systematic review and meta-analysis
author Costa, S
author_facet Costa, S
Ceppi, M
Costa, C
Silva, S
Pereira, C
Laffon, B
Bonassi, S
Teixeira, JP
author_role author
author2 Ceppi, M
Costa, C
Silva, S
Pereira, C
Laffon, B
Bonassi, S
Teixeira, JP
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, S
Ceppi, M
Costa, C
Silva, S
Pereira, C
Laffon, B
Bonassi, S
Teixeira, JP
dc.subject.por.fl_str_mv Cytokinesis-block micronucleus
topic Cytokinesis-block micronucleus
description Styrene is a building-block of several compounds used in a wide array of materials and products. The most important human exposure to this substance occurs in industrial settings, especially among reinforced-plastics industry workers. The effect of occupational exposure to styrene on cytogenetics biomarkers has been previously reviewed with positive association observed for chromosomal aberrations, and inconclusive data for the micronucleus assay. Some limitations were noted in those studies, including inadequate exposure assessment and poor epidemiological design. Furthermore, in earlier studies micronuclei frequency was measured with protocols not as reliable as cytokinesis-block micronucleus (CBMN) assay. Aim of the present systematic review and meta-analysis is to investigate genomic instability and DNA damage as measured by the CBMN assay in lymphocytes of subjects exposed to styrene. A total of 11 studies published between 2004 and 2012 were included in the meta-analysis encompassing 479 styrene-exposed workers and 510 controls. The quality of each study was estimated by a quality scoring system which ranked studies according to the consideration of major confounders, exposure characterization, and technical parameters. An overall increase of micronuclei frequencies was found in styrene-exposure workers when compared to referents (meta-MR 1.34; 95% CI 1.18-1.52), with significant increases achieved in six individual studies. The consistency of results in individual studies, the independence of this result from major confounding factors and from the quality of the study strengthens the reliability of risk estimates and supports the use of the CBMN assay in monitoring genetic risk in styrene workers.
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10216/114782
url http://hdl.handle.net/10216/114782
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1383-5742
10.1016/j.mrrev.2016.06.003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799136088295473152