Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase

Detalhes bibliográficos
Autor(a) principal: Moura, Isabel
Data de Publicação: 2004
Outros Autores: Santos, Margarida M. Correia dos, Sousa, Patrícia M. P., Gonçalves, M. Lurdes S., Romão, M. João, Moura, José J. G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/1656
Resumo: Eur. J. Biochem. 271, 1329–1338 (2004)
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spelling Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductaseAldehyde oxidoreductaseDesulfovibrio gigasElectrochemistryEur. J. Biochem. 271, 1329–1338 (2004)This work reports on the direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase (DgAOR), a molybdenum enzyme of the xanthine oxidase family that contains three redox-active cofactors: two [2Fe-2S] centers and a molybdopterin cytosine dinucleotide cofactor. The voltammetric behavior of the enzyme was analyzed at gold and carbon (pyrolytic graphite and glassy carbon) electrodes. Two different strategies were used: one with the molecules confined to the electrode surface and a second with DgAOR in solution. In all of the cases studied, electron transfer took place, although different redox reactions were responsible for the voltammetric signal. From a thorough analysis of the voltammetric responses and the structural properties of the molecular surface of DgAOR, the redox reaction at the carbon electrodes could be assigned to the reduction of the more exposed iron cluster, [2Fe-2S] II, whereas reduction of the molybdopterin cofactor occurs at the gold electrode. Voltammetric results in the presence of aldehydes are also reported and discussed.Blackwell PublishingRUNMoura, IsabelSantos, Margarida M. Correia dosSousa, Patrícia M. P.Gonçalves, M. Lurdes S.Romão, M. JoãoMoura, José J. G.2008-09-23T13:21:33Z20042004-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/1656eng00142956info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:31:38Zoai:run.unl.pt:10362/1656Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:14:46.904248Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
title Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
spellingShingle Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
Moura, Isabel
Aldehyde oxidoreductase
Desulfovibrio gigas
Electrochemistry
title_short Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
title_full Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
title_fullStr Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
title_full_unstemmed Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
title_sort Direct electrochemistry of the Desulfovibrio gigas aldehyde oxidoreductase
author Moura, Isabel
author_facet Moura, Isabel
Santos, Margarida M. Correia dos
Sousa, Patrícia M. P.
Gonçalves, M. Lurdes S.
Romão, M. João
Moura, José J. G.
author_role author
author2 Santos, Margarida M. Correia dos
Sousa, Patrícia M. P.
Gonçalves, M. Lurdes S.
Romão, M. João
Moura, José J. G.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv RUN
dc.contributor.author.fl_str_mv Moura, Isabel
Santos, Margarida M. Correia dos
Sousa, Patrícia M. P.
Gonçalves, M. Lurdes S.
Romão, M. João
Moura, José J. G.
dc.subject.por.fl_str_mv Aldehyde oxidoreductase
Desulfovibrio gigas
Electrochemistry
topic Aldehyde oxidoreductase
Desulfovibrio gigas
Electrochemistry
description Eur. J. Biochem. 271, 1329–1338 (2004)
publishDate 2004
dc.date.none.fl_str_mv 2004
2004-01-01T00:00:00Z
2008-09-23T13:21:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/1656
url http://hdl.handle.net/10362/1656
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 00142956
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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