Fab antibody fragment-functionalized liposomes for specific targeting of antigen-positive cells

• Autor(a) principal: Ohradanova-Repic, Anna
• Data de Publicação: 2018
• Outros Autores: Nogueira, E., Hartl, Ingrid, Gomes, Andreia C., Preto, Ana, Steinhuber, Eva, Mühlgrabner, Vanessa, Repic, Marko, Kuttke, Mario, Zwirzitz, Alexander, Prouza, Marek, Suchanek, Miloslav, Wozniak-Knopp, Gordana, Horejsi, Vaclav, Schabbauer, Gernot, Cavaco-Paulo, Artur, Stockinger, Hannes
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/1822/51164
• topic: active targeting; liposome functionalization; immunoliposome; antibody engineering; recombinant Fab antibody fragment; Science & Technology

Liposomes functionalized with monoclonal antibodies or their antigen-binding fragments have attracted much attention as specific drug delivery devices for treatment of various diseases including cancer. The conjugation of antibodies to liposomes is usually achieved by covalent coupling using cross-linkers in a reaction that might adversely affect the characteristics of the final product. Here we present an alternative strategy for liposome functionalization: we created a recombinant Fab antibody fragment genetically fused on its C-terminus to the hydrophobic peptide derived from pulmonary surfactant protein D, which became inserted into the liposomal bilayer during liposomal preparation and anchored the Fab onto the liposome surface. The Fab-conjugated liposomes specifically recognized antigen-positive cells and efficiently delivered their cargo, the Alexa Fluor 647 dye, into target cells in vitro and in vivo. In conclusion, our approach offers the potential for straightforward development of nanomedicines functionalized with an antibody of choice without the need of harmful cross-linkers.