The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation

Detalhes bibliográficos
Autor(a) principal: Díaz, E.
Data de Publicação: 2017
Outros Autores: Puerto, I., Ribeiro, Sylvie Oliveira, Lanceros-Méndez, S., Barandiarán, J. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/48493
Resumo: The influence of copolymer composition on Poly(Lactide-co-Glycolide)/ nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 °C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications.
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spelling The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradationScaffoldsPLGAnHACytotoxicityIn vitro degradationCiências Naturais::Ciências FísicasScience & TechnologyThe influence of copolymer composition on Poly(Lactide-co-Glycolide)/ nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 °C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications.SGIker (UPV/EHU, MICINN, GV/EJ, ERDF and ESF) is really appreciated. This work was supported by the Portuguese Foundation for Science and Technology (FCT) in the framework of the Strategic Funding UID/FIS/04650/2013 and Grant SFRH/BD/111478/2015 (S.R). The authors acknowledge funding by the Spanish Ministry of Economy and Competitiveness (MINECO) through the project MAT2016-76039-C4-3-R (Agencia Estatal de Investigación(AEI)/FEDER, Unión Europea(UE))info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoDíaz, E.Puerto, I.Ribeiro, Sylvie OliveiraLanceros-Méndez, S.Barandiarán, J. M.2017-072017-07-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/48493engDíaz, E., Puerto, I., Ribeiro, S., Lanceros-Mendez, S., & Barandiarán, J. M. (2017). The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation. Nanomaterials, 7(7), 1732079-49912079-499110.3390/nano7070173http://www.mdpi.com/2079-4991/7/7/173/htminfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:56:30Zoai:repositorium.sdum.uminho.pt:1822/48493Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:46:06.851570Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
title The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
spellingShingle The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
Díaz, E.
Scaffolds
PLGA
nHA
Cytotoxicity
In vitro degradation
Ciências Naturais::Ciências Físicas
Science & Technology
title_short The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
title_full The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
title_fullStr The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
title_full_unstemmed The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
title_sort The influence of copolymer composition on PLGA/nHA scaffolds´ cytotoxicity and in vitro degradation
author Díaz, E.
author_facet Díaz, E.
Puerto, I.
Ribeiro, Sylvie Oliveira
Lanceros-Méndez, S.
Barandiarán, J. M.
author_role author
author2 Puerto, I.
Ribeiro, Sylvie Oliveira
Lanceros-Méndez, S.
Barandiarán, J. M.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Díaz, E.
Puerto, I.
Ribeiro, Sylvie Oliveira
Lanceros-Méndez, S.
Barandiarán, J. M.
dc.subject.por.fl_str_mv Scaffolds
PLGA
nHA
Cytotoxicity
In vitro degradation
Ciências Naturais::Ciências Físicas
Science & Technology
topic Scaffolds
PLGA
nHA
Cytotoxicity
In vitro degradation
Ciências Naturais::Ciências Físicas
Science & Technology
description The influence of copolymer composition on Poly(Lactide-co-Glycolide)/ nanohydroxyapatite (PLGA/nHA) composite scaffolds is studied in the context of bone tissue engineering and regenerative medicine. The composite scaffolds are fabricated by thermally-induced phase separation and the effect of bioactive nanoparticles on their in vitro degradation in phosphate-buffered solution at 37 °C is analyzed over eight weeks. The indirect cytotoxicity evaluation of the samples followed an adaptation of the ISO 10993-5 standard test method. Based on the measurement of their molecular weight, molar mass, pH, water absorption and dimensions, the porous scaffolds of PLGA with a lower lactide/glycolide (LA/GA) molar ratio degraded faster due to their higher hydrophilicity. All of the samples without and with HA are not cytotoxic, demonstrating their potential for tissue engineering applications.
publishDate 2017
dc.date.none.fl_str_mv 2017-07
2017-07-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/48493
url http://hdl.handle.net/1822/48493
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Díaz, E., Puerto, I., Ribeiro, S., Lanceros-Mendez, S., & Barandiarán, J. M. (2017). The Influence of Copolymer Composition on PLGA/nHA Scaffolds’ Cytotoxicity and In Vitro Degradation. Nanomaterials, 7(7), 173
2079-4991
2079-4991
10.3390/nano7070173
http://www.mdpi.com/2079-4991/7/7/173/htm
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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