Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)

Detalhes bibliográficos
Autor(a) principal: Brandão, Fátima
Data de Publicação: 2020
Outros Autores: Fernández-Bertólez, Natalia, Rosário, Fernanda, Bessa, Maria João, Fraga, Sónia, Pásaro, Eduardo, Teixeira, João Paulo, Laffon, Blanca, Valdiglesias, Vanessa, Costa, Carla
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/7302
Resumo: This article belongs to the Special Issue Toxicity and Ecotoxicity Assessment of Nanomaterials by In Vitro Models
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spelling Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)A172A549HepG2SH-SY5YMicronucleiTitanium Dioxide NanoparticlesTiO2 NPsUptakeGenotoxicidade AmbientalThis article belongs to the Special Issue Toxicity and Ecotoxicity Assessment of Nanomaterials by In Vitro ModelsTitanium dioxide nanoparticles (TiO2 NPs) have a wide variety of applications in many consumer products, including as food additives, increasing the concern about the possible hazards that TiO2 NPs may pose to human health. Although most previous studies have focused on the respiratory system, ingestion must also be considered as an important exposure route. Furthermore, after inhalation or ingestion, TiO2 NPs can reach several organs, such as the liver, brain or lungs. Taking this into consideration, the present study focuses on the uptake and potential genotoxicity (micronuclei induction) of TiO2 NPs on four human cell lines of diverse origin: lung cells (A549), liver cells (HepG2), glial cells (A172) and neurons (SH-SY5Y), using flow cytometry methods. Results showed a concentration-, time- and cell-type- dependent increase in TiO2 NPs uptake but no significant induction of micronuclei in any of the tested conditions. Data obtained reinforce the importance of cell model and testing protocols choice for toxicity assessment. However, some questions remain to be answered, namely on the role of cell culture media components on the agglomeration state and mitigation of TiO2 NPs toxic effects.The present work was supported by Xunta de Galicia (reference ED431B 2019/02), NanoBioBarriers (PTDC/MED-TOX/31162/2017) funded by Operational Program for Competitiveness and Internationalisation through European Regional Development Funds (FEDER/FNR) and through national funds by the Portuguese Foundation for Science and Technology (FCT) and NanoLegaTox (PTDC/SAU-PUB/29651/2017) project co-financed by COMPETE 2020, Portugal 2020 and European Union, through FEDER. F.B. are M.J.B. recipients of FCT fellowships (SFRH/BD/101060/2014 and SFRH/BD/12046/2016); V.V. is supported by a Beatriz Galindo Research Fellowship (reference BEAGAL18/00142); and NF-B by a Deputación Provincial da Coruña fellowship (2019000011550).MDPIRepositório Científico do Instituto Nacional de SaúdeBrandão, FátimaFernández-Bertólez, NataliaRosário, FernandaBessa, Maria JoãoFraga, SóniaPásaro, EduardoTeixeira, João PauloLaffon, BlancaValdiglesias, VanessaCosta, Carla2021-03-04T10:57:53Z2020-02-272020-02-27T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/7302engNanomaterials (Basel). 2020 Feb 27;10(3):412. doi: 10.3390/nano100304122079-499110.3390/nano10030412info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:42:01Zoai:repositorio.insa.pt:10400.18/7302Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:42:06.281237Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
title Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
spellingShingle Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
Brandão, Fátima
A172
A549
HepG2
SH-SY5Y
Micronuclei
Titanium Dioxide Nanoparticles
TiO2 NPs
Uptake
Genotoxicidade Ambiental
title_short Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
title_full Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
title_fullStr Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
title_full_unstemmed Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
title_sort Genotoxicity of TiO2 Nanoparticles in Four Different Human Cell Lines (A549, HEPG2, A172 and SH-SY5Y)
author Brandão, Fátima
author_facet Brandão, Fátima
Fernández-Bertólez, Natalia
Rosário, Fernanda
Bessa, Maria João
Fraga, Sónia
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
Costa, Carla
author_role author
author2 Fernández-Bertólez, Natalia
Rosário, Fernanda
Bessa, Maria João
Fraga, Sónia
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
Costa, Carla
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Brandão, Fátima
Fernández-Bertólez, Natalia
Rosário, Fernanda
Bessa, Maria João
Fraga, Sónia
Pásaro, Eduardo
Teixeira, João Paulo
Laffon, Blanca
Valdiglesias, Vanessa
Costa, Carla
dc.subject.por.fl_str_mv A172
A549
HepG2
SH-SY5Y
Micronuclei
Titanium Dioxide Nanoparticles
TiO2 NPs
Uptake
Genotoxicidade Ambiental
topic A172
A549
HepG2
SH-SY5Y
Micronuclei
Titanium Dioxide Nanoparticles
TiO2 NPs
Uptake
Genotoxicidade Ambiental
description This article belongs to the Special Issue Toxicity and Ecotoxicity Assessment of Nanomaterials by In Vitro Models
publishDate 2020
dc.date.none.fl_str_mv 2020-02-27
2020-02-27T00:00:00Z
2021-03-04T10:57:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/7302
url http://hdl.handle.net/10400.18/7302
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nanomaterials (Basel). 2020 Feb 27;10(3):412. doi: 10.3390/nano10030412
2079-4991
10.3390/nano10030412
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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