Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.

Detalhes bibliográficos
Autor(a) principal: Siqueira, Maria Cíntia
Data de Publicação: 2002
Outros Autores: Morgado, Fernando
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1913
Resumo: L-carnitine plays a major role in the transport of the long chain fatty acids for the beta-oxidation in the mitochondria. The deficiency of the carnitine is associated by the deficit of the beta-oxidation of the long chain fatty acids and so a different syndromes. The purpose of this study was to determine the plasmatic concentrations of the carnitine and its metabolites in patients with neuromuscular pathologies within the group of mitochondrial myopathies by the comparison with a control group. The study comprised 11 healthy volunteers and 11 patients with neuromuscular mitochondrial diseases. The plasmatic concentrations of the free carnitine, total carnitine and the short and long chain acilcarnitine were determined by using the radio enzymatic method with Acetyl Co A 14C. The values were calculated by the standard curve in increased concentrations of the L-carnitine in aquous solution. RESULTS: The values were expressed as mean +/- S.D. The control group gave the following values: For free carnitine (32.6 +/- 4.95 microM), total carnitine (38.48 +/- 5.8 microM), short chain acylcarnitine (4.12 +/- 0.95 microM) and for long chain acylcarnitine (1.73 +/- 0.15 microM). The patients were divided in two groups according to their values: The 1st group gave the values within the control group: For free carnitine (31.44 +/- 3.72 microM), total carnitine (36.6 +/- 3.86 microM), short chain acylcarnitine (3.52 +/- 1.56 microM) and long chain acylcarnitine (1.68 +/- 0.08 microM). The 2nd group gave values bellow the control group: For free carnitine (16.8 +/- 6.3 microM), total carnitine (20.88 +/- 6.26 microM), short chain acylcarnitine (2.98 +/- 0.81 microM) and long chain acylcarnitine (0.92 +/- 0.41 microM) respectively. In conclusion, this method showed appropriate and accurate for the determinations of the carnitine and its metabolites in plasma, and must be useful for clinical support.
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spelling Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.Determinação dos níveis da carnitina no plasma sua importância no estudo em doentes com doenças neuromusculares.L-carnitine plays a major role in the transport of the long chain fatty acids for the beta-oxidation in the mitochondria. The deficiency of the carnitine is associated by the deficit of the beta-oxidation of the long chain fatty acids and so a different syndromes. The purpose of this study was to determine the plasmatic concentrations of the carnitine and its metabolites in patients with neuromuscular pathologies within the group of mitochondrial myopathies by the comparison with a control group. The study comprised 11 healthy volunteers and 11 patients with neuromuscular mitochondrial diseases. The plasmatic concentrations of the free carnitine, total carnitine and the short and long chain acilcarnitine were determined by using the radio enzymatic method with Acetyl Co A 14C. The values were calculated by the standard curve in increased concentrations of the L-carnitine in aquous solution. RESULTS: The values were expressed as mean +/- S.D. The control group gave the following values: For free carnitine (32.6 +/- 4.95 microM), total carnitine (38.48 +/- 5.8 microM), short chain acylcarnitine (4.12 +/- 0.95 microM) and for long chain acylcarnitine (1.73 +/- 0.15 microM). The patients were divided in two groups according to their values: The 1st group gave the values within the control group: For free carnitine (31.44 +/- 3.72 microM), total carnitine (36.6 +/- 3.86 microM), short chain acylcarnitine (3.52 +/- 1.56 microM) and long chain acylcarnitine (1.68 +/- 0.08 microM). The 2nd group gave values bellow the control group: For free carnitine (16.8 +/- 6.3 microM), total carnitine (20.88 +/- 6.26 microM), short chain acylcarnitine (2.98 +/- 0.81 microM) and long chain acylcarnitine (0.92 +/- 0.41 microM) respectively. In conclusion, this method showed appropriate and accurate for the determinations of the carnitine and its metabolites in plasma, and must be useful for clinical support.L-carnitine plays a major role in the transport of the long chain fatty acids for the beta-oxidation in the mitochondria. The deficiency of the carnitine is associated by the deficit of the beta-oxidation of the long chain fatty acids and so a different syndromes. The purpose of this study was to determine the plasmatic concentrations of the carnitine and its metabolites in patients with neuromuscular pathologies within the group of mitochondrial myopathies by the comparison with a control group. The study comprised 11 healthy volunteers and 11 patients with neuromuscular mitochondrial diseases. The plasmatic concentrations of the free carnitine, total carnitine and the short and long chain acilcarnitine were determined by using the radio enzymatic method with Acetyl Co A 14C. The values were calculated by the standard curve in increased concentrations of the L-carnitine in aquous solution. RESULTS: The values were expressed as mean +/- S.D. The control group gave the following values: For free carnitine (32.6 +/- 4.95 microM), total carnitine (38.48 +/- 5.8 microM), short chain acylcarnitine (4.12 +/- 0.95 microM) and for long chain acylcarnitine (1.73 +/- 0.15 microM). The patients were divided in two groups according to their values: The 1st group gave the values within the control group: For free carnitine (31.44 +/- 3.72 microM), total carnitine (36.6 +/- 3.86 microM), short chain acylcarnitine (3.52 +/- 1.56 microM) and long chain acylcarnitine (1.68 +/- 0.08 microM). The 2nd group gave values bellow the control group: For free carnitine (16.8 +/- 6.3 microM), total carnitine (20.88 +/- 6.26 microM), short chain acylcarnitine (2.98 +/- 0.81 microM) and long chain acylcarnitine (0.92 +/- 0.41 microM) respectively. In conclusion, this method showed appropriate and accurate for the determinations of the carnitine and its metabolites in plasma, and must be useful for clinical support.Ordem dos Médicos2002-02-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1913oai:ojs.www.actamedicaportuguesa.com:article/1913Acta Médica Portuguesa; Vol. 15 No. 1 (2002): Janeiro-Fevereiro; 11-15Acta Médica Portuguesa; Vol. 15 N.º 1 (2002): Janeiro-Fevereiro; 11-151646-07580870-399Xreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPporhttps://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1913https://www.actamedicaportuguesa.com/revista/index.php/amp/article/view/1913/1481Siqueira, Maria CíntiaMorgado, Fernandoinfo:eu-repo/semantics/openAccess2022-12-20T10:59:30Zoai:ojs.www.actamedicaportuguesa.com:article/1913Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:17:28.673086Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
Determinação dos níveis da carnitina no plasma sua importância no estudo em doentes com doenças neuromusculares.
title Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
spellingShingle Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
Siqueira, Maria Cíntia
title_short Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
title_full Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
title_fullStr Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
title_full_unstemmed Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
title_sort Determination of carnitine levels in plasma: importance in the study of patients with neuromuscular diseases.
author Siqueira, Maria Cíntia
author_facet Siqueira, Maria Cíntia
Morgado, Fernando
author_role author
author2 Morgado, Fernando
author2_role author
dc.contributor.author.fl_str_mv Siqueira, Maria Cíntia
Morgado, Fernando
description L-carnitine plays a major role in the transport of the long chain fatty acids for the beta-oxidation in the mitochondria. The deficiency of the carnitine is associated by the deficit of the beta-oxidation of the long chain fatty acids and so a different syndromes. The purpose of this study was to determine the plasmatic concentrations of the carnitine and its metabolites in patients with neuromuscular pathologies within the group of mitochondrial myopathies by the comparison with a control group. The study comprised 11 healthy volunteers and 11 patients with neuromuscular mitochondrial diseases. The plasmatic concentrations of the free carnitine, total carnitine and the short and long chain acilcarnitine were determined by using the radio enzymatic method with Acetyl Co A 14C. The values were calculated by the standard curve in increased concentrations of the L-carnitine in aquous solution. RESULTS: The values were expressed as mean +/- S.D. The control group gave the following values: For free carnitine (32.6 +/- 4.95 microM), total carnitine (38.48 +/- 5.8 microM), short chain acylcarnitine (4.12 +/- 0.95 microM) and for long chain acylcarnitine (1.73 +/- 0.15 microM). The patients were divided in two groups according to their values: The 1st group gave the values within the control group: For free carnitine (31.44 +/- 3.72 microM), total carnitine (36.6 +/- 3.86 microM), short chain acylcarnitine (3.52 +/- 1.56 microM) and long chain acylcarnitine (1.68 +/- 0.08 microM). The 2nd group gave values bellow the control group: For free carnitine (16.8 +/- 6.3 microM), total carnitine (20.88 +/- 6.26 microM), short chain acylcarnitine (2.98 +/- 0.81 microM) and long chain acylcarnitine (0.92 +/- 0.41 microM) respectively. In conclusion, this method showed appropriate and accurate for the determinations of the carnitine and its metabolites in plasma, and must be useful for clinical support.
publishDate 2002
dc.date.none.fl_str_mv 2002-02-27
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dc.publisher.none.fl_str_mv Ordem dos Médicos
publisher.none.fl_str_mv Ordem dos Médicos
dc.source.none.fl_str_mv Acta Médica Portuguesa; Vol. 15 No. 1 (2002): Janeiro-Fevereiro; 11-15
Acta Médica Portuguesa; Vol. 15 N.º 1 (2002): Janeiro-Fevereiro; 11-15
1646-0758
0870-399X
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