Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Tipo de documento: | Dissertação |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/158612 |
Resumo: | Estuaries are heavily impacted by anthropogenic contamination, serving as reservoirs of various pollutants such as heavy metals (e.g., mercury (Hg) and microplastics (MP, Ø <5mm). These pollutants can affect water quality and cause toxic effects in aquatic organisms. Mercury, a trace heavy metal, is characterized as neurotoxic, persistent, and bioaccumulative in the food chain. In systems contaminated by Hg, microorganisms are responsible for reducing Hg, contributing to its natural detoxification. However, the effects of the presence of MP in this process are still unknown. The growing concern of microplastics is the chemical additives in their constitution that can be released and their ability to act as vectors of other contaminants, namely Hg. Therefore, the following questions arise 1. The presence of microplastics affects bacterial and fungal-mediated mercury detoxification present in aquatic systems, and how this differs between the two microorganisms, and 2. What are the possible bioremediation strategies. Microbial communities (bacteria and fungi) were isolated from a Hg-contaminated area of the Tagus estuary, and the most resistant strains were selected to assess the effect of the presence of polystyrene (PS) MP (40 mg/L) on the detoxification of Hg. After 24h, 3, and 5 days of incubation for bacterial isolates and 24h, 5 days, and 10 days of incubation for fungal isolates, the following factors were analyzed: Hg detoxification capacity, acute toxicity (Microtox bioassay) of the leachate, and variation of pH. Isolates were identified through the amplification of 16SrRNA and ITS, respectively. The results showed that (i) strains isolated from the Tagus Estuary are resistant to Hg, (ii) fungal isolates are more resistant than bacteria and, consequently, have a greater capacity to detoxify Hg, (ii) the presence of PS MPs affect the availability of Hg and bacteria in the medium. It can be concluded that MPs interact with Hg detoxification processes and, therefore, should be considered in bioremediation strategies. |
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Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and FungiMercuryMicroplasticMicroorganismsDetoxificationBioremediationDomínio/Área Científica::Engenharia e Tecnologia::Engenharia do AmbienteEstuaries are heavily impacted by anthropogenic contamination, serving as reservoirs of various pollutants such as heavy metals (e.g., mercury (Hg) and microplastics (MP, Ø <5mm). These pollutants can affect water quality and cause toxic effects in aquatic organisms. Mercury, a trace heavy metal, is characterized as neurotoxic, persistent, and bioaccumulative in the food chain. In systems contaminated by Hg, microorganisms are responsible for reducing Hg, contributing to its natural detoxification. However, the effects of the presence of MP in this process are still unknown. The growing concern of microplastics is the chemical additives in their constitution that can be released and their ability to act as vectors of other contaminants, namely Hg. Therefore, the following questions arise 1. The presence of microplastics affects bacterial and fungal-mediated mercury detoxification present in aquatic systems, and how this differs between the two microorganisms, and 2. What are the possible bioremediation strategies. Microbial communities (bacteria and fungi) were isolated from a Hg-contaminated area of the Tagus estuary, and the most resistant strains were selected to assess the effect of the presence of polystyrene (PS) MP (40 mg/L) on the detoxification of Hg. After 24h, 3, and 5 days of incubation for bacterial isolates and 24h, 5 days, and 10 days of incubation for fungal isolates, the following factors were analyzed: Hg detoxification capacity, acute toxicity (Microtox bioassay) of the leachate, and variation of pH. Isolates were identified through the amplification of 16SrRNA and ITS, respectively. The results showed that (i) strains isolated from the Tagus Estuary are resistant to Hg, (ii) fungal isolates are more resistant than bacteria and, consequently, have a greater capacity to detoxify Hg, (ii) the presence of PS MPs affect the availability of Hg and bacteria in the medium. It can be concluded that MPs interact with Hg detoxification processes and, therefore, should be considered in bioremediation strategies.Os estuários são fortemente impactados pela contaminação antropogénica, servindo de reservatórios de diversos poluentes tais como metais pesados (ex: mercúrio (Hg) e microplásticos (MP, Ø <5mm). Estes poluentes podem afetar a qualidade da água e causar efeitos tóxicos nos organismos aquáticos. O mercúrio, um metal pesado vestigial, é caracterizado como neurotóxico, persistente e bioacumulativo na cadeia alimentar. Nos sistemas contaminados pelo Hg, os microrganismos são responsáveis pela redução de Hg, contribuindo para a destoxificação natural. No entanto, não se conhecem os efeitos da presença MP neste processo. A crescente preocupação dos microplásticos diz respeito não apenas aos aditivos químicos em sua constituição que podem ser libertados, mas também à sua capacidade de atuar como vetores de outros contaminantes, nomeadamente o Hg. Portanto, surgem as seguintes questões: 1. A presença de microplásticos afeta a desintoxicação do mercúrio mediada por bactérias e fungos presentes nos sistemas aquáticos, e como isso difere entre os dois microrganismos, e 2. Quais são as possíveis estratégias de biorremediação. Comunidades microbianas (bactérias e fungos) foram isoladas de uma área contaminada por Hg do estuário do Tejo, e as estirpes mais resistentes foram selecionadas para avaliar o efeito da presença de poliestireno (PS) MP (40 mg/L) na destoxificação de Hg. Após 24h, 3 e 5 dias de incubação para isolados de bactérias e 24h, 5 dias e 10 dias de incubação para isolados de fungos. foram analisados os seguintes fatores: capacidade de destoxificação de Hg, toxicidade aguda (bioensaio Microtox) do lixiviado e variação de pH. Os isolados foram identificados através da amplificação do 16SrRNA e ITS., respetivamente. Os resultados demonstraram que (i) estirpes isoladas do Estuário do Tejo apresentam resistência ao Hg, (ii) isolados de fungos são mais resistentes do que bactérias e, consequentemente, apresentam maior capacidade de destoxificação de Hg, (ii) a presença de PS MPs afeta a disponibilidade de Hg e de bactérias no meio. Pode-se concluir que os MPs interagem nos processos de destoxificação de Hg e, portanto, devem ser considerados nas estratégias de biorremediação.Martins, MartaFigueiredo, NeusaRUNSilva, Laura Tiê Zanchi da2023-10-03T14:36:26Z2022-012022-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/158612enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:09Zoai:run.unl.pt:10362/158612Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:14.256080Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
title |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
spellingShingle |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi Silva, Laura Tiê Zanchi da Mercury Microplastic Microorganisms Detoxification Bioremediation Domínio/Área Científica::Engenharia e Tecnologia::Engenharia do Ambiente |
title_short |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
title_full |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
title_fullStr |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
title_full_unstemmed |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
title_sort |
Evaluation of the Impact of Microplastics on Mercury Detoxification Strategies: a Comparison between the Performance of Bacteria and Fungi |
author |
Silva, Laura Tiê Zanchi da |
author_facet |
Silva, Laura Tiê Zanchi da |
author_role |
author |
dc.contributor.none.fl_str_mv |
Martins, Marta Figueiredo, Neusa RUN |
dc.contributor.author.fl_str_mv |
Silva, Laura Tiê Zanchi da |
dc.subject.por.fl_str_mv |
Mercury Microplastic Microorganisms Detoxification Bioremediation Domínio/Área Científica::Engenharia e Tecnologia::Engenharia do Ambiente |
topic |
Mercury Microplastic Microorganisms Detoxification Bioremediation Domínio/Área Científica::Engenharia e Tecnologia::Engenharia do Ambiente |
description |
Estuaries are heavily impacted by anthropogenic contamination, serving as reservoirs of various pollutants such as heavy metals (e.g., mercury (Hg) and microplastics (MP, Ø <5mm). These pollutants can affect water quality and cause toxic effects in aquatic organisms. Mercury, a trace heavy metal, is characterized as neurotoxic, persistent, and bioaccumulative in the food chain. In systems contaminated by Hg, microorganisms are responsible for reducing Hg, contributing to its natural detoxification. However, the effects of the presence of MP in this process are still unknown. The growing concern of microplastics is the chemical additives in their constitution that can be released and their ability to act as vectors of other contaminants, namely Hg. Therefore, the following questions arise 1. The presence of microplastics affects bacterial and fungal-mediated mercury detoxification present in aquatic systems, and how this differs between the two microorganisms, and 2. What are the possible bioremediation strategies. Microbial communities (bacteria and fungi) were isolated from a Hg-contaminated area of the Tagus estuary, and the most resistant strains were selected to assess the effect of the presence of polystyrene (PS) MP (40 mg/L) on the detoxification of Hg. After 24h, 3, and 5 days of incubation for bacterial isolates and 24h, 5 days, and 10 days of incubation for fungal isolates, the following factors were analyzed: Hg detoxification capacity, acute toxicity (Microtox bioassay) of the leachate, and variation of pH. Isolates were identified through the amplification of 16SrRNA and ITS, respectively. The results showed that (i) strains isolated from the Tagus Estuary are resistant to Hg, (ii) fungal isolates are more resistant than bacteria and, consequently, have a greater capacity to detoxify Hg, (ii) the presence of PS MPs affect the availability of Hg and bacteria in the medium. It can be concluded that MPs interact with Hg detoxification processes and, therefore, should be considered in bioremediation strategies. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01 2022-01-01T00:00:00Z 2023-10-03T14:36:26Z |
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info:eu-repo/semantics/publishedVersion |
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http://hdl.handle.net/10362/158612 |
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eng |
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eng |
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