Controlled adsorption of cytochrome c to nanostructured gold surfaces

Detalhes bibliográficos
Autor(a) principal: Gomes, Inês
Data de Publicação: 2012
Outros Autores: Feio, Maria J., Santos, Nuno C., Eaton, Peter, Serro, Ana Paula, Saramago, Benilde, Pereira, Eulália, Franco, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/10762
Resumo: © Springer Science+Business Media Dordrecht 2012
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spelling Controlled adsorption of cytochrome c to nanostructured gold surfacesSelf-assembled monolayersAlkanethiolsGold surfacesGold nanoparticlesCytochrome cQuartz crystal microbalance© Springer Science+Business Media Dordrecht 2012Controlled electrostatic physisorption of horse heart cytochrome c (Cyt c) onto nanostructured gold surfaces was investigated using Quartz-Crystal Microbalance measurements in planar gold surfaces with or without functionalization using a self-assembled monolayer (SAM) of the alkanethiol mercaptoundecanoic acid (MUA). MUA is a useful functionalization ligand for gold surfaces, shedding adsorbed biomolecules from the excessive electron density of the metal. A parallel analysis was conducted in the corresponding curved surfaces of 15nm gold nanoparticles (AuNPs), using zeta-potential and UV– visible spectroscopy. Atomic Force Microscopy of both types of functionalized gold surfaces with a MUA SAM, allowed for visualization of Cyt c deposits on the nanostructured gold surface. The amount of Cyt c adsorbed onto the gold surface could be controlled by the solution pH. For the assays conducted at pH 4.5, when MUA SAM-functionalized planar gold surfaces are positive or neutral, and Cyt c has a positive net charge, only 13% of the planar gold surface area was coated with protein. In contrast, at pH 7.4, when MUA SAM-functionalized planar gold surfaces and Cyt c have opposite charges, a protein coverage of 28% could be observed implying an adsorption process strongly governed by electrostatic forces. Cyt c adsorption on planar and curved gold surfaces are found to be greatly favored by the presence of a MUA-capping layer. In particular, on the AuNPs, the binding constant is three times larger than the binding constant obtained for the original citrate-capped AuNPs.Financial support was provided by Fundação para a Ciência e a Tecnologia (FCT/MEC), Portugal, under Grants PEst-C/EQB/LA0006/2011 to MJF, PE, EP, and RF; and PTDC/CTM-NAN/112241/2009 to RF; and PEst-OE/QUI/UI0100/2011 to APS and BS. IG is a recipient of a FCT/MEC post-doctoral fellowship (SFRH/BPD/63850/2009).SpringerRepositório da Universidade de LisboaGomes, InêsFeio, Maria J.Santos, Nuno C.Eaton, PeterSerro, Ana PaulaSaramago, BenildePereira, EuláliaFranco, Ricardo2014-03-21T10:58:14Z20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/10762engJ Nanopart Res (2012) 14:13211388-0764http://dx.doi.org/10.1007/s11051-012-1321-7metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-11-20T17:14:44Zoai:repositorio.ul.pt:10451/10762Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-11-20T17:14:44Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Controlled adsorption of cytochrome c to nanostructured gold surfaces
title Controlled adsorption of cytochrome c to nanostructured gold surfaces
spellingShingle Controlled adsorption of cytochrome c to nanostructured gold surfaces
Gomes, Inês
Self-assembled monolayers
Alkanethiols
Gold surfaces
Gold nanoparticles
Cytochrome c
Quartz crystal microbalance
title_short Controlled adsorption of cytochrome c to nanostructured gold surfaces
title_full Controlled adsorption of cytochrome c to nanostructured gold surfaces
title_fullStr Controlled adsorption of cytochrome c to nanostructured gold surfaces
title_full_unstemmed Controlled adsorption of cytochrome c to nanostructured gold surfaces
title_sort Controlled adsorption of cytochrome c to nanostructured gold surfaces
author Gomes, Inês
author_facet Gomes, Inês
Feio, Maria J.
Santos, Nuno C.
Eaton, Peter
Serro, Ana Paula
Saramago, Benilde
Pereira, Eulália
Franco, Ricardo
author_role author
author2 Feio, Maria J.
Santos, Nuno C.
Eaton, Peter
Serro, Ana Paula
Saramago, Benilde
Pereira, Eulália
Franco, Ricardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Gomes, Inês
Feio, Maria J.
Santos, Nuno C.
Eaton, Peter
Serro, Ana Paula
Saramago, Benilde
Pereira, Eulália
Franco, Ricardo
dc.subject.por.fl_str_mv Self-assembled monolayers
Alkanethiols
Gold surfaces
Gold nanoparticles
Cytochrome c
Quartz crystal microbalance
topic Self-assembled monolayers
Alkanethiols
Gold surfaces
Gold nanoparticles
Cytochrome c
Quartz crystal microbalance
description © Springer Science+Business Media Dordrecht 2012
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
2014-03-21T10:58:14Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/10762
url http://hdl.handle.net/10451/10762
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Nanopart Res (2012) 14:1321
1388-0764
http://dx.doi.org/10.1007/s11051-012-1321-7
dc.rights.driver.fl_str_mv metadata only access
info:eu-repo/semantics/openAccess
rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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