Current trends on protein driven bioinks for 3D printing

Detalhes bibliográficos
Autor(a) principal: Veiga, Anabela
Data de Publicação: 2021
Outros Autores: Silva, Inês V., Duarte, Marta M., Oliveira, Ana L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.14/35299
Resumo: In the last decade, three-dimensional (3D) extrusion bioprinting has been on the top trend for innovative technologies in the field of biomedical engineering. In particular, protein-based bioinks such as collagen, gelatin, silk fibroin, elastic, fibrin and protein complexes based on decellularized extracellular matrix (dECM) are receiving increasing attention. This current interest is the result of protein’s tunable properties, biocompatibility, environmentally friendly nature and possibility to provide cells with the adequate cues, mimicking the extracellular matrix’s function. In this review we describe the most relevant stages of the development of a protein-driven bioink. The most popular formulations, molecular weights and extraction methods are covered. The different crosslinking methods used in protein bioinks, the formulation with other polymeric systems or molecules of interest as well as the bioprinting settings are herein highlighted. The cell embedding procedures, the in vitro, in vivo, in situ studies and final applications are also discussed. Finally, we approach the development and optimization of bioinks from a sequential perspective, discussing the relevance of each parameter during the pre-processing, processing, and post-processing stages of technological development. Through this approach the present review expects to provide, in a sequential manner, helpful methodological guidelines for the development of novel bioinks.
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spelling Current trends on protein driven bioinks for 3D printingBioinkExtrusion bioprintingProtein-basedIn the last decade, three-dimensional (3D) extrusion bioprinting has been on the top trend for innovative technologies in the field of biomedical engineering. In particular, protein-based bioinks such as collagen, gelatin, silk fibroin, elastic, fibrin and protein complexes based on decellularized extracellular matrix (dECM) are receiving increasing attention. This current interest is the result of protein’s tunable properties, biocompatibility, environmentally friendly nature and possibility to provide cells with the adequate cues, mimicking the extracellular matrix’s function. In this review we describe the most relevant stages of the development of a protein-driven bioink. The most popular formulations, molecular weights and extraction methods are covered. The different crosslinking methods used in protein bioinks, the formulation with other polymeric systems or molecules of interest as well as the bioprinting settings are herein highlighted. The cell embedding procedures, the in vitro, in vivo, in situ studies and final applications are also discussed. Finally, we approach the development and optimization of bioinks from a sequential perspective, discussing the relevance of each parameter during the pre-processing, processing, and post-processing stages of technological development. Through this approach the present review expects to provide, in a sequential manner, helpful methodological guidelines for the development of novel bioinks.Veritati - Repositório Institucional da Universidade Católica PortuguesaVeiga, AnabelaSilva, Inês V.Duarte, Marta M.Oliveira, Ana L.2021-09-29T10:26:04Z2021-09-102021-09-10T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/35299eng1999-492310.3390/pharmaceutics1309144485115027367PMC847198434575521000699931900001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-12T01:34:36Zoai:repositorio.ucp.pt:10400.14/35299Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:28:39.939958Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Current trends on protein driven bioinks for 3D printing
title Current trends on protein driven bioinks for 3D printing
spellingShingle Current trends on protein driven bioinks for 3D printing
Veiga, Anabela
Bioink
Extrusion bioprinting
Protein-based
title_short Current trends on protein driven bioinks for 3D printing
title_full Current trends on protein driven bioinks for 3D printing
title_fullStr Current trends on protein driven bioinks for 3D printing
title_full_unstemmed Current trends on protein driven bioinks for 3D printing
title_sort Current trends on protein driven bioinks for 3D printing
author Veiga, Anabela
author_facet Veiga, Anabela
Silva, Inês V.
Duarte, Marta M.
Oliveira, Ana L.
author_role author
author2 Silva, Inês V.
Duarte, Marta M.
Oliveira, Ana L.
author2_role author
author
author
dc.contributor.none.fl_str_mv Veritati - Repositório Institucional da Universidade Católica Portuguesa
dc.contributor.author.fl_str_mv Veiga, Anabela
Silva, Inês V.
Duarte, Marta M.
Oliveira, Ana L.
dc.subject.por.fl_str_mv Bioink
Extrusion bioprinting
Protein-based
topic Bioink
Extrusion bioprinting
Protein-based
description In the last decade, three-dimensional (3D) extrusion bioprinting has been on the top trend for innovative technologies in the field of biomedical engineering. In particular, protein-based bioinks such as collagen, gelatin, silk fibroin, elastic, fibrin and protein complexes based on decellularized extracellular matrix (dECM) are receiving increasing attention. This current interest is the result of protein’s tunable properties, biocompatibility, environmentally friendly nature and possibility to provide cells with the adequate cues, mimicking the extracellular matrix’s function. In this review we describe the most relevant stages of the development of a protein-driven bioink. The most popular formulations, molecular weights and extraction methods are covered. The different crosslinking methods used in protein bioinks, the formulation with other polymeric systems or molecules of interest as well as the bioprinting settings are herein highlighted. The cell embedding procedures, the in vitro, in vivo, in situ studies and final applications are also discussed. Finally, we approach the development and optimization of bioinks from a sequential perspective, discussing the relevance of each parameter during the pre-processing, processing, and post-processing stages of technological development. Through this approach the present review expects to provide, in a sequential manner, helpful methodological guidelines for the development of novel bioinks.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-29T10:26:04Z
2021-09-10
2021-09-10T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.14/35299
url http://hdl.handle.net/10400.14/35299
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1999-4923
10.3390/pharmaceutics13091444
85115027367
PMC8471984
34575521
000699931900001
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