17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042006000200006 |
Resumo: | Sex hormones like 17ß-estradiol (ßES) and progesterone have shown rapid non-genomic vasodilator effects, which could be involved in the protection of cardiovascular system. However, the precise mechanism by which this effect occurs has not been elucidated yet, even if Ca2+ influx inhibition seems to be implicated. The aim of this study was to study the influence of ßES and progesterone on the L-type Ca2+ current measured by whole cell voltage-clamp in A7r5 cells. Voltage-operated Ca2+ currents were elicited by square-step voltage pulses and pharmacologically characterized as L-type currents by (-)-Bay K8644 (BAY) and nifedipine. Both ßES and progesterone (1-100 µM), rapidly and reversibly inhibited, in a concentration dependent manner, either non-stimulated or BAY-stimulated Ca2+ currents registered in A7r5 cells. These results suggest that ßES and progesterone inhibit L-type voltage-operated Ca2+ channels through a non-genomic pathway. Consequently, these hormones inhibit the Ca2+ entry into smooth muscle cells from rat aorta, an effect that can contribute for the protection of the cardiovascular system. |
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17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cellssex hormonessteroid non-genomic effectsL-type Ca2+ currents,patch-clampA7r5 cellsSex hormones like 17ß-estradiol (ßES) and progesterone have shown rapid non-genomic vasodilator effects, which could be involved in the protection of cardiovascular system. However, the precise mechanism by which this effect occurs has not been elucidated yet, even if Ca2+ influx inhibition seems to be implicated. The aim of this study was to study the influence of ßES and progesterone on the L-type Ca2+ current measured by whole cell voltage-clamp in A7r5 cells. Voltage-operated Ca2+ currents were elicited by square-step voltage pulses and pharmacologically characterized as L-type currents by (-)-Bay K8644 (BAY) and nifedipine. Both ßES and progesterone (1-100 µM), rapidly and reversibly inhibited, in a concentration dependent manner, either non-stimulated or BAY-stimulated Ca2+ currents registered in A7r5 cells. These results suggest that ßES and progesterone inhibit L-type voltage-operated Ca2+ channels through a non-genomic pathway. Consequently, these hormones inhibit the Ca2+ entry into smooth muscle cells from rat aorta, an effect that can contribute for the protection of the cardiovascular system.Sociedade Portuguesa de Electroquímica2006-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articletext/htmlhttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042006000200006Portugaliae Electrochimica Acta v.24 n.2 2006reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAPenghttp://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042006000200006Cairrão,E.Carvas,J.Santos-Silva,A.J.Alvarez,E.Verde,I.info:eu-repo/semantics/openAccess2024-02-06T17:06:48Zoai:scielo:S0872-19042006000200006Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T02:20:00.690083Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
title |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
spellingShingle |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells Cairrão,E. sex hormones steroid non-genomic effects L-type Ca2+ currents, patch-clamp A7r5 cells |
title_short |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
title_full |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
title_fullStr |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
title_full_unstemmed |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
title_sort |
17ß-Estradiol and progesterone inhibit l-type Ca2+ current of rat aorta smooth muscle cells |
author |
Cairrão,E. |
author_facet |
Cairrão,E. Carvas,J. Santos-Silva,A.J. Alvarez,E. Verde,I. |
author_role |
author |
author2 |
Carvas,J. Santos-Silva,A.J. Alvarez,E. Verde,I. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cairrão,E. Carvas,J. Santos-Silva,A.J. Alvarez,E. Verde,I. |
dc.subject.por.fl_str_mv |
sex hormones steroid non-genomic effects L-type Ca2+ currents, patch-clamp A7r5 cells |
topic |
sex hormones steroid non-genomic effects L-type Ca2+ currents, patch-clamp A7r5 cells |
description |
Sex hormones like 17ß-estradiol (ßES) and progesterone have shown rapid non-genomic vasodilator effects, which could be involved in the protection of cardiovascular system. However, the precise mechanism by which this effect occurs has not been elucidated yet, even if Ca2+ influx inhibition seems to be implicated. The aim of this study was to study the influence of ßES and progesterone on the L-type Ca2+ current measured by whole cell voltage-clamp in A7r5 cells. Voltage-operated Ca2+ currents were elicited by square-step voltage pulses and pharmacologically characterized as L-type currents by (-)-Bay K8644 (BAY) and nifedipine. Both ßES and progesterone (1-100 µM), rapidly and reversibly inhibited, in a concentration dependent manner, either non-stimulated or BAY-stimulated Ca2+ currents registered in A7r5 cells. These results suggest that ßES and progesterone inhibit L-type voltage-operated Ca2+ channels through a non-genomic pathway. Consequently, these hormones inhibit the Ca2+ entry into smooth muscle cells from rat aorta, an effect that can contribute for the protection of the cardiovascular system. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042006000200006 |
url |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042006000200006 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
http://scielo.pt/scielo.php?script=sci_arttext&pid=S0872-19042006000200006 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Electroquímica |
dc.source.none.fl_str_mv |
Portugaliae Electrochimica Acta v.24 n.2 2006 reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799137289423552512 |