Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria

Detalhes bibliográficos
Autor(a) principal: Louro, Ricardo O.
Data de Publicação: 2023
Outros Autores: Rusconi, Giovanni, Fonseca, Bruno M., Paquete, Catarina M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158517
Resumo: Funding Information: This work was funded by national funds through FCT–Fundação para a Ciência e a Tecnologia, I.P. (FCT), Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, project PTDC/BIA-BQM/4143/2021. The NMR data were acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT, project AAC 01/SAICT/2016. CMP acknowledges support of CA19123 COST ACTION PHOENIX. Publisher Copyright: © 2022 by the authors.
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spelling Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive BacteriaThe Case of OmcA from Shewanella oneidensis MR-1binding processesextracellular respirationindirect electron transfermutagenesisouter-membrane cytochromeShewanella oneidensis MR-1MicrobiologyMicrobiology (medical)VirologyFunding Information: This work was funded by national funds through FCT–Fundação para a Ciência e a Tecnologia, I.P. (FCT), Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, project PTDC/BIA-BQM/4143/2021. The NMR data were acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT, project AAC 01/SAICT/2016. CMP acknowledges support of CA19123 COST ACTION PHOENIX. Publisher Copyright: © 2022 by the authors.Multiheme cytochromes play a central role in extracellular electron transfer, a process that allows microorganisms to sustain their metabolism with external electron acceptors or donors. In Shewanella oneidensis MR-1, the decaheme cytochromes OmcA and MtrC show functional specificity for interaction with soluble and insoluble redox partners. In this work, the capacity of extracellular electron transfer by mutant variants of S. oneidensis MR-1 OmcA was investigated. The results show that amino acid mutations can affect protein stability and alter the redox properties of the protein, without affecting the ability to perform extracellular electron transfer to methyl orange dye or a poised electrode. The results also show that there is a good correlation between the reduction of the dye and the current generated at the electrode for most but not all mutants. This observation opens the door for investigations of the molecular mechanisms of interaction with different electron acceptors to tailor these surface exposed cytochromes towards specific bio-based applications.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNLouro, Ricardo O.Rusconi, GiovanniFonseca, Bruno M.Paquete, Catarina M.2023-09-30T22:18:24Z2023-012023-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158517eng2076-2607PURE: 72094502https://doi.org/10.3390/microorganisms11010079info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:40:56Zoai:run.unl.pt:10362/158517Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:10.880004Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
The Case of OmcA from Shewanella oneidensis MR-1
title Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
spellingShingle Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
Louro, Ricardo O.
binding processes
extracellular respiration
indirect electron transfer
mutagenesis
outer-membrane cytochrome
Shewanella oneidensis MR-1
Microbiology
Microbiology (medical)
Virology
title_short Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
title_full Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
title_fullStr Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
title_full_unstemmed Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
title_sort Deciphering Molecular Factors That Affect Electron Transfer at the Cell Surface of Electroactive Bacteria
author Louro, Ricardo O.
author_facet Louro, Ricardo O.
Rusconi, Giovanni
Fonseca, Bruno M.
Paquete, Catarina M.
author_role author
author2 Rusconi, Giovanni
Fonseca, Bruno M.
Paquete, Catarina M.
author2_role author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Louro, Ricardo O.
Rusconi, Giovanni
Fonseca, Bruno M.
Paquete, Catarina M.
dc.subject.por.fl_str_mv binding processes
extracellular respiration
indirect electron transfer
mutagenesis
outer-membrane cytochrome
Shewanella oneidensis MR-1
Microbiology
Microbiology (medical)
Virology
topic binding processes
extracellular respiration
indirect electron transfer
mutagenesis
outer-membrane cytochrome
Shewanella oneidensis MR-1
Microbiology
Microbiology (medical)
Virology
description Funding Information: This work was funded by national funds through FCT–Fundação para a Ciência e a Tecnologia, I.P. (FCT), Project MOSTMICRO-ITQB with refs UIDB/04612/2020 and UIDP/04612/2020, project PTDC/BIA-BQM/4143/2021. The NMR data were acquired at CERMAX, ITQB-NOVA, Oeiras, Portugal, with equipment funded by FCT, project AAC 01/SAICT/2016. CMP acknowledges support of CA19123 COST ACTION PHOENIX. Publisher Copyright: © 2022 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:18:24Z
2023-01
2023-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158517
url http://hdl.handle.net/10362/158517
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-2607
PURE: 72094502
https://doi.org/10.3390/microorganisms11010079
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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