Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)

Detalhes bibliográficos
Autor(a) principal: Jardim-Messeder, Douglas
Data de Publicação: 2023
Outros Autores: Caverzan, Andreia, Balbinott, Natalia, Menguer, Paloma K., Paiva, Ana L.S., Lemos, Moaciria, Cunha, Juliana R., Gaeta, Marcos L., Costa, Miguel, Zamocky, Marcel, Saibo, Nelson J.M., Silveira, Joaquim A.G., Margis, Rogério, Margis-Pinheiro, Márcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/158556
Resumo: Funding Information: This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/304583/2018-9), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Distrito Federal (FAPDF), INCT Plant-Stress Biotech (193.001.265/2017), Fundação para a Ciência e a Tecnologia (FCT) through the project GREEN-IT Bioresources for Sustainability R&D (Unit UIDB/04551/2020 and UIDP/04551/2020), Slovak Research and Development Agency (project APVV-20-0284), and Slovak Grant Agency (VEGA 2/0012/22). Publisher Copyright: © 2023 by the authors.
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spelling Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)abiotic stressascorbate peroxidasechloroplastdroughtphotosynthesisplant stress responsereactive oxygen speciesFood SciencePhysiologyBiochemistryMolecular BiologyClinical BiochemistryCell BiologyFunding Information: This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/304583/2018-9), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Distrito Federal (FAPDF), INCT Plant-Stress Biotech (193.001.265/2017), Fundação para a Ciência e a Tecnologia (FCT) through the project GREEN-IT Bioresources for Sustainability R&D (Unit UIDB/04551/2020 and UIDP/04551/2020), Slovak Research and Development Agency (project APVV-20-0284), and Slovak Grant Agency (VEGA 2/0012/22). Publisher Copyright: © 2023 by the authors.Chloroplast ascorbate peroxidases exert an important role in the maintenance of hydrogen peroxide levels in chloroplasts by using ascorbate as the specific electron donor. In this work, we performed a functional study of the stromal APX in rice (OsAPX7) and demonstrated that silencing of OsAPX7 did not impact plant growth, redox state, or photosynthesis parameters. Nevertheless, when subjected to drought stress, silenced plants (APX7i) show a higher capacity to maintain stomata aperture and photosynthesis performance, resulting in a higher tolerance when compared to non-transformed plants. RNA-seq analyses indicate that the silencing of OsAPX7 did not lead to changes in the global expression of genes related to reactive oxygen species metabolism. In addition, the drought-mediated induction of several genes related to the proteasome pathway and the down-regulation of genes related to nitrogen and carotenoid metabolism was impaired in APX7i plants. During drought stress, APX7i showed an up-regulation of genes encoding flavonoid and tyrosine metabolism enzymes and a down-regulation of genes related to phytohormones signal transduction and nicotinate and nicotinamide metabolism. Our results demonstrate that OsAPX7 might be involved in signaling transduction pathways related to drought stress response, contributing to the understanding of the physiological role of chloroplast APX isoforms in rice.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNJardim-Messeder, DouglasCaverzan, AndreiaBalbinott, NataliaMenguer, Paloma K.Paiva, Ana L.S.Lemos, MoaciriaCunha, Juliana R.Gaeta, Marcos L.Costa, MiguelZamocky, MarcelSaibo, Nelson J.M.Silveira, Joaquim A.G.Margis, RogérioMargis-Pinheiro, Márcia2023-09-30T22:22:43Z2023-022023-02-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/158556eng2076-3921PURE: 72716627https://doi.org/10.3390/antiox12020387info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:41:07Zoai:run.unl.pt:10362/158556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:57:12.975259Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
spellingShingle Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
Jardim-Messeder, Douglas
abiotic stress
ascorbate peroxidase
chloroplast
drought
photosynthesis
plant stress response
reactive oxygen species
Food Science
Physiology
Biochemistry
Molecular Biology
Clinical Biochemistry
Cell Biology
title_short Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_full Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_fullStr Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_full_unstemmed Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
title_sort Stromal Ascorbate Peroxidase (OsAPX7) Modulates Drought Stress Tolerance in Rice (Oryza sativa)
author Jardim-Messeder, Douglas
author_facet Jardim-Messeder, Douglas
Caverzan, Andreia
Balbinott, Natalia
Menguer, Paloma K.
Paiva, Ana L.S.
Lemos, Moaciria
Cunha, Juliana R.
Gaeta, Marcos L.
Costa, Miguel
Zamocky, Marcel
Saibo, Nelson J.M.
Silveira, Joaquim A.G.
Margis, Rogério
Margis-Pinheiro, Márcia
author_role author
author2 Caverzan, Andreia
Balbinott, Natalia
Menguer, Paloma K.
Paiva, Ana L.S.
Lemos, Moaciria
Cunha, Juliana R.
Gaeta, Marcos L.
Costa, Miguel
Zamocky, Marcel
Saibo, Nelson J.M.
Silveira, Joaquim A.G.
Margis, Rogério
Margis-Pinheiro, Márcia
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Jardim-Messeder, Douglas
Caverzan, Andreia
Balbinott, Natalia
Menguer, Paloma K.
Paiva, Ana L.S.
Lemos, Moaciria
Cunha, Juliana R.
Gaeta, Marcos L.
Costa, Miguel
Zamocky, Marcel
Saibo, Nelson J.M.
Silveira, Joaquim A.G.
Margis, Rogério
Margis-Pinheiro, Márcia
dc.subject.por.fl_str_mv abiotic stress
ascorbate peroxidase
chloroplast
drought
photosynthesis
plant stress response
reactive oxygen species
Food Science
Physiology
Biochemistry
Molecular Biology
Clinical Biochemistry
Cell Biology
topic abiotic stress
ascorbate peroxidase
chloroplast
drought
photosynthesis
plant stress response
reactive oxygen species
Food Science
Physiology
Biochemistry
Molecular Biology
Clinical Biochemistry
Cell Biology
description Funding Information: This work was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/304583/2018-9), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Amparo à Pesquisa do Distrito Federal (FAPDF), INCT Plant-Stress Biotech (193.001.265/2017), Fundação para a Ciência e a Tecnologia (FCT) through the project GREEN-IT Bioresources for Sustainability R&D (Unit UIDB/04551/2020 and UIDP/04551/2020), Slovak Research and Development Agency (project APVV-20-0284), and Slovak Grant Agency (VEGA 2/0012/22). Publisher Copyright: © 2023 by the authors.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-30T22:22:43Z
2023-02
2023-02-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/158556
url http://hdl.handle.net/10362/158556
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2076-3921
PURE: 72716627
https://doi.org/10.3390/antiox12020387
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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