Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering

Detalhes bibliográficos
Autor(a) principal: Tuzlakoglu, K.
Data de Publicação: 2005
Outros Autores: Bolgen, N., Salgado, A. J., Gomes, Manuela E., Piskin, Erhan, Reis, R. L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/20151
Resumo: One possible interesting way of designing a scaffold for bone tissue engineering is to base it on trying to mimic the biophysical structure of natural extracellular matrix (ECM). This work was developed in order to produce scaffolds for supporting bone cells. Nano and micro fiber combined scaffolds were originally produced from starch based biomaterials by means of a fiber bonding and a electrospinning, two step methodology. The cell culture studies with SaOs-2 human osteoblast-like cell line and rat bone marrow stromal cells demonstrated that presence of nanofibers influenced cell shape and cytoskeletal organization of the cells on the nano/micro combined scaffolds. Moreover, cell viability and Alkaline Phosphatase (ALP) activity for both cell types was found to be higher in nano/micro combined scaffolds than in control scaffolds based on fiber meshes without nanofibers. Consequently, the developed structures are believed have a great potential on the 3D organization and guidance of cells that is provided for engineering of 3-dimensional bone tissues.
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spelling Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineeringScience & TechnologyOne possible interesting way of designing a scaffold for bone tissue engineering is to base it on trying to mimic the biophysical structure of natural extracellular matrix (ECM). This work was developed in order to produce scaffolds for supporting bone cells. Nano and micro fiber combined scaffolds were originally produced from starch based biomaterials by means of a fiber bonding and a electrospinning, two step methodology. The cell culture studies with SaOs-2 human osteoblast-like cell line and rat bone marrow stromal cells demonstrated that presence of nanofibers influenced cell shape and cytoskeletal organization of the cells on the nano/micro combined scaffolds. Moreover, cell viability and Alkaline Phosphatase (ALP) activity for both cell types was found to be higher in nano/micro combined scaffolds than in control scaffolds based on fiber meshes without nanofibers. Consequently, the developed structures are believed have a great potential on the 3D organization and guidance of cells that is provided for engineering of 3-dimensional bone tissues.Springer ScienceUniversidade do MinhoTuzlakoglu, K.Bolgen, N.Salgado, A. J.Gomes, Manuela E.Piskin, ErhanReis, R. L.20052005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20151eng0957-453010.1007/s10856-005-4713-816362207info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:48Zoai:repositorium.sdum.uminho.pt:1822/20151Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:53:16.908189Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
title Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
spellingShingle Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
Tuzlakoglu, K.
Science & Technology
title_short Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
title_full Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
title_fullStr Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
title_full_unstemmed Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
title_sort Nano- and micro-fiber combined scaffolds : a new architecture for bone tissue engineering
author Tuzlakoglu, K.
author_facet Tuzlakoglu, K.
Bolgen, N.
Salgado, A. J.
Gomes, Manuela E.
Piskin, Erhan
Reis, R. L.
author_role author
author2 Bolgen, N.
Salgado, A. J.
Gomes, Manuela E.
Piskin, Erhan
Reis, R. L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Tuzlakoglu, K.
Bolgen, N.
Salgado, A. J.
Gomes, Manuela E.
Piskin, Erhan
Reis, R. L.
dc.subject.por.fl_str_mv Science & Technology
topic Science & Technology
description One possible interesting way of designing a scaffold for bone tissue engineering is to base it on trying to mimic the biophysical structure of natural extracellular matrix (ECM). This work was developed in order to produce scaffolds for supporting bone cells. Nano and micro fiber combined scaffolds were originally produced from starch based biomaterials by means of a fiber bonding and a electrospinning, two step methodology. The cell culture studies with SaOs-2 human osteoblast-like cell line and rat bone marrow stromal cells demonstrated that presence of nanofibers influenced cell shape and cytoskeletal organization of the cells on the nano/micro combined scaffolds. Moreover, cell viability and Alkaline Phosphatase (ALP) activity for both cell types was found to be higher in nano/micro combined scaffolds than in control scaffolds based on fiber meshes without nanofibers. Consequently, the developed structures are believed have a great potential on the 3D organization and guidance of cells that is provided for engineering of 3-dimensional bone tissues.
publishDate 2005
dc.date.none.fl_str_mv 2005
2005-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/20151
url http://hdl.handle.net/1822/20151
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0957-4530
10.1007/s10856-005-4713-8
16362207
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Science
publisher.none.fl_str_mv Springer Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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