The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/8325 |
Resumo: | The spindle assembly checkpoint monitors the status of kinetochore–microtubule (K-MT) attachments and delays anaphase onset until full metaphase alignment is achieved. Recently, the role of spindle assembly checkpoint proteins was expanded with the discovery that BubR1 and Bub1 are implicated in the regulation of K-MT attachments. One unsolved question is whether Bub3, known to form cell cycle constitutive complexes with both BubR1 and Bub1, is also required for proper chromosome-to-spindle attachments. Using RNA interference and high-resolution microscopy, we analyzed K-MT interactions in Bub3-depleted cells and compared them to those in Bub1- or BubR1-depleted cells. We found that Bub3 is essential for the establishment of correct K-MT attachments. In contrast to BubR1 depletion, which severely compromises chromosome attachment and alignment, we found Bub3 and Bub1 depletions to produce defective K-MT attachments that, however, still account for significant chromosome congression. After Aurora B inhibition, alignment defects become severer in Bub3- and Bub1-depleted cells, while partially rescued in BubR1-depleted cells, suggesting that Bub3 and Bub1 depletions perturb K-MT attachments distinctly from BubR1. Interestingly, misaligned chromosomes in Bub3- and Bub1-depleted cells were found to be predominantly bound in a side-on configuration. We propose that Bub3 promotes the formation of stable end-on bipolar attachments. |
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The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachmentsScience & TechnologyThe spindle assembly checkpoint monitors the status of kinetochore–microtubule (K-MT) attachments and delays anaphase onset until full metaphase alignment is achieved. Recently, the role of spindle assembly checkpoint proteins was expanded with the discovery that BubR1 and Bub1 are implicated in the regulation of K-MT attachments. One unsolved question is whether Bub3, known to form cell cycle constitutive complexes with both BubR1 and Bub1, is also required for proper chromosome-to-spindle attachments. Using RNA interference and high-resolution microscopy, we analyzed K-MT interactions in Bub3-depleted cells and compared them to those in Bub1- or BubR1-depleted cells. We found that Bub3 is essential for the establishment of correct K-MT attachments. In contrast to BubR1 depletion, which severely compromises chromosome attachment and alignment, we found Bub3 and Bub1 depletions to produce defective K-MT attachments that, however, still account for significant chromosome congression. After Aurora B inhibition, alignment defects become severer in Bub3- and Bub1-depleted cells, while partially rescued in BubR1-depleted cells, suggesting that Bub3 and Bub1 depletions perturb K-MT attachments distinctly from BubR1. Interestingly, misaligned chromosomes in Bub3- and Bub1-depleted cells were found to be predominantly bound in a side-on configuration. We propose that Bub3 promotes the formation of stable end-on bipolar attachments.Cooperativa de Ensino Superior Politécnico e Universitário (CESPU).Fundação para a Ciência e Tecnologia (FCT) - Grant POCTI/BCI/42341/2001.The American Society for Cell BiologyUniversidade do MinhoLogarinho, ElsaResende, TatianaTorres, C.Bousbaa, Hassan2008-042008-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfapplication/pdfhttp://hdl.handle.net/1822/8325eng"Molecular Biology of the Cell". ISSN 1939-4586. 19:4 (2008) 1798-1813.1939-458610.1091/mbc.E07-07-063318199686http://www.molbiolcell.org/cgi/reprint/19/4/1798?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=logarinho%2C+e&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCITinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:27:53Zoai:repositorium.sdum.uminho.pt:1822/8325Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:22:35.800910Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
title |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
spellingShingle |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments Logarinho, Elsa Science & Technology |
title_short |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
title_full |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
title_fullStr |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
title_full_unstemmed |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
title_sort |
The human spindle assembly checkpoint protein Bub3 is required for the establishment of efficient kinetochore-microtubule attachments |
author |
Logarinho, Elsa |
author_facet |
Logarinho, Elsa Resende, Tatiana Torres, C. Bousbaa, Hassan |
author_role |
author |
author2 |
Resende, Tatiana Torres, C. Bousbaa, Hassan |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Logarinho, Elsa Resende, Tatiana Torres, C. Bousbaa, Hassan |
dc.subject.por.fl_str_mv |
Science & Technology |
topic |
Science & Technology |
description |
The spindle assembly checkpoint monitors the status of kinetochore–microtubule (K-MT) attachments and delays anaphase onset until full metaphase alignment is achieved. Recently, the role of spindle assembly checkpoint proteins was expanded with the discovery that BubR1 and Bub1 are implicated in the regulation of K-MT attachments. One unsolved question is whether Bub3, known to form cell cycle constitutive complexes with both BubR1 and Bub1, is also required for proper chromosome-to-spindle attachments. Using RNA interference and high-resolution microscopy, we analyzed K-MT interactions in Bub3-depleted cells and compared them to those in Bub1- or BubR1-depleted cells. We found that Bub3 is essential for the establishment of correct K-MT attachments. In contrast to BubR1 depletion, which severely compromises chromosome attachment and alignment, we found Bub3 and Bub1 depletions to produce defective K-MT attachments that, however, still account for significant chromosome congression. After Aurora B inhibition, alignment defects become severer in Bub3- and Bub1-depleted cells, while partially rescued in BubR1-depleted cells, suggesting that Bub3 and Bub1 depletions perturb K-MT attachments distinctly from BubR1. Interestingly, misaligned chromosomes in Bub3- and Bub1-depleted cells were found to be predominantly bound in a side-on configuration. We propose that Bub3 promotes the formation of stable end-on bipolar attachments. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-04 2008-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/8325 |
url |
http://hdl.handle.net/1822/8325 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Molecular Biology of the Cell". ISSN 1939-4586. 19:4 (2008) 1798-1813. 1939-4586 10.1091/mbc.E07-07-0633 18199686 http://www.molbiolcell.org/cgi/reprint/19/4/1798?maxtoshow=&HITS=10&hits=10&RESULTFORMAT=&author1=logarinho%2C+e&andorexactfulltext=and&searchid=1&FIRSTINDEX=0&sortspec=relevance&resourcetype=HWCIT |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
The American Society for Cell Biology |
publisher.none.fl_str_mv |
The American Society for Cell Biology |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132697150357504 |