The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Maria João
Data de Publicação: 2022
Outros Autores: Jekő, József, Cziáky, Zoltán, Pereira, Catarina Guerreiro, L, Custódio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.1/17954
Resumo: This work explored the medicinal halophyte Frankenia laevis L. (sea heath) as a potential source of bioactive natural products. In this sense, methanol and dichloromethane extracts were prepared from aerial organs containing flowers, leaves and stems, and were profiled for their chemical composition using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). The extracts were evaluated for their in vitro antioxidant capacity using five complementary methods: enzyme inhibitory effects on enzymes related with neurodegeneration (acetyl (AChE) and butyrylcholinesterase (BuChE)), Type 2 diabetes (α-glucosidase and α-amylase), hyperpigmentation/food oxidation (tyrosinase), and cytotoxicity towards human hepatocarcinoma (HepG2) cells. Fifty-one molecules were identified in the extracts, including several derivatives of phenolic acids, lignans and flavonoids, monoterpenes, and hydroxylated derivatives of linoleic acid. The methanol extract was effective in DPPH and ABTS radical scavenging (EC50 = 0.25 and 0.65 mg/mL, respectively), copper chelation (EC50 = 0.78 mg/mL), and iron reduction (EC50 = 0.51 mg/mL) activities, whereas the dichloromethane extract had high iron chelating ability (EC50 = 0.76 mg/mL). Both extracts showed the capacity to inhibit α-glucosidase, especially the dichloromethane (EC50 = 0.52 mg/mL). This extract also exerted a significant selective cytotoxicity towards HepG2 cells (EC50 = 52.1 µg/mL, SI > 1.9). In conclusion, extracts from the aerial parts of sea heath were shown to be a promising source of natural products for pharmaceutical and/or food additive applications due to their high antioxidant, anti-diabetic, and cytotoxic properties.
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spelling The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cellsSalt-tolerant plantsFrankenia laevisPhenolicsMonoterpenesType 2 diabetesAnti-tumorThis work explored the medicinal halophyte Frankenia laevis L. (sea heath) as a potential source of bioactive natural products. In this sense, methanol and dichloromethane extracts were prepared from aerial organs containing flowers, leaves and stems, and were profiled for their chemical composition using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). The extracts were evaluated for their in vitro antioxidant capacity using five complementary methods: enzyme inhibitory effects on enzymes related with neurodegeneration (acetyl (AChE) and butyrylcholinesterase (BuChE)), Type 2 diabetes (α-glucosidase and α-amylase), hyperpigmentation/food oxidation (tyrosinase), and cytotoxicity towards human hepatocarcinoma (HepG2) cells. Fifty-one molecules were identified in the extracts, including several derivatives of phenolic acids, lignans and flavonoids, monoterpenes, and hydroxylated derivatives of linoleic acid. The methanol extract was effective in DPPH and ABTS radical scavenging (EC50 = 0.25 and 0.65 mg/mL, respectively), copper chelation (EC50 = 0.78 mg/mL), and iron reduction (EC50 = 0.51 mg/mL) activities, whereas the dichloromethane extract had high iron chelating ability (EC50 = 0.76 mg/mL). Both extracts showed the capacity to inhibit α-glucosidase, especially the dichloromethane (EC50 = 0.52 mg/mL). This extract also exerted a significant selective cytotoxicity towards HepG2 cells (EC50 = 52.1 µg/mL, SI > 1.9). In conclusion, extracts from the aerial parts of sea heath were shown to be a promising source of natural products for pharmaceutical and/or food additive applications due to their high antioxidant, anti-diabetic, and cytotoxic properties.MDPISapientiaRodrigues, Maria JoãoJekő, JózsefCziáky, ZoltánPereira, Catarina GuerreiroL, Custódio2022-07-11T13:07:49Z2022-05-192022-05-27T13:36:50Z2022-05-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.1/17954engPlants 11 (10): 1353 (2022)10.3390/plants111013532223-7747info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-24T10:30:05Zoai:sapientia.ualg.pt:10400.1/17954Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:07:43.349034Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
title The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
spellingShingle The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
Rodrigues, Maria João
Salt-tolerant plants
Frankenia laevis
Phenolics
Monoterpenes
Type 2 diabetes
Anti-tumor
title_short The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
title_full The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
title_fullStr The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
title_full_unstemmed The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
title_sort The medicinal halophyte frankenia laevis l. (Sea Heath) has in vitro antioxidant activity, α-glucosidase inhibition, and cytotoxicity towards hepatocarcinoma cells
author Rodrigues, Maria João
author_facet Rodrigues, Maria João
Jekő, József
Cziáky, Zoltán
Pereira, Catarina Guerreiro
L, Custódio
author_role author
author2 Jekő, József
Cziáky, Zoltán
Pereira, Catarina Guerreiro
L, Custódio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Sapientia
dc.contributor.author.fl_str_mv Rodrigues, Maria João
Jekő, József
Cziáky, Zoltán
Pereira, Catarina Guerreiro
L, Custódio
dc.subject.por.fl_str_mv Salt-tolerant plants
Frankenia laevis
Phenolics
Monoterpenes
Type 2 diabetes
Anti-tumor
topic Salt-tolerant plants
Frankenia laevis
Phenolics
Monoterpenes
Type 2 diabetes
Anti-tumor
description This work explored the medicinal halophyte Frankenia laevis L. (sea heath) as a potential source of bioactive natural products. In this sense, methanol and dichloromethane extracts were prepared from aerial organs containing flowers, leaves and stems, and were profiled for their chemical composition using high-performance liquid chromatography coupled with electrospray ionization mass spectrometry (HPLC-ESI-MS/MS). The extracts were evaluated for their in vitro antioxidant capacity using five complementary methods: enzyme inhibitory effects on enzymes related with neurodegeneration (acetyl (AChE) and butyrylcholinesterase (BuChE)), Type 2 diabetes (α-glucosidase and α-amylase), hyperpigmentation/food oxidation (tyrosinase), and cytotoxicity towards human hepatocarcinoma (HepG2) cells. Fifty-one molecules were identified in the extracts, including several derivatives of phenolic acids, lignans and flavonoids, monoterpenes, and hydroxylated derivatives of linoleic acid. The methanol extract was effective in DPPH and ABTS radical scavenging (EC50 = 0.25 and 0.65 mg/mL, respectively), copper chelation (EC50 = 0.78 mg/mL), and iron reduction (EC50 = 0.51 mg/mL) activities, whereas the dichloromethane extract had high iron chelating ability (EC50 = 0.76 mg/mL). Both extracts showed the capacity to inhibit α-glucosidase, especially the dichloromethane (EC50 = 0.52 mg/mL). This extract also exerted a significant selective cytotoxicity towards HepG2 cells (EC50 = 52.1 µg/mL, SI > 1.9). In conclusion, extracts from the aerial parts of sea heath were shown to be a promising source of natural products for pharmaceutical and/or food additive applications due to their high antioxidant, anti-diabetic, and cytotoxic properties.
publishDate 2022
dc.date.none.fl_str_mv 2022-07-11T13:07:49Z
2022-05-19
2022-05-27T13:36:50Z
2022-05-19T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.1/17954
url http://hdl.handle.net/10400.1/17954
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Plants 11 (10): 1353 (2022)
10.3390/plants11101353
2223-7747
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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