Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/1822/33628 |
Resumo: | Publicado em "Innate Immunity". ISSN 1753-4259, vol. 18, n.º 5 (2012), p. 700-708 |
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Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequenceChronic hepatitisCirrhosisHepatocarcinomaTLR2TLR4Science & TechnologyPublicado em "Innate Immunity". ISSN 1753-4259, vol. 18, n.º 5 (2012), p. 700-708We evaluated expression of TLR2, TLR4 and proinflammatory genes [NF-?B, TNF-a, cyclooxygenase-2 (COX-2)] in liver samples of patients in different stages of liver disease. Fifteen patients with unexplained transaminases elevation (reference group), 22 with viral chronic hepatitis (hepatitis group), 14 with virus-induced severe fibrosis/cirrhosis (cirrhosis group) and 10 with hepatocarcinoma (hepatocarcinoma group) were consecutively included in the study. Quantification of TLR2, TLR4, NF-?B, TNF-a and COX-2 mRNA was done by real-time RT-PCR and TLR2 and TLR4 protein expression was evaluated by immunohistochemistry. Compared with reference, TLR2 and TLR4 mRNA was increased in hepatitis (TLR2: 2.66?±?0.69; TLR4: 3.11?±?0.79; P?<?0.05) and cirrhosis (TLR2: 2.14?±?0.5; TLR4: 1.74?±?0.27; P?<?0.05) and decreased in hepatocarcinoma (TLR2: 0.48?±?0.15; TLR4: 0.54?±?0.10; P?<?0.05). This associated with increased TNF-a and COX-2 mRNA in hepatitis (TNF-a: 3.24?±?0.79; COX-2: 2.47?±?0.36; P?<?0.05) and cirrhosis (TNF-a: 1.73?±?0.28; COX-2: 1.8?±?0.35, P?<?0.05), whereas NF-?B mRNA was increased in hepatitis (2.42?±?0.31; P?<?0.05) and unchanged in cirrhosis (1.34?±?0.17; P?=?0.3). Hepatocarcinoma presented increased COX-2 mRNA (1.63?±?0.15; P?<?0.05) and maintained (at decreased levels) mRNA of NF-?B (0.52?±?0.12) and TNF-a (0.52?±?0.12; P?<?0.05, all genes). Immunohistochemistry confirmed increased expression of TLR2 and TLR4 in hepatitis and cirrhosis and maintained expression in hepatocarcinoma. Upregulation of TLR2, TLR4 and their proinflammatory mediators is associated with virus-induced hepatic IFC sequence.SAGE PublicationsUniversidade do MinhoSoares, João BrunoNunes, Pedro PimentelAfonso, LuísRolanda, CarlaLopes, PaulaRoncon-Albuquerque, R.Gonçalves, NádiaCarvalho, Inês BoalPardal, FernandoLopes, SusanaMacedo,GuilhermeHenrique, RuiDias, Rui MoreiraGonçalves, RaquelRibeiro, Mário DinisMoreira, Adelino F. Leite20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/33628eng1753-425910.1177/175342591243676222330637http://ini.sagepub.com/content/18/5/700.abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:03Zoai:repositorium.sdum.uminho.pt:1822/33628Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:06:16.268392Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| title |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| spellingShingle |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence Soares, João Bruno Chronic hepatitis Cirrhosis Hepatocarcinoma TLR2 TLR4 Science & Technology |
| title_short |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| title_full |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| title_fullStr |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| title_full_unstemmed |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| title_sort |
Increased hepatic expression of TLR2 and TLR4 in the hepatic inflammation-fibrosis-carcinoma sequence |
| author |
Soares, João Bruno |
| author_facet |
Soares, João Bruno Nunes, Pedro Pimentel Afonso, Luís Rolanda, Carla Lopes, Paula Roncon-Albuquerque, R. Gonçalves, Nádia Carvalho, Inês Boal Pardal, Fernando Lopes, Susana Macedo,Guilherme Henrique, Rui Dias, Rui Moreira Gonçalves, Raquel Ribeiro, Mário Dinis Moreira, Adelino F. Leite |
| author_role |
author |
| author2 |
Nunes, Pedro Pimentel Afonso, Luís Rolanda, Carla Lopes, Paula Roncon-Albuquerque, R. Gonçalves, Nádia Carvalho, Inês Boal Pardal, Fernando Lopes, Susana Macedo,Guilherme Henrique, Rui Dias, Rui Moreira Gonçalves, Raquel Ribeiro, Mário Dinis Moreira, Adelino F. Leite |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Universidade do Minho |
| dc.contributor.author.fl_str_mv |
Soares, João Bruno Nunes, Pedro Pimentel Afonso, Luís Rolanda, Carla Lopes, Paula Roncon-Albuquerque, R. Gonçalves, Nádia Carvalho, Inês Boal Pardal, Fernando Lopes, Susana Macedo,Guilherme Henrique, Rui Dias, Rui Moreira Gonçalves, Raquel Ribeiro, Mário Dinis Moreira, Adelino F. Leite |
| dc.subject.por.fl_str_mv |
Chronic hepatitis Cirrhosis Hepatocarcinoma TLR2 TLR4 Science & Technology |
| topic |
Chronic hepatitis Cirrhosis Hepatocarcinoma TLR2 TLR4 Science & Technology |
| description |
Publicado em "Innate Immunity". ISSN 1753-4259, vol. 18, n.º 5 (2012), p. 700-708 |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 2012-01-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/33628 |
| url |
http://hdl.handle.net/1822/33628 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1753-4259 10.1177/1753425912436762 22330637 http://ini.sagepub.com/content/18/5/700.abstract |
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info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
SAGE Publications |
| publisher.none.fl_str_mv |
SAGE Publications |
| dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132477441179648 |