Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice.
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/26428 |
Resumo: | Cryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in immunocompromised host. Immunoregulatory T cells play a central role in the regulatory network of the host. Here, we proposed to characterize the populations of immune cells during infection and reinfection with C. parvum. Four-week-old BALB/C mice were inoculated with oocysts of C. parvum at days 0 and 22. Fecal and blood samples, spleens, and small intestines were collected for analysis. Peripheral blood and spleen cell populations were characterized by flow cytometry. After infection (days 0 to 21), mice presented higher values of neutrophils, eosinophils, NK cells and CD4(+)CD25(high) T cells in peripheral blood. After reinfection, this upward trend continued in the following days for all four populations in infected mice. At day 35, infected mice presented similar values to the control group, except for CD4(+)CD25(high) T cells, which remained higher in infected mice. A possible correlation between alterations in blood and spleen cell populations was also studied, but no consistent association could be established. Small intestine sections were screened for intracellular stages of the parasite but no evidence of pathology was observed. Here, we report information which may be important for the understanding of the specific cell-mediated response in immunocompetent mice to C. parvum infection. Although some questions remain unanswered and complementary studies are needed, our results are expected to contribute to a better understanding of innate and Treg cells role in the clearance process of this parasite. |
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Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice.CryptosporidiumREGULATORY T-CELLSPOPULATIONSflow cytometryIMMUNE-RESPONSESPORTUGALimmunophenotypeTRANSMISSIONT-lymphocytesWATERSCID MICEHIVB-lymphocytesPCR-RFLP ANALYSISDEFICIENT MICEParasitologyInfectious DiseasesSDG 3 - Good Health and Well-beingCryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in immunocompromised host. Immunoregulatory T cells play a central role in the regulatory network of the host. Here, we proposed to characterize the populations of immune cells during infection and reinfection with C. parvum. Four-week-old BALB/C mice were inoculated with oocysts of C. parvum at days 0 and 22. Fecal and blood samples, spleens, and small intestines were collected for analysis. Peripheral blood and spleen cell populations were characterized by flow cytometry. After infection (days 0 to 21), mice presented higher values of neutrophils, eosinophils, NK cells and CD4(+)CD25(high) T cells in peripheral blood. After reinfection, this upward trend continued in the following days for all four populations in infected mice. At day 35, infected mice presented similar values to the control group, except for CD4(+)CD25(high) T cells, which remained higher in infected mice. A possible correlation between alterations in blood and spleen cell populations was also studied, but no consistent association could be established. Small intestine sections were screened for intracellular stages of the parasite but no evidence of pathology was observed. Here, we report information which may be important for the understanding of the specific cell-mediated response in immunocompetent mice to C. parvum infection. Although some questions remain unanswered and complementary studies are needed, our results are expected to contribute to a better understanding of innate and Treg cells role in the clearance process of this parasite.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)Instituto de Higiene e Medicina Tropical (IHMT)Centro de Malária e outras Doenças Tropicais (CMDT)RUNCodices, VeraMartins, CatarinaNovo, Carlos Manuel MendesPinho, MSousa, Bruno Cecílio deLopes, ÂngelaBorrego, Luís Miguel NabaisMatos, Olga Maria Guerreiro de2017-12-07T23:01:20Z2013-01-012013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/26428eng1230-2821PURE: 349036https://doi.org/10.2478/s11686-013-0113-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:13:56Zoai:run.unl.pt:10362/26428Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:28:29.609786Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
title |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
spellingShingle |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. Codices, Vera Cryptosporidium REGULATORY T-CELLS POPULATIONS flow cytometry IMMUNE-RESPONSES PORTUGAL immunophenotype TRANSMISSION T-lymphocytes WATER SCID MICE HIV B-lymphocytes PCR-RFLP ANALYSIS DEFICIENT MICE Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
title_short |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
title_full |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
title_fullStr |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
title_full_unstemmed |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
title_sort |
Cell phenotypic change due to Cryptosporidium parvum infection in immunocompetent mice. |
author |
Codices, Vera |
author_facet |
Codices, Vera Martins, Catarina Novo, Carlos Manuel Mendes Pinho, M Sousa, Bruno Cecílio de Lopes, Ângela Borrego, Luís Miguel Nabais Matos, Olga Maria Guerreiro de |
author_role |
author |
author2 |
Martins, Catarina Novo, Carlos Manuel Mendes Pinho, M Sousa, Bruno Cecílio de Lopes, Ângela Borrego, Luís Miguel Nabais Matos, Olga Maria Guerreiro de |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) Instituto de Higiene e Medicina Tropical (IHMT) Centro de Malária e outras Doenças Tropicais (CMDT) RUN |
dc.contributor.author.fl_str_mv |
Codices, Vera Martins, Catarina Novo, Carlos Manuel Mendes Pinho, M Sousa, Bruno Cecílio de Lopes, Ângela Borrego, Luís Miguel Nabais Matos, Olga Maria Guerreiro de |
dc.subject.por.fl_str_mv |
Cryptosporidium REGULATORY T-CELLS POPULATIONS flow cytometry IMMUNE-RESPONSES PORTUGAL immunophenotype TRANSMISSION T-lymphocytes WATER SCID MICE HIV B-lymphocytes PCR-RFLP ANALYSIS DEFICIENT MICE Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
topic |
Cryptosporidium REGULATORY T-CELLS POPULATIONS flow cytometry IMMUNE-RESPONSES PORTUGAL immunophenotype TRANSMISSION T-lymphocytes WATER SCID MICE HIV B-lymphocytes PCR-RFLP ANALYSIS DEFICIENT MICE Parasitology Infectious Diseases SDG 3 - Good Health and Well-being |
description |
Cryptosporidium parvum is an intracellular parasite causing enteritis which can become life-threatening in immunocompromised host. Immunoregulatory T cells play a central role in the regulatory network of the host. Here, we proposed to characterize the populations of immune cells during infection and reinfection with C. parvum. Four-week-old BALB/C mice were inoculated with oocysts of C. parvum at days 0 and 22. Fecal and blood samples, spleens, and small intestines were collected for analysis. Peripheral blood and spleen cell populations were characterized by flow cytometry. After infection (days 0 to 21), mice presented higher values of neutrophils, eosinophils, NK cells and CD4(+)CD25(high) T cells in peripheral blood. After reinfection, this upward trend continued in the following days for all four populations in infected mice. At day 35, infected mice presented similar values to the control group, except for CD4(+)CD25(high) T cells, which remained higher in infected mice. A possible correlation between alterations in blood and spleen cell populations was also studied, but no consistent association could be established. Small intestine sections were screened for intracellular stages of the parasite but no evidence of pathology was observed. Here, we report information which may be important for the understanding of the specific cell-mediated response in immunocompetent mice to C. parvum infection. Although some questions remain unanswered and complementary studies are needed, our results are expected to contribute to a better understanding of innate and Treg cells role in the clearance process of this parasite. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 2013-01-01T00:00:00Z 2017-12-07T23:01:20Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/26428 |
url |
http://hdl.handle.net/10362/26428 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1230-2821 PURE: 349036 https://doi.org/10.2478/s11686-013-0113-2 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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