Influence of the electrolyte salt concentration on DNA detection with graphene transistors
Autor(a) principal: | |
---|---|
Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/72255 |
Resumo: | Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that <i>λ<sub>D</sub></i> is not the main factor governing the effective nanoscale screening environment. We observed that the longer <i>λ<sub>D</sub></i> was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity. |
id |
RCAP_a5028448686b1e5ef8d9be52ce6259a0 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/72255 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Influence of the electrolyte salt concentration on DNA detection with graphene transistorsliquid gategrapheneDebye lengthphosphate buffer (PB)phosphate buffer saline (PBS)saltsDNACiências Naturais::Ciências FísicasScience & TechnologyLiquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that <i>λ<sub>D</sub></i> is not the main factor governing the effective nanoscale screening environment. We observed that the longer <i>λ<sub>D</sub></i> was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity.This research is supported by PORTGRAPHE-Control of Port and DouroWines authenticity using graphene DNA sensors project co-funded by FCT (PTDC/BIA-MOL/31069/2017) and the ERDF through COMPETE2020 (POCI-01-0145-FEDER-031069).Multidisciplinary Digital Publishing InstituteUniversidade do MinhoPurwidyantri, AgnesDomingues, TelmaBorme, JérômeGuerreiro, Joana RafaelaIpatov, AndreyAbreu, Catarina M.Martins, MarcoAlpuim, P.Prado, Marta20212021-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/72255engPurwidyantri, A.; Domingues, T.; Borme, J.; Guerreiro, J.R.; Ipatov, A.; Abreu, C.M.; Martins, M.; Alpuim, P.; Prado, M. Influence of the Electrolyte Salt Concentration on DNA Detection with Graphene Transistors. Biosensors 2021, 11, 24. https://doi.org/10.3390/bios110100242079-637410.3390/bios1101002433477344https://www.mdpi.com/2079-6374/11/1/24info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:53:55Zoai:repositorium.sdum.uminho.pt:1822/72255Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:23.141765Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
title |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
spellingShingle |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors Purwidyantri, Agnes liquid gate graphene Debye length phosphate buffer (PB) phosphate buffer saline (PBS) salts DNA Ciências Naturais::Ciências Físicas Science & Technology |
title_short |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
title_full |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
title_fullStr |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
title_full_unstemmed |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
title_sort |
Influence of the electrolyte salt concentration on DNA detection with graphene transistors |
author |
Purwidyantri, Agnes |
author_facet |
Purwidyantri, Agnes Domingues, Telma Borme, Jérôme Guerreiro, Joana Rafaela Ipatov, Andrey Abreu, Catarina M. Martins, Marco Alpuim, P. Prado, Marta |
author_role |
author |
author2 |
Domingues, Telma Borme, Jérôme Guerreiro, Joana Rafaela Ipatov, Andrey Abreu, Catarina M. Martins, Marco Alpuim, P. Prado, Marta |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Purwidyantri, Agnes Domingues, Telma Borme, Jérôme Guerreiro, Joana Rafaela Ipatov, Andrey Abreu, Catarina M. Martins, Marco Alpuim, P. Prado, Marta |
dc.subject.por.fl_str_mv |
liquid gate graphene Debye length phosphate buffer (PB) phosphate buffer saline (PBS) salts DNA Ciências Naturais::Ciências Físicas Science & Technology |
topic |
liquid gate graphene Debye length phosphate buffer (PB) phosphate buffer saline (PBS) salts DNA Ciências Naturais::Ciências Físicas Science & Technology |
description |
Liquid-gated Graphene Field-Effect Transistors (GFET) are ultrasensitive bio-detection platforms carrying out the graphene’s exceptional intrinsic functionalities. Buffer and dilution factor are prevalent strategies towards the optimum performance of the GFETs. However, beyond the Debye length (λD), the role of the graphene-electrolytes’ ionic species interactions on the DNA behavior at the nanoscale interface is complicated. We studied the characteristics of the GFETs under different ionic strength, pH, and electrolyte type, e.g., phosphate buffer (PB), and phosphate buffer saline (PBS), in an automatic portable built-in system. The electrostatic gating and charge transfer phenomena were inferred from the field-effect measurements of the Dirac point position in single-layer graphene (SLG) transistors transfer curves. Results denote that <i>λ<sub>D</sub></i> is not the main factor governing the effective nanoscale screening environment. We observed that the longer <i>λ<sub>D</sub></i> was not the determining characteristic for sensitivity increment and limit of detection (LoD) as demonstrated by different types and ionic strengths of measuring buffers. In the DNA hybridization study, our findings show the role of the additional salts present in PBS, as compared to PB, in increasing graphene electron mobility, electrostatic shielding, intermolecular forces and DNA adsorption kinetics leading to an improved sensitivity. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021 2021-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/72255 |
url |
http://hdl.handle.net/1822/72255 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Purwidyantri, A.; Domingues, T.; Borme, J.; Guerreiro, J.R.; Ipatov, A.; Abreu, C.M.; Martins, M.; Alpuim, P.; Prado, M. Influence of the Electrolyte Salt Concentration on DNA Detection with Graphene Transistors. Biosensors 2021, 11, 24. https://doi.org/10.3390/bios11010024 2079-6374 10.3390/bios11010024 33477344 https://www.mdpi.com/2079-6374/11/1/24 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799132180224409600 |