Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains

Detalhes bibliográficos
Autor(a) principal: Oliveira, Hugo
Data de Publicação: 2017
Outros Autores: Costa, Ana Rita, Konstantinidis, Nico, Ferreira, A., Akturk, Ergun, Sillankorva, Sanna, Nemec, Alexander, Shneider, Mikhail, Dötsch, Andreas, Azeredo, Joana
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/51511
Resumo: Bacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with a diversified group of bacterial hosts. Studying phage-host interactions is important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which exhibited a narrow host range and similar morphological features (podoviruses with short tails of 9-12 nm and isometric heads of 50-60 nm). Notably, the alignment of the newly sequenced phage genomes (40-41 kb of DNA length) and all Acinetobacter podoviruses deposited in Genbank has shown high synteny, regardless of the date and source of isolation that spans from America to Europe and Asia. Interestingly, the C-terminal pectate lyase domain of these phage tail fibers is often the only difference found among these viral genomes, demonstrating a very specific genomic variation during the course of their evolution. We proved that the pectate lyase domain is responsible for phage depolymerase activity and binding to specific Acinetobacter bacterial capsules. We discuss how this mechanism of phage-host co-evolution impacts the tail specificity apparatus of Acinetobacter podoviruses.
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spelling Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domainsAcinetobacter calcoaceticus-Acinetobacter baumannii complexbacteriophagecomparative genomicsdepolymerasecapsuleScience & TechnologyBacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with a diversified group of bacterial hosts. Studying phage-host interactions is important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which exhibited a narrow host range and similar morphological features (podoviruses with short tails of 9-12 nm and isometric heads of 50-60 nm). Notably, the alignment of the newly sequenced phage genomes (40-41 kb of DNA length) and all Acinetobacter podoviruses deposited in Genbank has shown high synteny, regardless of the date and source of isolation that spans from America to Europe and Asia. Interestingly, the C-terminal pectate lyase domain of these phage tail fibers is often the only difference found among these viral genomes, demonstrating a very specific genomic variation during the course of their evolution. We proved that the pectate lyase domain is responsible for phage depolymerase activity and binding to specific Acinetobacter bacterial capsules. We discuss how this mechanism of phage-host co-evolution impacts the tail specificity apparatus of Acinetobacter podoviruses.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit, COMPETE 2020 (POCI01–0145-FEDER-006684) and the Project PTDC/BBB-BSS/6471/2014 (POCI-01–0145-FEDER-016678). This work was also supported by BioTecNorte operation (NORTE-01–0145FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte. We acknowledge Dr. Lenie Dijkshoorn (Leiden Medical Center) for the provision of some strains (LUH or RUH designations). AFM was performed at i3s- Instituto de Investigação e Inovação para a Sa ude at the Biointerfaces and Nanotechnology platform. SS is an FCT Investigator (IF/01413/ 2013). HO and ARC acknowledge FCT for grants SFRH/BPD/ 111653/2015 and SFRH/BPD/94648/2013 respectively. The authors declare that they have no competing financial interests.info:eu-repo/semantics/publishedVersionWiley-BlackwellUniversidade do MinhoOliveira, HugoCosta, Ana RitaKonstantinidis, NicoFerreira, A.Akturk, ErgunSillankorva, SannaNemec, AlexanderShneider, MikhailDötsch, AndreasAzeredo, Joana2017-122017-12-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/51511engOliveira, Hugo; Costa, Ana Rita; Konstantinidis, Nico; Ferreira, A.; Akturk, Ergun; Sillankorva, Sanna; Nemec, Alexander; Shneider, Mikhail; Dötsch, Andreas; Azeredo, Joana, Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains. Environmental Microbiology, 19(12), 5060-5077, 20171462-29121462-292010.1111/1462-2920.1397029076652http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:04:20Zoai:repositorium.sdum.uminho.pt:1822/51511Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:54:37.812922Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
title Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
spellingShingle Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
Oliveira, Hugo
Acinetobacter calcoaceticus-Acinetobacter baumannii complex
bacteriophage
comparative genomics
depolymerase
capsule
Science & Technology
title_short Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
title_full Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
title_fullStr Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
title_full_unstemmed Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
title_sort Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains
author Oliveira, Hugo
author_facet Oliveira, Hugo
Costa, Ana Rita
Konstantinidis, Nico
Ferreira, A.
Akturk, Ergun
Sillankorva, Sanna
Nemec, Alexander
Shneider, Mikhail
Dötsch, Andreas
Azeredo, Joana
author_role author
author2 Costa, Ana Rita
Konstantinidis, Nico
Ferreira, A.
Akturk, Ergun
Sillankorva, Sanna
Nemec, Alexander
Shneider, Mikhail
Dötsch, Andreas
Azeredo, Joana
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Oliveira, Hugo
Costa, Ana Rita
Konstantinidis, Nico
Ferreira, A.
Akturk, Ergun
Sillankorva, Sanna
Nemec, Alexander
Shneider, Mikhail
Dötsch, Andreas
Azeredo, Joana
dc.subject.por.fl_str_mv Acinetobacter calcoaceticus-Acinetobacter baumannii complex
bacteriophage
comparative genomics
depolymerase
capsule
Science & Technology
topic Acinetobacter calcoaceticus-Acinetobacter baumannii complex
bacteriophage
comparative genomics
depolymerase
capsule
Science & Technology
description Bacteriophages are ubiquitous in nature and represent a vast repository of genetic diversity, which is driven by the endless coevolution cycle with a diversified group of bacterial hosts. Studying phage-host interactions is important to gain novel insights into their dynamic adaptation. In this study, we isolated 12 phages infecting species of the Acinetobacter baumannii-Acinetobacter calcoaceticus complex which exhibited a narrow host range and similar morphological features (podoviruses with short tails of 9-12 nm and isometric heads of 50-60 nm). Notably, the alignment of the newly sequenced phage genomes (40-41 kb of DNA length) and all Acinetobacter podoviruses deposited in Genbank has shown high synteny, regardless of the date and source of isolation that spans from America to Europe and Asia. Interestingly, the C-terminal pectate lyase domain of these phage tail fibers is often the only difference found among these viral genomes, demonstrating a very specific genomic variation during the course of their evolution. We proved that the pectate lyase domain is responsible for phage depolymerase activity and binding to specific Acinetobacter bacterial capsules. We discuss how this mechanism of phage-host co-evolution impacts the tail specificity apparatus of Acinetobacter podoviruses.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
2017-12-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/51511
url http://hdl.handle.net/1822/51511
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Oliveira, Hugo; Costa, Ana Rita; Konstantinidis, Nico; Ferreira, A.; Akturk, Ergun; Sillankorva, Sanna; Nemec, Alexander; Shneider, Mikhail; Dötsch, Andreas; Azeredo, Joana, Ability of phages to infect Acinetobacter calcoaceticus-Acinetobacter baumannii complex species through acquisition of different pectate lyase depolymerase domains. Environmental Microbiology, 19(12), 5060-5077, 2017
1462-2912
1462-2920
10.1111/1462-2920.13970
29076652
http://onlinelibrary.wiley.com/journal/10.1111/(ISSN)1462-2920
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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