Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1

Detalhes bibliográficos
Autor(a) principal: Coelho, Dina S.
Data de Publicação: 2014
Outros Autores: Gaspar, Catarina J., Domingos, Pedro M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/92249
Resumo: WOS:000340742100110
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spelling Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1Agricultural and Biological Sciences(all)Biochemistry, Genetics and Molecular Biology(all)Medicine(all)WOS:000340742100110The Unfolded Protein Response is a homeostatic mechanism that permits eukaryotic cells to cope with Endoplasmic Reticulum (ER) stress caused by excessive accumulation of misfolded proteins in the ER lumen. The more conserved branch of the UPR relies on an ER transmembrane enzyme, Ire1, which, upon ER stress, promotes the unconventional splicing of a small intron from the mRNA encoding the transcription factor Xbp1. In mammals, two specific regions (the hydrophobic region 2 - HR2 - and the C-terminal translational pausing site) present in the Xbp1unspliced protein mediate the recruitment of the Xbp1 mRNA-ribosome-nascent chain complex to the ER membrane, so that Xbp1 mRNA can be spliced by Ire1. Here, we generated a Drosophila Xbp1 deletion mutant (Excision101 ) lacking both HR2 and C-terminal region, but not the Ire1 splicing site. We show that Ire1-dependent splicing of Xbp1 mRNA is reduced, but not abolished in Excision101. Our results suggest the existence of additional mechanisms for ER membrane targeting of Xbp1 mRNA that are independent of the Cterminal domain of Drosophila Xbp1unspliced.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNCoelho, Dina S.Gaspar, Catarina J.Domingos, Pedro M.2020-02-05T23:22:29Z2014-08-192014-08-19T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/92249eng1932-6203PURE: 1729799https://doi.org/10.1371/journal.pone.0105588info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:41:03Zoai:run.unl.pt:10362/92249Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:37:28.873527Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
title Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
spellingShingle Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
Coelho, Dina S.
Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)
title_short Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
title_full Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
title_fullStr Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
title_full_unstemmed Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
title_sort Ire1 mediated mRNA splicing in a C-terminus deletion mutant of drosophila Xbp1
author Coelho, Dina S.
author_facet Coelho, Dina S.
Gaspar, Catarina J.
Domingos, Pedro M.
author_role author
author2 Gaspar, Catarina J.
Domingos, Pedro M.
author2_role author
author
dc.contributor.none.fl_str_mv Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Coelho, Dina S.
Gaspar, Catarina J.
Domingos, Pedro M.
dc.subject.por.fl_str_mv Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)
topic Agricultural and Biological Sciences(all)
Biochemistry, Genetics and Molecular Biology(all)
Medicine(all)
description WOS:000340742100110
publishDate 2014
dc.date.none.fl_str_mv 2014-08-19
2014-08-19T00:00:00Z
2020-02-05T23:22:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/92249
url http://hdl.handle.net/10362/92249
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
PURE: 1729799
https://doi.org/10.1371/journal.pone.0105588
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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