Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/67556 |
Resumo: | Specific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively. |
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Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb developmentAnimalsChick embryoEctodermFibroblast growth factorsGene expressionGene expression profilingIn situ hybridizationLimb budsMesodermMolecular sequence dataReceptors, Fibroblast growth factorReverse transcriptase polymerase chain reactionSignal TransductionWings, AnimalGene expression regulation, DevelopmentalFgfFgfRslimbchickScience & TechnologySpecific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively.The authors wish to thank Anja Hagemann for critical reading of themanuscript and Ramiro Magno, , Fernanda Bajanca and Tatiana Resendefor their help with the figures. We thank Dr. Guojun Sheng for the gift ofthe FgfR1-4 probes. We also acknowledge Tatiana Queirós for contribut-ing to the initiation of this project. CJS is supported by Fundação para aCiência e Tecnologia (grant n SFRH/BD/33176/2007) and RPA is fundedby a Ciência2007 Program Contract (Portuguese Government). Thiswork was supported by the Fundação para a Ciência e Tecnologia,Portugal (project PTDC/SAU-OBD/099758/2008), and by EU/FP6 - Net-work of Excellence - Cells into Organs (www.cellsintoorgans.net) and by“IBB/CBME, LA, FEDER/POCI 2010”.University of the Basque Country (UPV/EHU)Universidade do MinhoSheeba, Caroline J.Andrade, Raquel P.Duprez, DelphinePalmeirim, Isabel20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/67556eng0214-62821696-354710.1387/ijdb.092887cs21302260info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:36:12Zoai:repositorium.sdum.uminho.pt:1822/67556Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:32:13.086226Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
title |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
spellingShingle |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development Sheeba, Caroline J. Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick Science & Technology |
title_short |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
title_full |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
title_fullStr |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
title_full_unstemmed |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
title_sort |
Comprehensive analysis of fibroblast growth factor receptor expression patterns during chick forelimb development |
author |
Sheeba, Caroline J. |
author_facet |
Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
author_role |
author |
author2 |
Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Sheeba, Caroline J. Andrade, Raquel P. Duprez, Delphine Palmeirim, Isabel |
dc.subject.por.fl_str_mv |
Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick Science & Technology |
topic |
Animals Chick embryo Ectoderm Fibroblast growth factors Gene expression Gene expression profiling In situ hybridization Limb buds Mesoderm Molecular sequence data Receptors, Fibroblast growth factor Reverse transcriptase polymerase chain reaction Signal Transduction Wings, Animal Gene expression regulation, Developmental Fgf FgfRs limb chick Science & Technology |
description |
Specific interactions between fibroblast growth factors (Fgf1-22) and their tyrosine kinase receptors (FgfR1-4) activate different signalling pathways that are responsible for the biological processes in which Fgf signalling is implicated during embryonic development. In the chick, several Fgf ligands (Fgf2, 4, 8, 9, 10, 12, 13 and 18) and the four FgfRs (FgfR 1, 2, 3 and 4) have been reported to be expressed in the developing limb. The precise spatial and temporal expression of these transcripts is important to guide the limb bud to develop into a wing/leg. In this paper, we present a detailed and systematic analysis of the expression patterns of FgfR1, 2, 3 and 4 throughout chick wing development, by in situ hybridisation on whole mounts and sections. Moreover, we characterize for the first time the different isoforms of FGFR1-3 by analysing their differential expression in limb ectoderm and mesodermal tissues, using RT-PCR and in situ hybridisation on sections. Finally, isoform-specific sequences for FgfR1IIIb, FgfR1IIIc, FgfR3IIIb and FgfR3IIIc were determined and deposited in GenBank with the following accession numbers: GU053725, GU065444, GU053726, GU065445, respectively. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010 2010-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/67556 |
url |
http://hdl.handle.net/1822/67556 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0214-6282 1696-3547 10.1387/ijdb.092887cs 21302260 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
University of the Basque Country (UPV/EHU) |
publisher.none.fl_str_mv |
University of the Basque Country (UPV/EHU) |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132833717944320 |