Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella

Detalhes bibliográficos
Autor(a) principal: Araújo, Daniela
Data de Publicação: 2022
Outros Autores: Mil-Homens, Dalila, Henriques, Mariana, Silva, Sónia Carina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/75282
Resumo: EFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2-OMethylRNA oligomer for inhibiting Candida albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single-dose and with a double-dose of the anti-EFG1 2OMe oligomer (at 40 and 100 nM). The anti-EFG1 2OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2OMe oligomer toxicity was observed and the treatment with double-dose of 2OMe oligomer empowered the larvae survival over 24 h (by 90-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2OMe oligomer for controlling C. albicans infections.
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spelling Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella2′-OMethyl chemical modificationantisense oligonucleotidescandidiasisGalleria mellonellavirulenceCiências Naturais::Ciências BiológicasScience & TechnologyEFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2-OMethylRNA oligomer for inhibiting Candida albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single-dose and with a double-dose of the anti-EFG1 2OMe oligomer (at 40 and 100 nM). The anti-EFG1 2OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2OMe oligomer toxicity was observed and the treatment with double-dose of 2OMe oligomer empowered the larvae survival over 24 h (by 90-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2OMe oligomer for controlling C. albicans infections.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and BioTecNorte operation ( NORTE-01-0145-FEDER-000004 ) funded by the European Regional Development Fund under the scope of Norte2–20 – Programa Operacional Regional do Norte and Daniela Eira Araújo [ SFRH/BD/121417/2016 ] PhD grant. The authors also acknowledge the project funding by the “ 02/SAICT/2017 – Projetos de Investigação Científica e Desenvolvimento Tecnológico (IC&DT) – POCI-01-0145-FEDER-028893.” Funding received by IBB – Institute for Bioengineering and Biosciences from FCT ( UID/BIO/04565/2020 ) and Programa Operacional Regional de Lisboa 2020 (project no. 007317 ) is also acknowledged. We acknowledge Dr Lucília Goreti Pinto, Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho , for processing and sectioning tissue samples of G. mellonella.info:eu-repo/semantics/publishedVersionCell PressUniversidade do MinhoAraújo, DanielaMil-Homens, DalilaHenriques, MarianaSilva, Sónia Carina2022-03-082022-03-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/75282engAraújo, Daniela; Mil-Homens, Dalila; Henriques, Mariana; Silva, Sónia Carina, Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella. Molecular Therapy-Nucleic Acids, 27, 517-523, 20222162-253110.1016/j.omtn.2021.12.018https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(21)00321-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:46:40Zoai:repositorium.sdum.uminho.pt:1822/75282Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:44:41.073730Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
title Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
spellingShingle Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
Araújo, Daniela
2′-OMethyl chemical modification
antisense oligonucleotides
candidiasis
Galleria mellonella
virulence
Ciências Naturais::Ciências Biológicas
Science & Technology
title_short Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
title_full Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
title_fullStr Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
title_full_unstemmed Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
title_sort Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella
author Araújo, Daniela
author_facet Araújo, Daniela
Mil-Homens, Dalila
Henriques, Mariana
Silva, Sónia Carina
author_role author
author2 Mil-Homens, Dalila
Henriques, Mariana
Silva, Sónia Carina
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Araújo, Daniela
Mil-Homens, Dalila
Henriques, Mariana
Silva, Sónia Carina
dc.subject.por.fl_str_mv 2′-OMethyl chemical modification
antisense oligonucleotides
candidiasis
Galleria mellonella
virulence
Ciências Naturais::Ciências Biológicas
Science & Technology
topic 2′-OMethyl chemical modification
antisense oligonucleotides
candidiasis
Galleria mellonella
virulence
Ciências Naturais::Ciências Biológicas
Science & Technology
description EFG1 is a central transcriptional regulator of filamentation that is an important virulence factor of Candida albicans. This study serves to assess in vivo the applicability of the anti-EFG1 2-OMethylRNA oligomer for inhibiting Candida albicans filamentation and to attenuate candidiasis, using the Galleria mellonella model. For that, larvae infected with a lethal concentration of C. albicans cells were treated with a single-dose and with a double-dose of the anti-EFG1 2OMe oligomer (at 40 and 100 nM). The anti-EFG1 2OMe oligomer toxicity and effect on larvae survival was evaluated. No evidence of anti-EFG1 2OMe oligomer toxicity was observed and the treatment with double-dose of 2OMe oligomer empowered the larvae survival over 24 h (by 90-100%) and prolonged its efficacy until 72 h of infection (by 30%). Undoubtedly, this work validates the in vivo therapeutic potential of anti-EFG1 2OMe oligomer for controlling C. albicans infections.
publishDate 2022
dc.date.none.fl_str_mv 2022-03-08
2022-03-08T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/75282
url http://hdl.handle.net/1822/75282
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Araújo, Daniela; Mil-Homens, Dalila; Henriques, Mariana; Silva, Sónia Carina, Anti-EFG1 2-OMethylRNA oligomer inhibits Candida albicans filamentation and attenuates the candidiasis in Galleria mellonella. Molecular Therapy-Nucleic Acids, 27, 517-523, 2022
2162-2531
10.1016/j.omtn.2021.12.018
https://www.cell.com/molecular-therapy-family/nucleic-acids/fulltext/S2162-2531(21)00321-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cell Press
publisher.none.fl_str_mv Cell Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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