A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination

Detalhes bibliográficos
Autor(a) principal: Almeida, Sérgio F. de
Data de Publicação: 2010
Outros Autores: García-Sacristán, Ana, Custódio, Noélia, Carmo-Fonseca, Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/6360
Resumo: ©The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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spelling A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional terminationEukaryotesRNA, messengerExosomeRNA polymerase II©The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.In eukaryotes, the production of mature messenger RNA that exits the nucleus to be translated into protein in the cytoplasm requires precise and extensive modification of the nascent transcript. Any failure that compromises the integrity of an mRNA may cause its retention in the nucleus and trigger its degradation. Multiple studies indicate that mRNAs with processing defects accumulate in nuclear foci or ‘dots’ located near the site of transcription, but how exactly are defective RNAs recognized and tethered is still unknown. Here, we present evidence suggesting that unprocessed β-globin transcripts render RNA polymerase II (Pol II) incompetent for termination and that this quality control process requires the integrity of the nuclear exosome. Our results show that unprocessed pre-mRNAs remain tethered to the DNA template in association with Pol II, in an Rrp6-dependent manner. This reveals an unprecedented link between nuclear RNA surveillance, the exosome and Pol II transcriptional termination.Fundação para a Ciência e Tecnologia, Portugal (PTDC/SAU-GMG/69739/2006); European Commission (MCRTN Eurythron 005499 and EURASNET, LSHGCT- 2005-518238). Fellowship from Fundação para a Ciência e Tecnologia (SFRH/BPD/34679/2007 to S.F. de A.). Funding for open access charge: Fundação para a Ciência e Tecnologia, Portugal (PTDC/SAU-GMG/69739/2006).Oxford University PressRepositório da Universidade de LisboaAlmeida, Sérgio F. deGarcía-Sacristán, AnaCustódio, NoéliaCarmo-Fonseca, Maria2012-05-22T15:34:57Z20102010-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/6360engNucleic Acids Research, 2010, Vol. 38, No. 22, 8015–80260305-1048info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T15:48:50Zoai:repositorio.ul.pt:10451/6360Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:31:31.123074Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
title A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
spellingShingle A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
Almeida, Sérgio F. de
Eukaryotes
RNA, messenger
Exosome
RNA polymerase II
title_short A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
title_full A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
title_fullStr A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
title_full_unstemmed A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
title_sort A link between nuclear RNA surveillance, the human exosome and RNA polymerase II transcriptional termination
author Almeida, Sérgio F. de
author_facet Almeida, Sérgio F. de
García-Sacristán, Ana
Custódio, Noélia
Carmo-Fonseca, Maria
author_role author
author2 García-Sacristán, Ana
Custódio, Noélia
Carmo-Fonseca, Maria
author2_role author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Almeida, Sérgio F. de
García-Sacristán, Ana
Custódio, Noélia
Carmo-Fonseca, Maria
dc.subject.por.fl_str_mv Eukaryotes
RNA, messenger
Exosome
RNA polymerase II
topic Eukaryotes
RNA, messenger
Exosome
RNA polymerase II
description ©The Author(s) 2010. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/ by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
publishDate 2010
dc.date.none.fl_str_mv 2010
2010-01-01T00:00:00Z
2012-05-22T15:34:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/6360
url http://hdl.handle.net/10451/6360
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Nucleic Acids Research, 2010, Vol. 38, No. 22, 8015–8026
0305-1048
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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