Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/120026 |
Resumo: | NORTE-01-0145-FEDER-000032 PTDC/BIA-BMA/29754/2017 PD/BD/127994/2016 SFRH/BD/131527/2017 |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic IberiaAtlantic iberiaCytochrome c oxidase subunit IDNA barcodingMacroinvertebratesReference libraryEcology, Evolution, Behavior and SystematicsAquatic ScienceEcologyAnimal Science and ZoologySDG 14 - Life Below WaterNORTE-01-0145-FEDER-000032 PTDC/BIA-BMA/29754/2017 PD/BD/127994/2016 SFRH/BD/131527/2017DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy of species-level assignment, and consequent taxonomic coverage, relies on comprehensive DNA barcode reference libraries, which, due to incompleteness, are currently a recognized limitation for metabarcoding applications. In this study, we assembled a comprehensive reference library of DNA barcodes for Atlantic Iberia marine macroinvertebrate species, assessed gaps in species coverage and examined data ambiguities. Initially, an Iberian species checklist for the three dominant groups of marine macroinvertebrates was compiled, comprising 2827 species (926 Annelida, 638 Crustacea and 1263 Mollusca). A total of 18162 DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) matching the species checklist were compiled in a BOLD dataset, where taxonomic discordances were evaluated and cases of deep intraspecific divergence flagged. Gap-analysis showed that 63% of the Iberian macroinvertebrate species still lack a DNA barcode. Coverage gaps varied considerably across taxonomic groups with Mollusca displaying the highest sequence representation in the dataset (427 species, 49% of the total number of sequences), and Crustacea the highest species coverage with 338 species barcoded (53% of the checklist). In contrast, Polychaeta displayed the lower levels of completion (288 species, 16% of the total number of sequences). In total, 1545 Barcode Index Numbers (BINs) were assigned to 1053 barcoded species, of which 66% were taxonomically concordant, 26% displayed multiple BINs and 8% were discordant. Overall, results show that there is still a large portion of marine invertebrate taxa in this region of Europe pending barcode coverage, even considering only the dominant groups. However, the most notable finding was the relevant proportion of species flagged for significant intraspecific divergence and possible hidden diversity. The annotated reference library and gap-analysis here provided can therefore contribute to prioritize marine macroinvertebrate taxa for future research efforts and barcode coverage.MARE - Centro de Ciências do Mar e do AmbienteRUNLeite, Bárbara R.Vieira, Pedro E.Teixeira, Marcos A. L.Lobo-Arteaga, JorgeHollatz, ClaudiaBorges, Luisa M. S.Duarte, SofiaTroncoso, J. S.Costa, Filipa O.2021-06-25T22:18:34Z2020-042020-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120026eng2352-4855PURE: 32184371https://doi.org/10.1016/j.rsma.2020.101307info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:02:40Zoai:run.unl.pt:10362/120026Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:16.475678Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| title |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| spellingShingle |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia Leite, Bárbara R. Atlantic iberia Cytochrome c oxidase subunit I DNA barcoding Macroinvertebrates Reference library Ecology, Evolution, Behavior and Systematics Aquatic Science Ecology Animal Science and Zoology SDG 14 - Life Below Water |
| title_short |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| title_full |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| title_fullStr |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| title_full_unstemmed |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| title_sort |
Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia |
| author |
Leite, Bárbara R. |
| author_facet |
Leite, Bárbara R. Vieira, Pedro E. Teixeira, Marcos A. L. Lobo-Arteaga, Jorge Hollatz, Claudia Borges, Luisa M. S. Duarte, Sofia Troncoso, J. S. Costa, Filipa O. |
| author_role |
author |
| author2 |
Vieira, Pedro E. Teixeira, Marcos A. L. Lobo-Arteaga, Jorge Hollatz, Claudia Borges, Luisa M. S. Duarte, Sofia Troncoso, J. S. Costa, Filipa O. |
| author2_role |
author author author author author author author author |
| dc.contributor.none.fl_str_mv |
MARE - Centro de Ciências do Mar e do Ambiente RUN |
| dc.contributor.author.fl_str_mv |
Leite, Bárbara R. Vieira, Pedro E. Teixeira, Marcos A. L. Lobo-Arteaga, Jorge Hollatz, Claudia Borges, Luisa M. S. Duarte, Sofia Troncoso, J. S. Costa, Filipa O. |
| dc.subject.por.fl_str_mv |
Atlantic iberia Cytochrome c oxidase subunit I DNA barcoding Macroinvertebrates Reference library Ecology, Evolution, Behavior and Systematics Aquatic Science Ecology Animal Science and Zoology SDG 14 - Life Below Water |
| topic |
Atlantic iberia Cytochrome c oxidase subunit I DNA barcoding Macroinvertebrates Reference library Ecology, Evolution, Behavior and Systematics Aquatic Science Ecology Animal Science and Zoology SDG 14 - Life Below Water |
| description |
NORTE-01-0145-FEDER-000032 PTDC/BIA-BMA/29754/2017 PD/BD/127994/2016 SFRH/BD/131527/2017 |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-04 2020-04-01T00:00:00Z 2021-06-25T22:18:34Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/120026 |
| url |
http://hdl.handle.net/10362/120026 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2352-4855 PURE: 32184371 https://doi.org/10.1016/j.rsma.2020.101307 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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