Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia

Detalhes bibliográficos
Autor(a) principal: Leite, Bárbara R.
Data de Publicação: 2020
Outros Autores: Vieira, Pedro E., Teixeira, Marcos A. L., Lobo-Arteaga, Jorge, Hollatz, Claudia, Borges, Luisa M. S., Duarte, Sofia, Troncoso, J. S., Costa, Filipa O.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/120026
Resumo: NORTE-01-0145-FEDER-000032 PTDC/BIA-BMA/29754/2017 PD/BD/127994/2016 SFRH/BD/131527/2017
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spelling Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic IberiaAtlantic iberiaCytochrome c oxidase subunit IDNA barcodingMacroinvertebratesReference libraryEcology, Evolution, Behavior and SystematicsAquatic ScienceEcologyAnimal Science and ZoologySDG 14 - Life Below WaterNORTE-01-0145-FEDER-000032 PTDC/BIA-BMA/29754/2017 PD/BD/127994/2016 SFRH/BD/131527/2017DNA metabarcoding provides a rapid and effective identification tool of macroinvertebrate species. The accuracy of species-level assignment, and consequent taxonomic coverage, relies on comprehensive DNA barcode reference libraries, which, due to incompleteness, are currently a recognized limitation for metabarcoding applications. In this study, we assembled a comprehensive reference library of DNA barcodes for Atlantic Iberia marine macroinvertebrate species, assessed gaps in species coverage and examined data ambiguities. Initially, an Iberian species checklist for the three dominant groups of marine macroinvertebrates was compiled, comprising 2827 species (926 Annelida, 638 Crustacea and 1263 Mollusca). A total of 18162 DNA sequences of the cytochrome c oxidase subunit I barcode region (COI-5P) matching the species checklist were compiled in a BOLD dataset, where taxonomic discordances were evaluated and cases of deep intraspecific divergence flagged. Gap-analysis showed that 63% of the Iberian macroinvertebrate species still lack a DNA barcode. Coverage gaps varied considerably across taxonomic groups with Mollusca displaying the highest sequence representation in the dataset (427 species, 49% of the total number of sequences), and Crustacea the highest species coverage with 338 species barcoded (53% of the checklist). In contrast, Polychaeta displayed the lower levels of completion (288 species, 16% of the total number of sequences). In total, 1545 Barcode Index Numbers (BINs) were assigned to 1053 barcoded species, of which 66% were taxonomically concordant, 26% displayed multiple BINs and 8% were discordant. Overall, results show that there is still a large portion of marine invertebrate taxa in this region of Europe pending barcode coverage, even considering only the dominant groups. However, the most notable finding was the relevant proportion of species flagged for significant intraspecific divergence and possible hidden diversity. The annotated reference library and gap-analysis here provided can therefore contribute to prioritize marine macroinvertebrate taxa for future research efforts and barcode coverage.MARE - Centro de Ciências do Mar e do AmbienteRUNLeite, Bárbara R.Vieira, Pedro E.Teixeira, Marcos A. L.Lobo-Arteaga, JorgeHollatz, ClaudiaBorges, Luisa M. S.Duarte, SofiaTroncoso, J. S.Costa, Filipa O.2021-06-25T22:18:34Z2020-042020-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120026eng2352-4855PURE: 32184371https://doi.org/10.1016/j.rsma.2020.101307info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:02:40Zoai:run.unl.pt:10362/120026Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:16.475678Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
title Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
spellingShingle Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
Leite, Bárbara R.
Atlantic iberia
Cytochrome c oxidase subunit I
DNA barcoding
Macroinvertebrates
Reference library
Ecology, Evolution, Behavior and Systematics
Aquatic Science
Ecology
Animal Science and Zoology
SDG 14 - Life Below Water
title_short Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
title_full Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
title_fullStr Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
title_full_unstemmed Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
title_sort Gap-analysis and annotated reference library for supporting macroinvertebrate metabarcoding in Atlantic Iberia
author Leite, Bárbara R.
author_facet Leite, Bárbara R.
Vieira, Pedro E.
Teixeira, Marcos A. L.
Lobo-Arteaga, Jorge
Hollatz, Claudia
Borges, Luisa M. S.
Duarte, Sofia
Troncoso, J. S.
Costa, Filipa O.
author_role author
author2 Vieira, Pedro E.
Teixeira, Marcos A. L.
Lobo-Arteaga, Jorge
Hollatz, Claudia
Borges, Luisa M. S.
Duarte, Sofia
Troncoso, J. S.
Costa, Filipa O.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv MARE - Centro de Ciências do Mar e do Ambiente
RUN
dc.contributor.author.fl_str_mv Leite, Bárbara R.
Vieira, Pedro E.
Teixeira, Marcos A. L.
Lobo-Arteaga, Jorge
Hollatz, Claudia
Borges, Luisa M. S.
Duarte, Sofia
Troncoso, J. S.
Costa, Filipa O.
dc.subject.por.fl_str_mv Atlantic iberia
Cytochrome c oxidase subunit I
DNA barcoding
Macroinvertebrates
Reference library
Ecology, Evolution, Behavior and Systematics
Aquatic Science
Ecology
Animal Science and Zoology
SDG 14 - Life Below Water
topic Atlantic iberia
Cytochrome c oxidase subunit I
DNA barcoding
Macroinvertebrates
Reference library
Ecology, Evolution, Behavior and Systematics
Aquatic Science
Ecology
Animal Science and Zoology
SDG 14 - Life Below Water
description NORTE-01-0145-FEDER-000032 PTDC/BIA-BMA/29754/2017 PD/BD/127994/2016 SFRH/BD/131527/2017
publishDate 2020
dc.date.none.fl_str_mv 2020-04
2020-04-01T00:00:00Z
2021-06-25T22:18:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/120026
url http://hdl.handle.net/10362/120026
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 2352-4855
PURE: 32184371
https://doi.org/10.1016/j.rsma.2020.101307
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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