Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens

Detalhes bibliográficos
Autor(a) principal: Alves, Mónica N.
Data de Publicação: 2016
Outros Autores: Fernandes, Ana P., Salgueiro, Carlos A., Paquete, C.M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://doi.org/10.1016/j.bbabio.2015.09.010
Resumo: The authors thank Ricardo O. Louro for his helpful discussions. This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) Portugal [Grants PTDC/QUI-BIQ/117440/2010, UID/Multi/04378/2013; APF and CMP were supported by FCT grants SFRH/BD/86439/2012 and SFRH/BPD/96952/2013, respectively]. The NMR spectrometers are part of The National NMR Facility, supported by FCT (RECI/BBB-BQB/0230/2012).
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spelling Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducensElectron transferExtracellular respirationGeobacterMultiheme cytochromesNanowiresBiophysicsBiochemistryCell BiologyThe authors thank Ricardo O. Louro for his helpful discussions. This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) Portugal [Grants PTDC/QUI-BIQ/117440/2010, UID/Multi/04378/2013; APF and CMP were supported by FCT grants SFRH/BD/86439/2012 and SFRH/BPD/96952/2013, respectively]. The NMR spectrometers are part of The National NMR Facility, supported by FCT (RECI/BBB-BQB/0230/2012).The extracellular electron transfer metabolism of Geobacter sulfurreducens is sustained by several multiheme c-type cytochromes. One of these is the dodecaheme cytochrome GSU1996 that belongs to a new sub-class of c-type cytochromes. GSU1996 is composed by four similar triheme domains (A-D). The C-terminal half of the molecule encompasses the domains C and D, which are connected by a small linker and the N-terminal half of the protein contains two domains (A and B) that form one structural unit. It was proposed that this proteinworks as an electrically conductive device in G. sulfurreducens, transferring electrons within the periplasm or to outer-membrane cytochromes. In this work, a novel strategy was applied to characterize in detail the thermodynamic and kinetic properties of the hexaheme fragment CD of GSU1996. This characterization revealed the electron transfer process of GSU1996 for the first time, showing that a heme at the edge of the C-terminal of the protein is thermodynamic and kinetically competent to receive electrons from physiological redox partners. This information contributes towards understanding how this new sub-class of cytochromes functions as nanowires, and also increases the current knowledge of the extracellular electron transfer mechanisms in G. sulfurreducens.DQ - Departamento de QuímicaUCIBIO - Applied Molecular Biosciences UnitMolecular, Structural and Cellular Microbiology (MOSTMICRO)Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNAlves, Mónica N.Fernandes, Ana P.Salgueiro, Carlos A.Paquete, C.M.2018-05-03T22:04:40Z2016-01-012016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article7application/pdfhttps://doi.org/10.1016/j.bbabio.2015.09.010eng0005-2728PURE: 1581824http://www.scopus.com/inward/record.url?scp=84944929864&partnerID=8YFLogxKhttps://doi.org/10.1016/j.bbabio.2015.09.010info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:19:31Zoai:run.unl.pt:10362/35897Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:30:22.861819Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
title Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
spellingShingle Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
Alves, Mónica N.
Electron transfer
Extracellular respiration
Geobacter
Multiheme cytochromes
Nanowires
Biophysics
Biochemistry
Cell Biology
title_short Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
title_full Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
title_fullStr Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
title_full_unstemmed Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
title_sort Unraveling the electron transfer processes of a nanowire protein from Geobacter sulfurreducens
author Alves, Mónica N.
author_facet Alves, Mónica N.
Fernandes, Ana P.
Salgueiro, Carlos A.
Paquete, C.M.
author_role author
author2 Fernandes, Ana P.
Salgueiro, Carlos A.
Paquete, C.M.
author2_role author
author
author
dc.contributor.none.fl_str_mv DQ - Departamento de Química
UCIBIO - Applied Molecular Biosciences Unit
Molecular, Structural and Cellular Microbiology (MOSTMICRO)
Instituto de Tecnologia Química e Biológica António Xavier (ITQB)
RUN
dc.contributor.author.fl_str_mv Alves, Mónica N.
Fernandes, Ana P.
Salgueiro, Carlos A.
Paquete, C.M.
dc.subject.por.fl_str_mv Electron transfer
Extracellular respiration
Geobacter
Multiheme cytochromes
Nanowires
Biophysics
Biochemistry
Cell Biology
topic Electron transfer
Extracellular respiration
Geobacter
Multiheme cytochromes
Nanowires
Biophysics
Biochemistry
Cell Biology
description The authors thank Ricardo O. Louro for his helpful discussions. This work was supported by Fundacao para a Ciencia e Tecnologia (FCT) Portugal [Grants PTDC/QUI-BIQ/117440/2010, UID/Multi/04378/2013; APF and CMP were supported by FCT grants SFRH/BD/86439/2012 and SFRH/BPD/96952/2013, respectively]. The NMR spectrometers are part of The National NMR Facility, supported by FCT (RECI/BBB-BQB/0230/2012).
publishDate 2016
dc.date.none.fl_str_mv 2016-01-01
2016-01-01T00:00:00Z
2018-05-03T22:04:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://doi.org/10.1016/j.bbabio.2015.09.010
url https://doi.org/10.1016/j.bbabio.2015.09.010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0005-2728
PURE: 1581824
http://www.scopus.com/inward/record.url?scp=84944929864&partnerID=8YFLogxK
https://doi.org/10.1016/j.bbabio.2015.09.010
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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