African trypanosomiasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/125097 |
Resumo: | Funding Information: Funding: This study was supported by FCT—Foundation for Science and Technology, I.P., through research grant PTDC/CVT-CVT/28908/2017 and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020) and Global Health and Tropical Medicine (GHTM, UID/04413/2020). Funding Information: This study was supported by FCT?Foundation for Science and Technology, I.P., through research grant PTDC/CVT-CVT/28908/2017 and by national funds within the scope of Centro de Investiga??o Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020) and Global Health and Tropical Medicine (GHTM, UID/04413/2020). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
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African trypanosomiasisExtracellular vesicles shed by trypanosoma brucei brucei manipulate host mononuclear cellsAfrican trypanosomiasisExosomesMacrophagesRegulatory T cellsT lymphocytesTrypanosoma brucei bruceiInsect ScienceGeneral Immunology and MicrobiologyImmunology and Microbiology (miscellaneous)MicrobiologyParasitologyEpidemiologyInfectious DiseasesEcologyBiochemistry, Genetics and Molecular Biology (miscellaneous)Cell BiologyGeneral Biochemistry,Genetics and Molecular BiologyMolecular BiologyGeneral Veterinaryveterinary (miscalleneous)SDG 1 - No PovertySDG 3 - Good Health and Well-beingFunding Information: Funding: This study was supported by FCT—Foundation for Science and Technology, I.P., through research grant PTDC/CVT-CVT/28908/2017 and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020) and Global Health and Tropical Medicine (GHTM, UID/04413/2020). Funding Information: This study was supported by FCT?Foundation for Science and Technology, I.P., through research grant PTDC/CVT-CVT/28908/2017 and by national funds within the scope of Centro de Investiga??o Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020) and Global Health and Tropical Medicine (GHTM, UID/04413/2020). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.African trypanosomiasis or sleeping sickness is a zoonotic disease caused by Trypanosoma brucei, a protozoan parasite transmitted by Glossina spp. (tsetse fly). Parasite introduction into mammal hosts triggers a succession of events, involving both innate and adaptive immunity. Macrophages (MΦ) have a key role in innate defence since they are antigen-presenting cells and have a micro-bicidal function essential for trypanosome clearance. Adaptive immune defence is carried out by lymphocytes, especially by T cells that promote an integrated immune response. Like mammal cells, T. b. brucei parasites release extracellular vesicles (TbEVs), which carry macromolecules that can be transferred to host cells, transmitting biological information able to manipulate cell immune response. However, the exact role of TbEVs in host immune response remains poorly understood. Thus, the current study examined the effect elicited by TbEVs on MΦ and T lymphocytes. A combined approach of microscopy, nanoparticle tracking analysis, multiparametric flow cytometry, colourimetric assays and detailed statistical analyses were used to evaluate the influence of TbEVs in mouse mononuclear cells. It was shown that TbEVs can establish direct communication with cells of innate and adaptative immunity. TbEVs induce the differentiation of both M1-and M2-MΦ and elicit the expansion of MHCI+, MHCII+ and MHCI+ MHCII+ MΦ subpopulations. In T lymphocytes, TbEVs drive the overexpression of cell-surface CD3 and the nuclear factor FoxP3, which lead to the differentiation of regulatory CD4+ and CD8+ T cells. Moreover, this study indicates that T. b. brucei and TbEVs seem to display opposite but complementary effects in the host, establishing a balance between parasite growth and controlled immune response, at least during the early phase of infection.Instituto de Higiene e Medicina Tropical (IHMT)Global Health and Tropical Medicine (GHTM)Vector borne diseases and pathogens (VBD)RUNDias-Guerreiro, TatianaPalma-Marques, JoanaMourata-Gonçalves, PatríciaAlexandre-Pires, GraçaValério-Bolas, AnaGabriel, ÁureaNunes, TelmoAntunes, WilsonFonseca, Isabel Pereira daSousa-Silva, MarceloSantos-Gomes, Gabriela2021-09-24T02:25:07Z2021-08-202021-08-20T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17application/pdfhttp://hdl.handle.net/10362/125097eng2227-9059PURE: 33792388https://doi.org/10.3390/biomedicines9081056info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:06:18Zoai:run.unl.pt:10362/125097Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:45:39.884485Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
African trypanosomiasis Extracellular vesicles shed by trypanosoma brucei brucei manipulate host mononuclear cells |
| title |
African trypanosomiasis |
| spellingShingle |
African trypanosomiasis Dias-Guerreiro, Tatiana African trypanosomiasis Exosomes Macrophages Regulatory T cells T lymphocytes Trypanosoma brucei brucei Insect Science General Immunology and Microbiology Immunology and Microbiology (miscellaneous) Microbiology Parasitology Epidemiology Infectious Diseases Ecology Biochemistry, Genetics and Molecular Biology (miscellaneous) Cell Biology General Biochemistry,Genetics and Molecular Biology Molecular Biology General Veterinary veterinary (miscalleneous) SDG 1 - No Poverty SDG 3 - Good Health and Well-being |
| title_short |
African trypanosomiasis |
| title_full |
African trypanosomiasis |
| title_fullStr |
African trypanosomiasis |
| title_full_unstemmed |
African trypanosomiasis |
| title_sort |
African trypanosomiasis |
| author |
Dias-Guerreiro, Tatiana |
| author_facet |
Dias-Guerreiro, Tatiana Palma-Marques, Joana Mourata-Gonçalves, Patrícia Alexandre-Pires, Graça Valério-Bolas, Ana Gabriel, Áurea Nunes, Telmo Antunes, Wilson Fonseca, Isabel Pereira da Sousa-Silva, Marcelo Santos-Gomes, Gabriela |
| author_role |
author |
| author2 |
Palma-Marques, Joana Mourata-Gonçalves, Patrícia Alexandre-Pires, Graça Valério-Bolas, Ana Gabriel, Áurea Nunes, Telmo Antunes, Wilson Fonseca, Isabel Pereira da Sousa-Silva, Marcelo Santos-Gomes, Gabriela |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
Instituto de Higiene e Medicina Tropical (IHMT) Global Health and Tropical Medicine (GHTM) Vector borne diseases and pathogens (VBD) RUN |
| dc.contributor.author.fl_str_mv |
Dias-Guerreiro, Tatiana Palma-Marques, Joana Mourata-Gonçalves, Patrícia Alexandre-Pires, Graça Valério-Bolas, Ana Gabriel, Áurea Nunes, Telmo Antunes, Wilson Fonseca, Isabel Pereira da Sousa-Silva, Marcelo Santos-Gomes, Gabriela |
| dc.subject.por.fl_str_mv |
African trypanosomiasis Exosomes Macrophages Regulatory T cells T lymphocytes Trypanosoma brucei brucei Insect Science General Immunology and Microbiology Immunology and Microbiology (miscellaneous) Microbiology Parasitology Epidemiology Infectious Diseases Ecology Biochemistry, Genetics and Molecular Biology (miscellaneous) Cell Biology General Biochemistry,Genetics and Molecular Biology Molecular Biology General Veterinary veterinary (miscalleneous) SDG 1 - No Poverty SDG 3 - Good Health and Well-being |
| topic |
African trypanosomiasis Exosomes Macrophages Regulatory T cells T lymphocytes Trypanosoma brucei brucei Insect Science General Immunology and Microbiology Immunology and Microbiology (miscellaneous) Microbiology Parasitology Epidemiology Infectious Diseases Ecology Biochemistry, Genetics and Molecular Biology (miscellaneous) Cell Biology General Biochemistry,Genetics and Molecular Biology Molecular Biology General Veterinary veterinary (miscalleneous) SDG 1 - No Poverty SDG 3 - Good Health and Well-being |
| description |
Funding Information: Funding: This study was supported by FCT—Foundation for Science and Technology, I.P., through research grant PTDC/CVT-CVT/28908/2017 and by national funds within the scope of Centro de Investigação Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020) and Global Health and Tropical Medicine (GHTM, UID/04413/2020). Funding Information: This study was supported by FCT?Foundation for Science and Technology, I.P., through research grant PTDC/CVT-CVT/28908/2017 and by national funds within the scope of Centro de Investiga??o Interdisciplinar em Sanidade Animal (CIISA, UIDB/00276/2020) and Global Health and Tropical Medicine (GHTM, UID/04413/2020). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
| publishDate |
2021 |
| dc.date.none.fl_str_mv |
2021-09-24T02:25:07Z 2021-08-20 2021-08-20T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/125097 |
| url |
http://hdl.handle.net/10362/125097 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2227-9059 PURE: 33792388 https://doi.org/10.3390/biomedicines9081056 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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17 application/pdf |
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RCAAP |
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