Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies

Detalhes bibliográficos
Autor(a) principal: Santos, Jorge Miguel Martins
Data de Publicação: 2013
Tipo de documento: Dissertação
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10362/12214
Resumo: Dissertation to obtain the degree of Master in Chemical and Biochemical Engineering
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spelling Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studiesEnhanced biological phosphorus removal (EBPR)Candidatus Accumulibacter phosphatisTetrasphaera-related organismsMetabolic modelActivated sludge model (ASM)GPS-XDissertation to obtain the degree of Master in Chemical and Biochemical EngineeringThe enhanced biological phosphorus removal (EBPR) process in activated sludge systems has become a widely applied wastewater treatment technology to control eutrophication. The success of this process relies on the sludge enrichment with polyphosphate accumulating organisms (PAOs), while one of the main causes for its failure is due to microbial competition between PAOs and another group of organisms known as the glycogen accumulating organisms (GAOs). The microbial ecology and ecophysiology of these two groups have been investigated through metabolic modelling and experimental studies in order to provide a better understanding of EBPR systems. This thesis focuses on researching the P removal efficiency and metabolic behaviour of an enriched culture containing two PAOs: Tetrasphaera-related organisms and Accumulibacter, which were acclimatized with casamino acids as sole carbon source in a sequencing batch reactor (SBR). Both organisms were identified through fluorescence in situ hybridization (FISH), and this culture demonstrated anaerobic P release, glycogen hydrolysis, a very low poly--hydroxyalkanoates (PHA) synthesis and high casamino acids uptake; followed by aerobic P uptake, glycogen formation and a very low PHA oxidation. Different carbon sources (glucose, acetate, propionate, glutamate, aspartate, glycine and casamino acids) were studied through batch tests inoculated with sludge from the main SBR. Through experimental data, it was suggested that Accumulibacter were responsible for the uptake of volatile fatty acids (VFAs), and Tetrasphaera-related organisms were likely responsible for both glucose and amino acids uptake. This thesis also focuses on the development of a model that combines a PAO-GAO metabolic model with activated sludge model no. 2d (ASM2d) in collaboration with Hydromantis Environmental Software Solutions, Inc.. The combined model was implemented in the GPS-X software and will provide a new and advanced platform for wastewater treatment modelling, which will be available to practitioners.Faculdade de Ciências e TecnologiaOehmen, AdrianRUNSantos, Jorge Miguel Martins2014-06-23T11:03:43Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfhttp://hdl.handle.net/10362/12214enginfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T03:47:05Zoai:run.unl.pt:10362/12214Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:20:48.608366Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
title Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
spellingShingle Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
Santos, Jorge Miguel Martins
Enhanced biological phosphorus removal (EBPR)
Candidatus Accumulibacter phosphatis
Tetrasphaera-related organisms
Metabolic model
Activated sludge model (ASM)
GPS-X
title_short Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
title_full Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
title_fullStr Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
title_full_unstemmed Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
title_sort Understanding the microbial ecology and ecophysiology of enhanced biological phosphorus removal processes through metabolic modelling and experimental studies
author Santos, Jorge Miguel Martins
author_facet Santos, Jorge Miguel Martins
author_role author
dc.contributor.none.fl_str_mv Oehmen, Adrian
RUN
dc.contributor.author.fl_str_mv Santos, Jorge Miguel Martins
dc.subject.por.fl_str_mv Enhanced biological phosphorus removal (EBPR)
Candidatus Accumulibacter phosphatis
Tetrasphaera-related organisms
Metabolic model
Activated sludge model (ASM)
GPS-X
topic Enhanced biological phosphorus removal (EBPR)
Candidatus Accumulibacter phosphatis
Tetrasphaera-related organisms
Metabolic model
Activated sludge model (ASM)
GPS-X
description Dissertation to obtain the degree of Master in Chemical and Biochemical Engineering
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2014-06-23T11:03:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
format masterThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10362/12214
url http://hdl.handle.net/10362/12214
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
publisher.none.fl_str_mv Faculdade de Ciências e Tecnologia
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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