Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant

Detalhes bibliográficos
Autor(a) principal: Alonso, Isabel
Data de Publicação: 2008
Outros Autores: Marques, Joana M., Sousa, Nuno, Sequeiros, Jorge, Olsson, I. Anna S., Silveira, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/61412
Resumo: Supplementary data associated with this article can be found, in the online version, at doi:10.1016/j.neurobiolaging.
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spelling Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutantAnimalsCalcium Channels, N-TypeCognition DisordersMiceMice, Inbred C57BLMovement DisordersMutationAgingLearningrotarod testwater maze testnatural mutantcalcium currentsmemoryScience & TechnologySupplementary data associated with this article can be found, in the online version, at doi:10.1016/j.neurobiolaging.The leaner mutation in mice affects the Ca(v)2.1 voltage-gated calcium channel alpha(1A)-subunit gene (Cacna1a), causing a reduction in calcium currents predominantly in Purkinje cells. This reduction in calcium currents causes severe progressive cerebellar ataxia, beginning around postnatal day 10, in homozygous leaner mice (tg(la)/tg(la)), while their heterozygous littermates (tg(la)/+) present no obvious behavioral deficits. In humans, heterozygous mutations in the Cacna1a orthologous gene produce a broad range of neurological manifestations. To evaluate the phenotypic status of the tg(la)/+ animals, we assessed motor performance and cognition, at different ages, in these mutant mice. We were able to observe age-dependent impairment in motor and cognitive tasks; balance and motor learning deficits were found in demanding tasks on the rotarod and on the hanging wire test, while spatial learning and memory impairment was observed in the Morris water maze. Progressive dysfunction in escape reflexes, indicative of neurological impairment, was also present in tg(la)/+ animals. Although not presenting major motor alterations, tg(la)/+ mice show age-dependent motor and cognitive deficits.We would like to thank Carolina Lemos for her help with statistical analysis and Victor Mendes for image technical assistance. This work was supported by research grants POCTI/MGI/34517/00, POCTI/NSE/45352/2002 and POCI/SAU-MMO/56387/2004, FCT (Fundação para a Ciência e Tecnologia) and co-funded by FEDER. I.A. is recipient of a scholarship from FCT, Portugal.ElsevierUniversidade do MinhoAlonso, IsabelMarques, Joana M.Sousa, NunoSequeiros, JorgeOlsson, I. Anna S.Silveira, Isabel2008-112008-11-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/61412eng0197-458010.1016/j.neurobiolaging.2007.04.00517513018info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:21:37Zoai:repositorium.sdum.uminho.pt:1822/61412Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:14:55.884610Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
title Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
spellingShingle Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
Alonso, Isabel
Animals
Calcium Channels, N-Type
Cognition Disorders
Mice
Mice, Inbred C57BL
Movement Disorders
Mutation
Aging
Learning
rotarod test
water maze test
natural mutant
calcium currents
memory
Science & Technology
title_short Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
title_full Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
title_fullStr Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
title_full_unstemmed Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
title_sort Motor and cognitive deficits in the heterozygous leaner mouse, a Cav2.1 voltage-gated Ca2+ channel mutant
author Alonso, Isabel
author_facet Alonso, Isabel
Marques, Joana M.
Sousa, Nuno
Sequeiros, Jorge
Olsson, I. Anna S.
Silveira, Isabel
author_role author
author2 Marques, Joana M.
Sousa, Nuno
Sequeiros, Jorge
Olsson, I. Anna S.
Silveira, Isabel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Alonso, Isabel
Marques, Joana M.
Sousa, Nuno
Sequeiros, Jorge
Olsson, I. Anna S.
Silveira, Isabel
dc.subject.por.fl_str_mv Animals
Calcium Channels, N-Type
Cognition Disorders
Mice
Mice, Inbred C57BL
Movement Disorders
Mutation
Aging
Learning
rotarod test
water maze test
natural mutant
calcium currents
memory
Science & Technology
topic Animals
Calcium Channels, N-Type
Cognition Disorders
Mice
Mice, Inbred C57BL
Movement Disorders
Mutation
Aging
Learning
rotarod test
water maze test
natural mutant
calcium currents
memory
Science & Technology
description Supplementary data associated with this article can be found, in the online version, at doi:10.1016/j.neurobiolaging.
publishDate 2008
dc.date.none.fl_str_mv 2008-11
2008-11-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/61412
url http://hdl.handle.net/1822/61412
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0197-4580
10.1016/j.neurobiolaging.2007.04.005
17513018
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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