Temporal oral microbiome changes with brushing in children with cleft lip and palate
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/104619 https://doi.org/10.1016/j.heliyon.2021.e06513 |
Resumo: | This cohort study aimed to characterize the oral microbiome of children with CLP, from two different age groups, and evaluate the effect of supervised or unsupervised toothbrushing on the microbiome of the cleft over time. Swab samples were collected from the cleft area at three different time points (A; no brushing, B; after 15 days and C; after 30 days) and were analyzed using next-generation sequencing to determine the microbial composition and diversity in these time points. Overall, brushing significantly decreased the abundance of the genera Alloprevotella and Leptotrichia in the two age groups examined, and for Alloprevotella this decrease was more evident for children (2-6 years old). In the preteen group (7-12 years old), a significant relative increase of the genus Rothia was observed after brushing. In this study, the systematic brushing over a period of thirty days also resulted in differences at the intra-individual bacterial richness. |
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Temporal oral microbiome changes with brushing in children with cleft lip and palateMicrobiomeChildren and preteenCLPNext-generation sequencingAlloprevotellaLeptotrichiaRothiaThis cohort study aimed to characterize the oral microbiome of children with CLP, from two different age groups, and evaluate the effect of supervised or unsupervised toothbrushing on the microbiome of the cleft over time. Swab samples were collected from the cleft area at three different time points (A; no brushing, B; after 15 days and C; after 30 days) and were analyzed using next-generation sequencing to determine the microbial composition and diversity in these time points. Overall, brushing significantly decreased the abundance of the genera Alloprevotella and Leptotrichia in the two age groups examined, and for Alloprevotella this decrease was more evident for children (2-6 years old). In the preteen group (7-12 years old), a significant relative increase of the genus Rothia was observed after brushing. In this study, the systematic brushing over a period of thirty days also resulted in differences at the intra-individual bacterial richness.Elsevier2021-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/104619http://hdl.handle.net/10316/104619https://doi.org/10.1016/j.heliyon.2021.e06513eng2405-8440Rodrigues, RitaChung, Ana P.Mortensen, Martin S.Fernandes, Maria H.Monteiro, António B.Furfuro, RowneySilva, Cátia C.Manso, Maria C.Sørensen, Søren J.Morais, Paula V.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-01-20T21:51:52Zoai:estudogeral.uc.pt:10316/104619Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:21:18.010622Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
title |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
spellingShingle |
Temporal oral microbiome changes with brushing in children with cleft lip and palate Rodrigues, Rita Microbiome Children and preteen CLP Next-generation sequencing Alloprevotella Leptotrichia Rothia |
title_short |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
title_full |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
title_fullStr |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
title_full_unstemmed |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
title_sort |
Temporal oral microbiome changes with brushing in children with cleft lip and palate |
author |
Rodrigues, Rita |
author_facet |
Rodrigues, Rita Chung, Ana P. Mortensen, Martin S. Fernandes, Maria H. Monteiro, António B. Furfuro, Rowney Silva, Cátia C. Manso, Maria C. Sørensen, Søren J. Morais, Paula V. |
author_role |
author |
author2 |
Chung, Ana P. Mortensen, Martin S. Fernandes, Maria H. Monteiro, António B. Furfuro, Rowney Silva, Cátia C. Manso, Maria C. Sørensen, Søren J. Morais, Paula V. |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Rodrigues, Rita Chung, Ana P. Mortensen, Martin S. Fernandes, Maria H. Monteiro, António B. Furfuro, Rowney Silva, Cátia C. Manso, Maria C. Sørensen, Søren J. Morais, Paula V. |
dc.subject.por.fl_str_mv |
Microbiome Children and preteen CLP Next-generation sequencing Alloprevotella Leptotrichia Rothia |
topic |
Microbiome Children and preteen CLP Next-generation sequencing Alloprevotella Leptotrichia Rothia |
description |
This cohort study aimed to characterize the oral microbiome of children with CLP, from two different age groups, and evaluate the effect of supervised or unsupervised toothbrushing on the microbiome of the cleft over time. Swab samples were collected from the cleft area at three different time points (A; no brushing, B; after 15 days and C; after 30 days) and were analyzed using next-generation sequencing to determine the microbial composition and diversity in these time points. Overall, brushing significantly decreased the abundance of the genera Alloprevotella and Leptotrichia in the two age groups examined, and for Alloprevotella this decrease was more evident for children (2-6 years old). In the preteen group (7-12 years old), a significant relative increase of the genus Rothia was observed after brushing. In this study, the systematic brushing over a period of thirty days also resulted in differences at the intra-individual bacterial richness. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/104619 http://hdl.handle.net/10316/104619 https://doi.org/10.1016/j.heliyon.2021.e06513 |
url |
http://hdl.handle.net/10316/104619 https://doi.org/10.1016/j.heliyon.2021.e06513 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2405-8440 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
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1799134103599054848 |