Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10362/120782 |
Resumo: | Microbial electrochemical technologies (METs) have emerged in recent years as a promising alternative green source of energy, with microbes consuming organic matter to produce energy or valuable byproducts. It is the ability of performing extracellular electron transfer that allows these microbes to exchange electrons with an electrode in these systems. The low levels of current achieved have been the limiting factor for the large-scale application of METs. Shewanella oneidensis MR-1 is one of the most studied electroactive organisms regarding extracellular electron transfer, and it has been shown that biofilm formation is a key factor for current generation. The transcription factor bolA has been identified as a central player in biofilm formation in other organisms, with its overexpression leading to increased biofilm. In this work we explore the effect of this gene in biofilm formation and current production by S. oneidensis MR-1. Our results demonstrate that an increased biofilm formation and consequent current generation was achieved by the overexpression of this gene. This information is crucial to optimize electroactive organisms toward their practical application in METs. |
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Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1bioelectrochemical systemsbiofilmsbolAelectroactive organismsmicrobial electrochemical technologiestranscription factorMicrobiologyMicrobiology (medical)Microbial electrochemical technologies (METs) have emerged in recent years as a promising alternative green source of energy, with microbes consuming organic matter to produce energy or valuable byproducts. It is the ability of performing extracellular electron transfer that allows these microbes to exchange electrons with an electrode in these systems. The low levels of current achieved have been the limiting factor for the large-scale application of METs. Shewanella oneidensis MR-1 is one of the most studied electroactive organisms regarding extracellular electron transfer, and it has been shown that biofilm formation is a key factor for current generation. The transcription factor bolA has been identified as a central player in biofilm formation in other organisms, with its overexpression leading to increased biofilm. In this work we explore the effect of this gene in biofilm formation and current production by S. oneidensis MR-1. Our results demonstrate that an increased biofilm formation and consequent current generation was achieved by the overexpression of this gene. This information is crucial to optimize electroactive organisms toward their practical application in METs.Instituto de Tecnologia Química e Biológica António Xavier (ITQB)RUNda Silva, Ana VieiraEdel, MiriamGescher, JohannesPaquete, Catarina M.2021-07-09T22:17:17Z2020-05-082020-05-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/120782eng1664-302XPURE: 28011055https://doi.org/10.3389/fmicb.2020.00815info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T05:03:14Zoai:run.unl.pt:10362/120782Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:44:26.768467Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
title |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
spellingShingle |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 da Silva, Ana Vieira bioelectrochemical systems biofilms bolA electroactive organisms microbial electrochemical technologies transcription factor Microbiology Microbiology (medical) |
title_short |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
title_full |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
title_fullStr |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
title_full_unstemmed |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
title_sort |
Exploring the Effects of bolA in Biofilm Formation and Current Generation by Shewanella oneidensis MR-1 |
author |
da Silva, Ana Vieira |
author_facet |
da Silva, Ana Vieira Edel, Miriam Gescher, Johannes Paquete, Catarina M. |
author_role |
author |
author2 |
Edel, Miriam Gescher, Johannes Paquete, Catarina M. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Instituto de Tecnologia Química e Biológica António Xavier (ITQB) RUN |
dc.contributor.author.fl_str_mv |
da Silva, Ana Vieira Edel, Miriam Gescher, Johannes Paquete, Catarina M. |
dc.subject.por.fl_str_mv |
bioelectrochemical systems biofilms bolA electroactive organisms microbial electrochemical technologies transcription factor Microbiology Microbiology (medical) |
topic |
bioelectrochemical systems biofilms bolA electroactive organisms microbial electrochemical technologies transcription factor Microbiology Microbiology (medical) |
description |
Microbial electrochemical technologies (METs) have emerged in recent years as a promising alternative green source of energy, with microbes consuming organic matter to produce energy or valuable byproducts. It is the ability of performing extracellular electron transfer that allows these microbes to exchange electrons with an electrode in these systems. The low levels of current achieved have been the limiting factor for the large-scale application of METs. Shewanella oneidensis MR-1 is one of the most studied electroactive organisms regarding extracellular electron transfer, and it has been shown that biofilm formation is a key factor for current generation. The transcription factor bolA has been identified as a central player in biofilm formation in other organisms, with its overexpression leading to increased biofilm. In this work we explore the effect of this gene in biofilm formation and current production by S. oneidensis MR-1. Our results demonstrate that an increased biofilm formation and consequent current generation was achieved by the overexpression of this gene. This information is crucial to optimize electroactive organisms toward their practical application in METs. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-05-08 2020-05-08T00:00:00Z 2021-07-09T22:17:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/120782 |
url |
http://hdl.handle.net/10362/120782 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1664-302X PURE: 28011055 https://doi.org/10.3389/fmicb.2020.00815 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
institution |
RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799138052502716416 |