Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study

Detalhes bibliográficos
Autor(a) principal: Dias, Rita S.
Data de Publicação: 2005
Outros Autores: Innerlohinger, Josef, Glatter, Otto, Miguel, Maria G., Lindman, Björn
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10316/10328
https://doi.org/10.1021/jp0444464
Resumo: The compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy.
id RCAP_a8612360605090843f8117a7f817e1ac
oai_identifier_str oai:estudogeral.uc.pt:10316/10328
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering StudyThe compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy.American Chemical Society2005-05-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10328http://hdl.handle.net/10316/10328https://doi.org/10.1021/jp0444464engThe Journal of Physical Chemistry B. 109:20 (2005) 10458-104631520-6106Dias, Rita S.Innerlohinger, JosefGlatter, OttoMiguel, Maria G.Lindman, Björninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-25T13:13:03Zoai:estudogeral.uc.pt:10316/10328Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:12.582239Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
title Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
spellingShingle Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
Dias, Rita S.
title_short Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
title_full Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
title_fullStr Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
title_full_unstemmed Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
title_sort Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
author Dias, Rita S.
author_facet Dias, Rita S.
Innerlohinger, Josef
Glatter, Otto
Miguel, Maria G.
Lindman, Björn
author_role author
author2 Innerlohinger, Josef
Glatter, Otto
Miguel, Maria G.
Lindman, Björn
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Dias, Rita S.
Innerlohinger, Josef
Glatter, Otto
Miguel, Maria G.
Lindman, Björn
description The compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy.
publishDate 2005
dc.date.none.fl_str_mv 2005-05-26
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10316/10328
http://hdl.handle.net/10316/10328
https://doi.org/10.1021/jp0444464
url http://hdl.handle.net/10316/10328
https://doi.org/10.1021/jp0444464
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv The Journal of Physical Chemistry B. 109:20 (2005) 10458-10463
1520-6106
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1817550620032237568