Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/10328 https://doi.org/10.1021/jp0444464 |
Resumo: | The compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy. |
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Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering StudyThe compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy.American Chemical Society2005-05-26info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/10328http://hdl.handle.net/10316/10328https://doi.org/10.1021/jp0444464engThe Journal of Physical Chemistry B. 109:20 (2005) 10458-104631520-6106Dias, Rita S.Innerlohinger, JosefGlatter, OttoMiguel, Maria G.Lindman, Björninfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-05-25T13:13:03Zoai:estudogeral.uc.pt:10316/10328Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:01:12.582239Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
title |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
spellingShingle |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study Dias, Rita S. |
title_short |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
title_full |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
title_fullStr |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
title_full_unstemmed |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
title_sort |
Coil−Globule Transition of DNA Molecules Induced by Cationic Surfactants: A Dynamic Light Scattering Study |
author |
Dias, Rita S. |
author_facet |
Dias, Rita S. Innerlohinger, Josef Glatter, Otto Miguel, Maria G. Lindman, Björn |
author_role |
author |
author2 |
Innerlohinger, Josef Glatter, Otto Miguel, Maria G. Lindman, Björn |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Dias, Rita S. Innerlohinger, Josef Glatter, Otto Miguel, Maria G. Lindman, Björn |
description |
The compaction and aggregation of DNA induced by cationic surfactants was studied by dynamic light scattering (DLS). Furthermore, the effect on surfactant-compacted DNA of the addition of nonionic amphiphiles and salt was studied. When using sufficiently low amounts of DNA and cetyltrimethylammonium bromide (CTAB), compacted DNA molecules could be monitored by the appearance of a band characterized by lower hydrodynamic radius and by the decrease in the intensity of the peak corresponding to extended DNA molecules. Notably, we observed a region where compacted molecules coexist with extended ones; these two populations were found to be stable with time. For higher concentrations of CTAB, only compacted molecules were observed and the size of the particles increased with time indicating aggregation. The number of globules present in the coexistence region increased linearly with the surfactant concentrations, as given by the area of the band corresponding to this population, which indicates a double-cooperativity of the binding. The DLS experiments were in good agreement with previous fluorescence microscopy studies, with certain advantages over this technique since there is no need to add fluorescence dyes and antioxidants. Furthermore, it allows the study of molecules which are too small to be visualized by fluorescence microscopy. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-05-26 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/10328 http://hdl.handle.net/10316/10328 https://doi.org/10.1021/jp0444464 |
url |
http://hdl.handle.net/10316/10328 https://doi.org/10.1021/jp0444464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
The Journal of Physical Chemistry B. 109:20 (2005) 10458-10463 1520-6106 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
American Chemical Society |
publisher.none.fl_str_mv |
American Chemical Society |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1817550620032237568 |