Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates

Detalhes bibliográficos
Autor(a) principal: Trobajo-Sanmartín, Camino
Data de Publicação: 2018
Outros Autores: Ezpeleta, Guillermo, Pais, Célia, Eraso, Elena, Quindós, Guillermo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/65928
Resumo: Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated.
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spelling Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolatesCandida parapsilosisCandidiasis, InvasiveDNA, FungalGenotyping TechniquesHumansMultiplex Polymerase Chain ReactionMycological Typing TechniquesSensitivity and SpecificityMicrosatellite RepeatsCandida parapsilosis sensu strictoGenotyping techniqueMicrosatellites repeatsMultiplex PCRSpecificityReproducibility of resultsScience & TechnologyAnalysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated.This work was supported by the Consejeria de Educacion, Universidades e Investigacion of the Gobierno Vasco-Eusko Jaurlaritza [GIC 15/78 IT-990-16] and the Universidad del Pais Vasco/Euskal Herriko Unibertsitatea [UFI 11/25]. CTS has been supported by a research grant of the Fundacion Jesus de Gangoiti Barrera.BioMed Central (BMC)Universidade do MinhoTrobajo-Sanmartín, CaminoEzpeleta, GuillermoPais, CéliaEraso, ElenaQuindós, Guillermo2018-09-292018-09-29T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65928engTrobajo-Sanmartín, C., Ezpeleta, G., Pais, C. et al. Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates. BMC Genomics 19, 718 (2018). https://doi.org/10.1186/s12864-018-5065-31471-216410.1186/s12864-018-5065-330268088https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-018-5065-3info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:12:48Zoai:repositorium.sdum.uminho.pt:1822/65928Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:04:48.342657Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
title Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
spellingShingle Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
Trobajo-Sanmartín, Camino
Candida parapsilosis
Candidiasis, Invasive
DNA, Fungal
Genotyping Techniques
Humans
Multiplex Polymerase Chain Reaction
Mycological Typing Techniques
Sensitivity and Specificity
Microsatellite Repeats
Candida parapsilosis sensu stricto
Genotyping technique
Microsatellites repeats
Multiplex PCR
Specificity
Reproducibility of results
Science & Technology
title_short Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
title_full Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
title_fullStr Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
title_full_unstemmed Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
title_sort Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates
author Trobajo-Sanmartín, Camino
author_facet Trobajo-Sanmartín, Camino
Ezpeleta, Guillermo
Pais, Célia
Eraso, Elena
Quindós, Guillermo
author_role author
author2 Ezpeleta, Guillermo
Pais, Célia
Eraso, Elena
Quindós, Guillermo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Trobajo-Sanmartín, Camino
Ezpeleta, Guillermo
Pais, Célia
Eraso, Elena
Quindós, Guillermo
dc.subject.por.fl_str_mv Candida parapsilosis
Candidiasis, Invasive
DNA, Fungal
Genotyping Techniques
Humans
Multiplex Polymerase Chain Reaction
Mycological Typing Techniques
Sensitivity and Specificity
Microsatellite Repeats
Candida parapsilosis sensu stricto
Genotyping technique
Microsatellites repeats
Multiplex PCR
Specificity
Reproducibility of results
Science & Technology
topic Candida parapsilosis
Candidiasis, Invasive
DNA, Fungal
Genotyping Techniques
Humans
Multiplex Polymerase Chain Reaction
Mycological Typing Techniques
Sensitivity and Specificity
Microsatellite Repeats
Candida parapsilosis sensu stricto
Genotyping technique
Microsatellites repeats
Multiplex PCR
Specificity
Reproducibility of results
Science & Technology
description Analysis of polymorphic microsatellite markers (STR) is a helpful genotyping technique to differentiate Candida parapsilosis sensu stricto isolates. The aim of this study is to develop and perform an initial validation of an alternative protocol for the reliable and accurate microsatellite genotyping of C. parapsilosis sensu stricto isolates using high-throughput multiplex PCR. To achieve this, the results obtained using the new protocol were compared to the ones obtained using a previously described reference method. To that end, diagnostic accuracy, informativeness and discrimination parameters were estimated.
publishDate 2018
dc.date.none.fl_str_mv 2018-09-29
2018-09-29T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/65928
url http://hdl.handle.net/1822/65928
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Trobajo-Sanmartín, C., Ezpeleta, G., Pais, C. et al. Design and validation of a multiplex PCR protocol for microsatellite typing of Candida parapsilosis sensu stricto isolates. BMC Genomics 19, 718 (2018). https://doi.org/10.1186/s12864-018-5065-3
1471-2164
10.1186/s12864-018-5065-3
30268088
https://bmcgenomics.biomedcentral.com/track/pdf/10.1186/s12864-018-5065-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central (BMC)
publisher.none.fl_str_mv BioMed Central (BMC)
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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