Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica

Detalhes bibliográficos
Autor(a) principal: Gomes, Nelma
Data de Publicação: 2013
Outros Autores: Braga, Adelaide, Teixeira, J. A., Belo, Isabel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/25523
Resumo: γ-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the γ-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L−1 was found to be the optimal oil concentration. Under these conditions, cells were able to produce lipase but at low activity levels, which might limit ricinoleic acid release and consequently, the γ-decalactone production rate. Thus, the enzymatic hydrolysis of CO by commercial lipases was studied under different operating conditions. Lipozyme TL IM was found to be the most efficient and the optimal hydrolysis conditions were pH 8 and 27 °C. The use of hydrolyzed CO in the aroma production allowed a decrease in the lag phase for γ-decalactone secretion.
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spelling Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolyticaCastor oilEnzymatic hydrolysisγ-DecalactoneLipaseYarrowia lipolyticagamma-Decalactone?-DecalactoneScience & Technologyγ-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the γ-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L−1 was found to be the optimal oil concentration. Under these conditions, cells were able to produce lipase but at low activity levels, which might limit ricinoleic acid release and consequently, the γ-decalactone production rate. Thus, the enzymatic hydrolysis of CO by commercial lipases was studied under different operating conditions. Lipozyme TL IM was found to be the most efficient and the optimal hydrolysis conditions were pH 8 and 27 °C. The use of hydrolyzed CO in the aroma production allowed a decrease in the lag phase for γ-decalactone secretion.The authors acknowledge Fundacao para a Ciencia e Tecnologia (FCT) for the financial support provided (SFRH/BD/28039/2006 and SFRH/BD/63701/2009).SpringerAmerican oil chemists' society PressUniversidade do MinhoGomes, NelmaBraga, AdelaideTeixeira, J. A.Belo, Isabel20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/25523eng0003-021X1558-933110.1007/s11746-013-2231-2info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-11T04:36:20Zoai:repositorium.sdum.uminho.pt:1822/25523Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-11T04:36:20Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
title Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
spellingShingle Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
Gomes, Nelma
Castor oil
Enzymatic hydrolysis
γ-Decalactone
Lipase
Yarrowia lipolytica
gamma-Decalactone
?-Decalactone
Science & Technology
title_short Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
title_full Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
title_fullStr Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
title_full_unstemmed Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
title_sort Impact of lipase-mediated hydrolysis of castor oil on γ-decalactone production by Yarrowia lipolytica
author Gomes, Nelma
author_facet Gomes, Nelma
Braga, Adelaide
Teixeira, J. A.
Belo, Isabel
author_role author
author2 Braga, Adelaide
Teixeira, J. A.
Belo, Isabel
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gomes, Nelma
Braga, Adelaide
Teixeira, J. A.
Belo, Isabel
dc.subject.por.fl_str_mv Castor oil
Enzymatic hydrolysis
γ-Decalactone
Lipase
Yarrowia lipolytica
gamma-Decalactone
?-Decalactone
Science & Technology
topic Castor oil
Enzymatic hydrolysis
γ-Decalactone
Lipase
Yarrowia lipolytica
gamma-Decalactone
?-Decalactone
Science & Technology
description γ-Decalactone is an industrially interesting peach-like aroma compound that can be produced biotechnologically through the biotransformation of ricinoleic acid. Castor oil (CO) is the raw material most used as the ricinoleic acid source. The effect of different CO concentrations on the γ-decalactone production by Yarrowia lipolytica was investigated in batch processing, and 30 g L−1 was found to be the optimal oil concentration. Under these conditions, cells were able to produce lipase but at low activity levels, which might limit ricinoleic acid release and consequently, the γ-decalactone production rate. Thus, the enzymatic hydrolysis of CO by commercial lipases was studied under different operating conditions. Lipozyme TL IM was found to be the most efficient and the optimal hydrolysis conditions were pH 8 and 27 °C. The use of hydrolyzed CO in the aroma production allowed a decrease in the lag phase for γ-decalactone secretion.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/25523
url http://hdl.handle.net/1822/25523
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0003-021X
1558-9331
10.1007/s11746-013-2231-2
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
American oil chemists' society Press
publisher.none.fl_str_mv Springer
American oil chemists' society Press
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv mluisa.alvim@gmail.com
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