Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/20208 https://doi.org/10.1186/1756-9966-31-32 |
Resumo: | Background - Obesity is associated with prostate cancer aggressiveness and mortality. The contribution of periprostatic adipose tissue, which is often infiltrated by malignant cells, to cancer progression is largely unknown. Thus, this study aimed to determine if periprostatic adipose tissue is linked with aggressive tumor biology in prostate cancer. Methods - Supernatants of whole adipose tissue (explants) or stromal vascular fraction (SVF) from paired fat samples of periprostatic (PP) and pre-peritoneal visceral (VIS) anatomic origin from different donors were prepared and analyzed for matrix metalloproteinases (MMPs) 2 and 9 activity. The effects of those conditioned media (CM) on growth and migration of hormone-refractory (PC-3) and hormone-sensitive (LNCaP) prostate cancer cells were measured. Results - We show here that PP adipose tissue of overweight men has higher MMP9 activity in comparison with normal subjects. The observed increased activities of both MMP2 and MMP9 in PP whole adipose tissue explants, likely reveal the contribution of adipocytes plus stromal-vascular fraction (SVF) as opposed to SVF alone. MMP2 activity was higher for PP when compared to VIS adipose tissue. When PC-3 cells were stimulated with CM from PP adipose tissue explants, increased proliferative and migratory capacities were observed, but not in the presence of SVF. Conversely, when LNCaP cells were stimulated with PP explants CM, we found enhanced motility despite the inhibition of proliferation, whereas CM derived from SVF increased both cell proliferation and motility. Explants culture and using adipose tissue of PP origin are most effective in promoting proliferation and migration of PC-3 cells, as respectively compared with SVF culture and using adipose tissue of VIS origin. In LNCaP cells, while explants CM cause increased migration compared to SVF, the use of PP adipose tissue to generate CM result in the increase of both cellular proliferation and migration. Conclusions - Our findings suggest that the PP depot has the potential to modulate extra-prostatic tumor cells' microenvironment through increased MMPs activity and to promote prostate cancer cell survival and migration. Adipocyte-derived factors likely have a relevant proliferative and motile role. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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7160 |
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Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitroProstate cancerAdipose tissueCell lineCell proliferationCell trackingBackground - Obesity is associated with prostate cancer aggressiveness and mortality. The contribution of periprostatic adipose tissue, which is often infiltrated by malignant cells, to cancer progression is largely unknown. Thus, this study aimed to determine if periprostatic adipose tissue is linked with aggressive tumor biology in prostate cancer. Methods - Supernatants of whole adipose tissue (explants) or stromal vascular fraction (SVF) from paired fat samples of periprostatic (PP) and pre-peritoneal visceral (VIS) anatomic origin from different donors were prepared and analyzed for matrix metalloproteinases (MMPs) 2 and 9 activity. The effects of those conditioned media (CM) on growth and migration of hormone-refractory (PC-3) and hormone-sensitive (LNCaP) prostate cancer cells were measured. Results - We show here that PP adipose tissue of overweight men has higher MMP9 activity in comparison with normal subjects. The observed increased activities of both MMP2 and MMP9 in PP whole adipose tissue explants, likely reveal the contribution of adipocytes plus stromal-vascular fraction (SVF) as opposed to SVF alone. MMP2 activity was higher for PP when compared to VIS adipose tissue. When PC-3 cells were stimulated with CM from PP adipose tissue explants, increased proliferative and migratory capacities were observed, but not in the presence of SVF. Conversely, when LNCaP cells were stimulated with PP explants CM, we found enhanced motility despite the inhibition of proliferation, whereas CM derived from SVF increased both cell proliferation and motility. Explants culture and using adipose tissue of PP origin are most effective in promoting proliferation and migration of PC-3 cells, as respectively compared with SVF culture and using adipose tissue of VIS origin. In LNCaP cells, while explants CM cause increased migration compared to SVF, the use of PP adipose tissue to generate CM result in the increase of both cellular proliferation and migration. Conclusions - Our findings suggest that the PP depot has the potential to modulate extra-prostatic tumor cells' microenvironment through increased MMPs activity and to promote prostate cancer cell survival and migration. Adipocyte-derived factors likely have a relevant proliferative and motile role.BioMed Central Ltd2012-04-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/20208http://hdl.handle.net/10316/20208https://doi.org/10.1186/1756-9966-31-32engRIBEIRO, Ricardo [et. al.] - Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro. "Journal of Experimental and Clinical Cancer Research". ISSN 0392-9078. 31:1 (2012) 32http://www.jeccr.com/content/31/1/32Ribeiro, RicardoMonteiro, CátiaCunha, VirgíniaOliveira, Maria JoséFreitas, MarianaFraga, AvelinoPríncipe, PauloLobato, CarlosLobo, FranciscoMorais, AntónioSilva, VítorSanches-Magalhães, JoséOliveira, JorgePina, FranciscoMota-Pinto, AnabelaLopes, CarlosMedeiros, Ruiinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2020-08-28T13:21:57Zoai:estudogeral.uc.pt:10316/20208Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:43:38.898242Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
title |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
spellingShingle |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro Ribeiro, Ricardo Prostate cancer Adipose tissue Cell line Cell proliferation Cell tracking |
title_short |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
title_full |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
title_fullStr |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
title_full_unstemmed |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
title_sort |
Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro |
author |
Ribeiro, Ricardo |
author_facet |
Ribeiro, Ricardo Monteiro, Cátia Cunha, Virgínia Oliveira, Maria José Freitas, Mariana Fraga, Avelino Príncipe, Paulo Lobato, Carlos Lobo, Francisco Morais, António Silva, Vítor Sanches-Magalhães, José Oliveira, Jorge Pina, Francisco Mota-Pinto, Anabela Lopes, Carlos Medeiros, Rui |
author_role |
author |
author2 |
Monteiro, Cátia Cunha, Virgínia Oliveira, Maria José Freitas, Mariana Fraga, Avelino Príncipe, Paulo Lobato, Carlos Lobo, Francisco Morais, António Silva, Vítor Sanches-Magalhães, José Oliveira, Jorge Pina, Francisco Mota-Pinto, Anabela Lopes, Carlos Medeiros, Rui |
author2_role |
author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Ricardo Monteiro, Cátia Cunha, Virgínia Oliveira, Maria José Freitas, Mariana Fraga, Avelino Príncipe, Paulo Lobato, Carlos Lobo, Francisco Morais, António Silva, Vítor Sanches-Magalhães, José Oliveira, Jorge Pina, Francisco Mota-Pinto, Anabela Lopes, Carlos Medeiros, Rui |
dc.subject.por.fl_str_mv |
Prostate cancer Adipose tissue Cell line Cell proliferation Cell tracking |
topic |
Prostate cancer Adipose tissue Cell line Cell proliferation Cell tracking |
description |
Background - Obesity is associated with prostate cancer aggressiveness and mortality. The contribution of periprostatic adipose tissue, which is often infiltrated by malignant cells, to cancer progression is largely unknown. Thus, this study aimed to determine if periprostatic adipose tissue is linked with aggressive tumor biology in prostate cancer. Methods - Supernatants of whole adipose tissue (explants) or stromal vascular fraction (SVF) from paired fat samples of periprostatic (PP) and pre-peritoneal visceral (VIS) anatomic origin from different donors were prepared and analyzed for matrix metalloproteinases (MMPs) 2 and 9 activity. The effects of those conditioned media (CM) on growth and migration of hormone-refractory (PC-3) and hormone-sensitive (LNCaP) prostate cancer cells were measured. Results - We show here that PP adipose tissue of overweight men has higher MMP9 activity in comparison with normal subjects. The observed increased activities of both MMP2 and MMP9 in PP whole adipose tissue explants, likely reveal the contribution of adipocytes plus stromal-vascular fraction (SVF) as opposed to SVF alone. MMP2 activity was higher for PP when compared to VIS adipose tissue. When PC-3 cells were stimulated with CM from PP adipose tissue explants, increased proliferative and migratory capacities were observed, but not in the presence of SVF. Conversely, when LNCaP cells were stimulated with PP explants CM, we found enhanced motility despite the inhibition of proliferation, whereas CM derived from SVF increased both cell proliferation and motility. Explants culture and using adipose tissue of PP origin are most effective in promoting proliferation and migration of PC-3 cells, as respectively compared with SVF culture and using adipose tissue of VIS origin. In LNCaP cells, while explants CM cause increased migration compared to SVF, the use of PP adipose tissue to generate CM result in the increase of both cellular proliferation and migration. Conclusions - Our findings suggest that the PP depot has the potential to modulate extra-prostatic tumor cells' microenvironment through increased MMPs activity and to promote prostate cancer cell survival and migration. Adipocyte-derived factors likely have a relevant proliferative and motile role. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-02 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/20208 http://hdl.handle.net/10316/20208 https://doi.org/10.1186/1756-9966-31-32 |
url |
http://hdl.handle.net/10316/20208 https://doi.org/10.1186/1756-9966-31-32 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
RIBEIRO, Ricardo [et. al.] - Human periprostatic adipose tissue promotes prostate cancer aggressiveness in vitro. "Journal of Experimental and Clinical Cancer Research". ISSN 0392-9078. 31:1 (2012) 32 http://www.jeccr.com/content/31/1/32 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
BioMed Central Ltd |
publisher.none.fl_str_mv |
BioMed Central Ltd |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799133708532318208 |