Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect

Detalhes bibliográficos
Autor(a) principal: Mina, Diogo
Data de Publicação: 2020
Outros Autores: Pereira, Jose Alberto, Lino-Neto, T., Baptista, Paula
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/73147
Resumo: Variation on bacterial communities living in the phyllosphere as epiphytes and endophytes has been attributed to plant host effects. However, there is contradictory or inconclusive evidence regarding the effect of plant genetics (below the species' level) and of plant tissue type on phyllosphere bacterial community assembly, in particular when epiphytes and endophytes are considered simultaneously. Here, both surface and internal bacterial communities of two olive (Olea europaea) cultivars were evaluated in twigs and leaves by molecular identification of cultivable isolates, with an attempt to answer these questions. Overall,Proteobacteria,ActinobacteriaandFirmicuteswere the dominant phyla, being epiphytes more diverse and abundant than endophytes. Host genotype (at cultivar level) had a structuring effect on the composition of bacterial communities and, in a similar way, for both epiphytes and endophytes. Plant organ (leaf vs. twig) control of the bacterial communities was less evident when compared with plant genotype and with a greater influence on epiphytic than on endophytic community structure. Each olive genotype/plant organ was apparently selective towards specific bacterial operational taxonomic units (OTUs), which may lead to specific feedbacks on fitness of plant genotypes. Bacterial recruitment was observed to happen mainly within epiphytes than in endophytes and in leaves as compared with twigs. Such host specificity suggested that the benefits derived from the plant-bacteria interaction should be considered at genetic levels below the species.
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spelling Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effectOlea europaeaLMicrobiotaDiversityOrgan specificityHost specificityCiências Naturais::Ciências BiológicasScience & TechnologyVariation on bacterial communities living in the phyllosphere as epiphytes and endophytes has been attributed to plant host effects. However, there is contradictory or inconclusive evidence regarding the effect of plant genetics (below the species' level) and of plant tissue type on phyllosphere bacterial community assembly, in particular when epiphytes and endophytes are considered simultaneously. Here, both surface and internal bacterial communities of two olive (Olea europaea) cultivars were evaluated in twigs and leaves by molecular identification of cultivable isolates, with an attempt to answer these questions. Overall,Proteobacteria,ActinobacteriaandFirmicuteswere the dominant phyla, being epiphytes more diverse and abundant than endophytes. Host genotype (at cultivar level) had a structuring effect on the composition of bacterial communities and, in a similar way, for both epiphytes and endophytes. Plant organ (leaf vs. twig) control of the bacterial communities was less evident when compared with plant genotype and with a greater influence on epiphytic than on endophytic community structure. Each olive genotype/plant organ was apparently selective towards specific bacterial operational taxonomic units (OTUs), which may lead to specific feedbacks on fitness of plant genotypes. Bacterial recruitment was observed to happen mainly within epiphytes than in endophytes and in leaves as compared with twigs. Such host specificity suggested that the benefits derived from the plant-bacteria interaction should be considered at genetic levels below the species.The authors are grateful to the FEDER and Foundation for Science and Technology (FCT, Portugal) under Programme PT2020 for financial support to CIMO (UID/AGR/00690/2019) and BioISI (UID/MULTI/04046/2013), as well as the Horizon 2020, the European Union’s Framework Programme for Research and Innovation, for financial support the project PRIMA/0002/2018 INTOMED—Innovative tools to combat crop pests in the Mediterranean. D. Mina thanks FCT, POPH-QREN and FSE for SFRH-BD-105341/2014 grant.SpringerUniversidade do MinhoMina, DiogoPereira, Jose AlbertoLino-Neto, T.Baptista, Paula20202020-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/73147engMina, D., Pereira, J.A., Lino-Neto, T. et al. Epiphytic and Endophytic Bacteria on Olive Tree Phyllosphere: Exploring Tissue and Cultivar Effect. Microb Ecol 80, 145–157 (2020). https://doi.org/10.1007/s00248-020-01488-80095-362810.1007/s00248-020-01488-831965223https://link.springer.com/article/10.1007%2Fs00248-020-01488-8info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:02:35Zoai:repositorium.sdum.uminho.pt:1822/73147Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:52:35.094141Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
title Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
spellingShingle Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
Mina, Diogo
Olea europaeaL
Microbiota
Diversity
Organ specificity
Host specificity
Ciências Naturais::Ciências Biológicas
Science & Technology
title_short Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
title_full Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
title_fullStr Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
title_full_unstemmed Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
title_sort Epiphytic and endophytic bacteria on olive tree phyllosphere: Exploring tissue and cultivar effect
author Mina, Diogo
author_facet Mina, Diogo
Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
author_role author
author2 Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Mina, Diogo
Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
dc.subject.por.fl_str_mv Olea europaeaL
Microbiota
Diversity
Organ specificity
Host specificity
Ciências Naturais::Ciências Biológicas
Science & Technology
topic Olea europaeaL
Microbiota
Diversity
Organ specificity
Host specificity
Ciências Naturais::Ciências Biológicas
Science & Technology
description Variation on bacterial communities living in the phyllosphere as epiphytes and endophytes has been attributed to plant host effects. However, there is contradictory or inconclusive evidence regarding the effect of plant genetics (below the species' level) and of plant tissue type on phyllosphere bacterial community assembly, in particular when epiphytes and endophytes are considered simultaneously. Here, both surface and internal bacterial communities of two olive (Olea europaea) cultivars were evaluated in twigs and leaves by molecular identification of cultivable isolates, with an attempt to answer these questions. Overall,Proteobacteria,ActinobacteriaandFirmicuteswere the dominant phyla, being epiphytes more diverse and abundant than endophytes. Host genotype (at cultivar level) had a structuring effect on the composition of bacterial communities and, in a similar way, for both epiphytes and endophytes. Plant organ (leaf vs. twig) control of the bacterial communities was less evident when compared with plant genotype and with a greater influence on epiphytic than on endophytic community structure. Each olive genotype/plant organ was apparently selective towards specific bacterial operational taxonomic units (OTUs), which may lead to specific feedbacks on fitness of plant genotypes. Bacterial recruitment was observed to happen mainly within epiphytes than in endophytes and in leaves as compared with twigs. Such host specificity suggested that the benefits derived from the plant-bacteria interaction should be considered at genetic levels below the species.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/73147
url http://hdl.handle.net/1822/73147
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Mina, D., Pereira, J.A., Lino-Neto, T. et al. Epiphytic and Endophytic Bacteria on Olive Tree Phyllosphere: Exploring Tissue and Cultivar Effect. Microb Ecol 80, 145–157 (2020). https://doi.org/10.1007/s00248-020-01488-8
0095-3628
10.1007/s00248-020-01488-8
31965223
https://link.springer.com/article/10.1007%2Fs00248-020-01488-8
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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