KIF13A mediates influenza a virus ribonucleoproteins trafficking

Detalhes bibliográficos
Autor(a) principal: Ramos-Nascimento, Ana
Data de Publicação: 2017
Outros Autores: Kellen, Bárbara, Ferreira, Filipe, Alenquer, Marta, Vale-Costa, Silvia, Raposo, Graça, Delevoye, Cédric, Amorim, Maria João
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.7/795
Resumo: The deposited article version contains attached the supplementary materials within the pdf. This publication hasn't any creative commons license associated. The deposited article version is a "JCS Advance Online Article" provided by The Company of Biologists, and it is the "Accepted manuscript" posted online on 23 October 2017.
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spelling KIF13A mediates influenza a virus ribonucleoproteins traffickingKIF13ARecycling endosomeInfluenza A virus assemblyMolecular motorThe deposited article version contains attached the supplementary materials within the pdf. This publication hasn't any creative commons license associated. The deposited article version is a "JCS Advance Online Article" provided by The Company of Biologists, and it is the "Accepted manuscript" posted online on 23 October 2017.Influenza A is a rapid evolving virus, successful in provoking periodic epidemics and occasional pandemics in humans. Viral assembly is complex as the virus incorporates an eight-partite segmented genome of RNA (in the form of viral ribonucleoproteins, vRNPs). Genome assembly, with implications to public health, is not completely understood. It was reported that vRNPs are transported to the cell surface on Rab11 vesicles using microtubules, but no molecular motor has been assigned to the process. Here, we have identified KIF13A, a member of the kinesin-3 family, as the first molecular motor efficiently transporting vRNP-Rab11 vesicles during IAV infection. Depletion of KIF13A resulted in reduced viral titres and less accumulation of vRNPs at the cell surface, without interfering with the levels of other viral proteins at sites of viral assembly. In addition, in overexpression conditions and using two artificial methods able to displace vRNP-Rab11 vesicles, KIF13A augmented vRNP levels at the plasma membrane. Together our results show that KIF13A is an important host factor promoting influenza A vRNP transport, which is a crucial step for viral assembly.Fundação para a Ciência e a Tecnologia grants: (PTDC/IMI-MIC/1142/2012, IF/00899/2013, SFRH/BPD/62982/2009, SFRH/BPD/94204/2013); Instituto Calouste Gulbenkian; Fundação Calouste Gulbenkian; Fondation pour la Recherche Médicale grant: (Equipe FRM DEQ20140329491 Team label); Fondation ARC pour la Recherche sur le Cancer grant: (PJA20161204965); CNRS; INSERM; Institut Curie.Company of BiologistsARCARamos-Nascimento, AnaKellen, BárbaraFerreira, FilipeAlenquer, MartaVale-Costa, SilviaRaposo, GraçaDelevoye, CédricAmorim, Maria João2018-11-01T01:30:09Z2017-10-232017-10-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/795eng10.1242/jcs.210807info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:12Zoai:arca.igc.gulbenkian.pt:10400.7/795Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:01.199714Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv KIF13A mediates influenza a virus ribonucleoproteins trafficking
title KIF13A mediates influenza a virus ribonucleoproteins trafficking
spellingShingle KIF13A mediates influenza a virus ribonucleoproteins trafficking
Ramos-Nascimento, Ana
KIF13A
Recycling endosome
Influenza A virus assembly
Molecular motor
title_short KIF13A mediates influenza a virus ribonucleoproteins trafficking
title_full KIF13A mediates influenza a virus ribonucleoproteins trafficking
title_fullStr KIF13A mediates influenza a virus ribonucleoproteins trafficking
title_full_unstemmed KIF13A mediates influenza a virus ribonucleoproteins trafficking
title_sort KIF13A mediates influenza a virus ribonucleoproteins trafficking
author Ramos-Nascimento, Ana
author_facet Ramos-Nascimento, Ana
Kellen, Bárbara
Ferreira, Filipe
Alenquer, Marta
Vale-Costa, Silvia
Raposo, Graça
Delevoye, Cédric
Amorim, Maria João
author_role author
author2 Kellen, Bárbara
Ferreira, Filipe
Alenquer, Marta
Vale-Costa, Silvia
Raposo, Graça
Delevoye, Cédric
Amorim, Maria João
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv ARCA
dc.contributor.author.fl_str_mv Ramos-Nascimento, Ana
Kellen, Bárbara
Ferreira, Filipe
Alenquer, Marta
Vale-Costa, Silvia
Raposo, Graça
Delevoye, Cédric
Amorim, Maria João
dc.subject.por.fl_str_mv KIF13A
Recycling endosome
Influenza A virus assembly
Molecular motor
topic KIF13A
Recycling endosome
Influenza A virus assembly
Molecular motor
description The deposited article version contains attached the supplementary materials within the pdf. This publication hasn't any creative commons license associated. The deposited article version is a "JCS Advance Online Article" provided by The Company of Biologists, and it is the "Accepted manuscript" posted online on 23 October 2017.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-23
2017-10-23T00:00:00Z
2018-11-01T01:30:09Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.7/795
url http://hdl.handle.net/10400.7/795
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1242/jcs.210807
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Company of Biologists
publisher.none.fl_str_mv Company of Biologists
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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institution RCAAP
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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