Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2024 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/166067 |
Resumo: | Funding Information: This research was funded by Fundação para a Ciência e a Tecnologia (PTDC/MEC-MET/29314/2017, and 2022.05764.PTDC), by the European Comission CORDIS Pas Gras Project (101080329) and by the European Union EVCA Twining Project (Horizon GA n° 101079264). Publisher Copyright: © 2024 by the authors. |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesityextracellular vesiclesmetabolismobesitypost-translational modificationsprediabetesprotein acetylationprotein glycationproteomicsFood ScienceNutrition and DieteticsSDG 3 - Good Health and Well-beingFunding Information: This research was funded by Fundação para a Ciência e a Tecnologia (PTDC/MEC-MET/29314/2017, and 2022.05764.PTDC), by the European Comission CORDIS Pas Gras Project (101080329) and by the European Union EVCA Twining Project (Horizon GA n° 101079264). Publisher Copyright: © 2024 by the authors.Obesity entails metabolic alterations across multiple organs, highlighting the role of inter-organ communication in its pathogenesis. Extracellular vesicles (EVs) are communication agents in physiological and pathological conditions, and although they have been associated with obesity comorbidities, their protein cargo in this context remains largely unknown. To decipher the messages encapsulated in EVs, we isolated plasma-derived EVs from a diet-induced obese murine model. Obese plasma EVs exhibited a decline in protein diversity while control EVs revealed significant enrichment in protein-folding functions, highlighting the importance of proper folding in maintaining metabolic homeostasis. Previously, we revealed that gut-derived EVs’ proteome holds particular significance in obesity. Here, we compared plasma and gut EVs and identified four proteins exclusively present in the control state of both EVs, revealing the potential for a non-invasive assessment of gut health by analyzing blood-derived EVs. Given the relevance of post-translational modifications (PTMs), we observed a shift in chromatin-related proteins from glycation to acetylation in obese gut EVs, suggesting a regulatory mechanism targeting DNA transcription during obesity. This study provides valuable insights into novel roles of EVs and protein PTMs in the intricate mechanisms underlying obesity, shedding light on potential biomarkers and pathways for future research.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)iNOVA4Health - pólo NMSRUNBaptista Pereira, PedroTorrejón, EstefaniaFerreira, InêsCarvalho, Ana SofiaTeshima, AkikoSousa-Lima, InêsBeck, Hans ChristianCosta-Silva, BrunoMatthiesen, RuneMacedo, Maria Paulade Oliveira, Rita Machado2024-04-11T00:35:10Z2024-032024-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10362/166067eng1422-8599PURE: 86125473https://doi.org/10.3390/nu16050736info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-05-22T18:20:22Zoai:run.unl.pt:10362/166067Portal AgregadorONGhttps://www.rcaap.pt/oai/openairemluisa.alvim@gmail.comopendoar:71602024-05-22T18:20:22Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| title |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| spellingShingle |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity Baptista Pereira, Pedro extracellular vesicles metabolism obesity post-translational modifications prediabetes protein acetylation protein glycation proteomics Food Science Nutrition and Dietetics SDG 3 - Good Health and Well-being |
| title_short |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| title_full |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| title_fullStr |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| title_full_unstemmed |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| title_sort |
Proteomic Profiling of Plasma- and Gut-Derived Extracellular Vesicles in Obesity |
| author |
Baptista Pereira, Pedro |
| author_facet |
Baptista Pereira, Pedro Torrejón, Estefania Ferreira, Inês Carvalho, Ana Sofia Teshima, Akiko Sousa-Lima, Inês Beck, Hans Christian Costa-Silva, Bruno Matthiesen, Rune Macedo, Maria Paula de Oliveira, Rita Machado |
| author_role |
author |
| author2 |
Torrejón, Estefania Ferreira, Inês Carvalho, Ana Sofia Teshima, Akiko Sousa-Lima, Inês Beck, Hans Christian Costa-Silva, Bruno Matthiesen, Rune Macedo, Maria Paula de Oliveira, Rita Machado |
| author2_role |
author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) iNOVA4Health - pólo NMS RUN |
| dc.contributor.author.fl_str_mv |
Baptista Pereira, Pedro Torrejón, Estefania Ferreira, Inês Carvalho, Ana Sofia Teshima, Akiko Sousa-Lima, Inês Beck, Hans Christian Costa-Silva, Bruno Matthiesen, Rune Macedo, Maria Paula de Oliveira, Rita Machado |
| dc.subject.por.fl_str_mv |
extracellular vesicles metabolism obesity post-translational modifications prediabetes protein acetylation protein glycation proteomics Food Science Nutrition and Dietetics SDG 3 - Good Health and Well-being |
| topic |
extracellular vesicles metabolism obesity post-translational modifications prediabetes protein acetylation protein glycation proteomics Food Science Nutrition and Dietetics SDG 3 - Good Health and Well-being |
| description |
Funding Information: This research was funded by Fundação para a Ciência e a Tecnologia (PTDC/MEC-MET/29314/2017, and 2022.05764.PTDC), by the European Comission CORDIS Pas Gras Project (101080329) and by the European Union EVCA Twining Project (Horizon GA n° 101079264). Publisher Copyright: © 2024 by the authors. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-04-11T00:35:10Z 2024-03 2024-03-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/166067 |
| url |
http://hdl.handle.net/10362/166067 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
1422-8599 PURE: 86125473 https://doi.org/10.3390/nu16050736 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
| instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| instacron_str |
RCAAP |
| institution |
RCAAP |
| reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
| repository.mail.fl_str_mv |
mluisa.alvim@gmail.com |
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1817546001767989248 |