Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.5/24099 |
Resumo: | Research Areas: Infectious Diseases ; Pharmacology & Pharmacy |
id |
RCAP_a9a35b6c80ad00fb28b4366f29994a70 |
---|---|
oai_identifier_str |
oai:www.repository.utl.pt:10400.5/24099 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industryBiocideListeria monocytogenesBiofilmPlanktonic culturePulsed-field gel electrophoresisResearch Areas: Infectious Diseases ; Pharmacology & PharmacyListeria monocytogenes is a foodborne pathogen of special concern for ready-to-eat food producers. The control of its presence is a critical step in which food-grade sanitizers play an essential role. L. monocytogenes is believed to persist in food processing environments in biofilms, exhibiting less susceptibility to sanitizers than planktonic cells. This study aimed to test the susceptibility of L. monocytogenes in planktonic culture and biofilm to three commercial food-grade sanitizers and to benzalkonium chloride; together with the genetic subtyping of the isolates. L. monocytogenes isolates were collected from raw materials, final products and food-contact surfaces during a 6-year period from a ready-to-eat meat-producing food industry and genetically characterized. Serogrouping and pulsed-field gel electrophoresis (PFGE) revealed genetic variability and di erentiated L. monocytogenes isolates in three clusters. The biofilm-forming ability assay revealed that the isolates were weak biofilm producers. L. monocytogenes strains were susceptible both in the planktonic and biofilm form to oxidizing and ethanol-based compounds and to benzalkonium chloride, but not to quaternary ammonium compound. A positive association of biofilm-forming ability and LD90 values for quaternary ammonium compound and benzalkonium chloride was found. This study highlights the need for preventive measures improvement and for a conscious selection and use of sanitizers in food-related environments to control Listeria monocytogenes.MDPIRepositório da Universidade de LisboaAndrade, Joana CatarinaJoao, Antonio LopesAlonso, Carlos de SousaBARRETO, ANTONIOHenriques, Ana Rita2022-04-18T14:54:32Z2020-07-162020-07-16T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.5/24099engAndrade JC, Joao AL, Alonso CD, Barreto AS, Henriques AR. 2020. Genetic Subtyping, Biofilm-Forming Ability and Biocide Susceptibility of Listeria monocytogenes Strains Isolated from a Ready-to-Eat Food Industry, Antibiotics, 9(7):416. Doi 10.3390/antibiotics90704162079-638210.3390/antibiotics9070416info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-03-06T14:53:44Zoai:www.repository.utl.pt:10400.5/24099Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T17:08:13.256871Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
title |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
spellingShingle |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry Andrade, Joana Catarina Biocide Listeria monocytogenes Biofilm Planktonic culture Pulsed-field gel electrophoresis |
title_short |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
title_full |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
title_fullStr |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
title_full_unstemmed |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
title_sort |
Genetic subtyping, biofilm-forming ability and biocide susceptibility of Listeria monocytogenes strains isolated from a ready-to-eat food industry |
author |
Andrade, Joana Catarina |
author_facet |
Andrade, Joana Catarina Joao, Antonio Lopes Alonso, Carlos de Sousa BARRETO, ANTONIO Henriques, Ana Rita |
author_role |
author |
author2 |
Joao, Antonio Lopes Alonso, Carlos de Sousa BARRETO, ANTONIO Henriques, Ana Rita |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Repositório da Universidade de Lisboa |
dc.contributor.author.fl_str_mv |
Andrade, Joana Catarina Joao, Antonio Lopes Alonso, Carlos de Sousa BARRETO, ANTONIO Henriques, Ana Rita |
dc.subject.por.fl_str_mv |
Biocide Listeria monocytogenes Biofilm Planktonic culture Pulsed-field gel electrophoresis |
topic |
Biocide Listeria monocytogenes Biofilm Planktonic culture Pulsed-field gel electrophoresis |
description |
Research Areas: Infectious Diseases ; Pharmacology & Pharmacy |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-07-16 2020-07-16T00:00:00Z 2022-04-18T14:54:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.5/24099 |
url |
http://hdl.handle.net/10400.5/24099 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Andrade JC, Joao AL, Alonso CD, Barreto AS, Henriques AR. 2020. Genetic Subtyping, Biofilm-Forming Ability and Biocide Susceptibility of Listeria monocytogenes Strains Isolated from a Ready-to-Eat Food Industry, Antibiotics, 9(7):416. Doi 10.3390/antibiotics9070416 2079-6382 10.3390/antibiotics9070416 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799131176091254784 |