Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield

Detalhes bibliográficos
Autor(a) principal: Fonseca, Ana
Data de Publicação: 2022
Outros Autores: Conceição, Cristina, Barracosa, Paulo, Duarte, Fátima
Tipo de documento: Artigo de conferência
Idioma: por
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10174/34444
Resumo: Cynara cardunculus L. flowers have been used, in the form of an aqueous extract as vegetable coagulant, in the production of Protected Designation of Origin (PDO) ewe’s cheeses in the Mediterranean Basin. The presence of aspartic proteases, cardosins, promote specific proteolysis in milk coagulation and cheese ripening. Nine aspartic proteinases have been identified in C. cardunculus flower extracts, which have been assigned different names, cardosins A–H 1. Cardosins A and B specifically proteolyze at the surface of casein micelles in the early phases of milk coagulation. Cardosin A has a high specific proteolytic action on K-casein resulting in less softer textures, whereas cardosin B has a low proteolytic action on K-casein, and a higher non-specific proteolytic action responsible for nonfirm inner cheese texture 1. Lower ratios between specific milk clotting activity and the non-specific enzymatic proteolytic coagulant activity have been associated to lower cheese yields 2. In the present work, we evaluated the influence of the extract of flowers from two individual C. cardunculus plants (Cyn_AB, presence of cardosina A, clearly predominant, and cardosin B; Cyn_B, presence of cardosin B and absence of the typical cardosin A) on ewe cheesemaking parameters and cheese yield. The characterization of the cardosin profiles was confirmed by native-PAGE (12,5%), a technique that allows the distinction of the presence or absence of the typical cardosin A. Milk-clotting activity (MCA) was accessed by visual observation according to ISO 23058/IDF 199 (ISO/IDF, 2006). The ewe’s cheeses were manufactured in artisanal cheese manufacturer in Serpa, Portugal. Physicochemical parameters of milk and whey were evaluated, time to curd firmness (CF) was registered and the cheese yield accessed during the 30 days ripening time. The main results showed that the initial cheese yield (kg/L) per treatment differed, but there were no differences at the end of the ripening period, suggesting that the enzymatic profile of C. cardunculus flowers does affect the cheese yield.
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spelling Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yieldCynara cardunculus Lcheese yieldcardosinprorfileCynara cardunculus L. flowers have been used, in the form of an aqueous extract as vegetable coagulant, in the production of Protected Designation of Origin (PDO) ewe’s cheeses in the Mediterranean Basin. The presence of aspartic proteases, cardosins, promote specific proteolysis in milk coagulation and cheese ripening. Nine aspartic proteinases have been identified in C. cardunculus flower extracts, which have been assigned different names, cardosins A–H 1. Cardosins A and B specifically proteolyze at the surface of casein micelles in the early phases of milk coagulation. Cardosin A has a high specific proteolytic action on K-casein resulting in less softer textures, whereas cardosin B has a low proteolytic action on K-casein, and a higher non-specific proteolytic action responsible for nonfirm inner cheese texture 1. Lower ratios between specific milk clotting activity and the non-specific enzymatic proteolytic coagulant activity have been associated to lower cheese yields 2. In the present work, we evaluated the influence of the extract of flowers from two individual C. cardunculus plants (Cyn_AB, presence of cardosina A, clearly predominant, and cardosin B; Cyn_B, presence of cardosin B and absence of the typical cardosin A) on ewe cheesemaking parameters and cheese yield. The characterization of the cardosin profiles was confirmed by native-PAGE (12,5%), a technique that allows the distinction of the presence or absence of the typical cardosin A. Milk-clotting activity (MCA) was accessed by visual observation according to ISO 23058/IDF 199 (ISO/IDF, 2006). The ewe’s cheeses were manufactured in artisanal cheese manufacturer in Serpa, Portugal. Physicochemical parameters of milk and whey were evaluated, time to curd firmness (CF) was registered and the cheese yield accessed during the 30 days ripening time. The main results showed that the initial cheese yield (kg/L) per treatment differed, but there were no differences at the end of the ripening period, suggesting that the enzymatic profile of C. cardunculus flowers does affect the cheese yield.Sociedade Portuguesa de Química2023-02-15T12:20:36Z2023-02-152022-10-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjecthttp://hdl.handle.net/10174/34444http://hdl.handle.net/10174/34444porFonseca, A. R., Conceição, C., Barracosa, P., Duarte, M. F. 2022. Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield . ISBN 978-989-8124-36-4. pp249.https://bitok.datastore.pt/scimeet-prod/cms/xvieqa.events.chemistry.pt/2911d317-467d-427b-86c9-4c014b2c209d/LivroderesumosXVIEQA_2022.pdfnaonaonaondcristinaconceicao@uevora.ptndndFonseca, AnaConceição, CristinaBarracosa, PauloDuarte, Fátimainfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-03T19:36:50Zoai:dspace.uevora.pt:10174/34444Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:22:57.053722Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
title Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
spellingShingle Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
Fonseca, Ana
Cynara cardunculus L
cheese yield
cardosin
prorfile
title_short Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
title_full Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
title_fullStr Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
title_full_unstemmed Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
title_sort Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield
author Fonseca, Ana
author_facet Fonseca, Ana
Conceição, Cristina
Barracosa, Paulo
Duarte, Fátima
author_role author
author2 Conceição, Cristina
Barracosa, Paulo
Duarte, Fátima
author2_role author
author
author
dc.contributor.author.fl_str_mv Fonseca, Ana
Conceição, Cristina
Barracosa, Paulo
Duarte, Fátima
dc.subject.por.fl_str_mv Cynara cardunculus L
cheese yield
cardosin
prorfile
topic Cynara cardunculus L
cheese yield
cardosin
prorfile
description Cynara cardunculus L. flowers have been used, in the form of an aqueous extract as vegetable coagulant, in the production of Protected Designation of Origin (PDO) ewe’s cheeses in the Mediterranean Basin. The presence of aspartic proteases, cardosins, promote specific proteolysis in milk coagulation and cheese ripening. Nine aspartic proteinases have been identified in C. cardunculus flower extracts, which have been assigned different names, cardosins A–H 1. Cardosins A and B specifically proteolyze at the surface of casein micelles in the early phases of milk coagulation. Cardosin A has a high specific proteolytic action on K-casein resulting in less softer textures, whereas cardosin B has a low proteolytic action on K-casein, and a higher non-specific proteolytic action responsible for nonfirm inner cheese texture 1. Lower ratios between specific milk clotting activity and the non-specific enzymatic proteolytic coagulant activity have been associated to lower cheese yields 2. In the present work, we evaluated the influence of the extract of flowers from two individual C. cardunculus plants (Cyn_AB, presence of cardosina A, clearly predominant, and cardosin B; Cyn_B, presence of cardosin B and absence of the typical cardosin A) on ewe cheesemaking parameters and cheese yield. The characterization of the cardosin profiles was confirmed by native-PAGE (12,5%), a technique that allows the distinction of the presence or absence of the typical cardosin A. Milk-clotting activity (MCA) was accessed by visual observation according to ISO 23058/IDF 199 (ISO/IDF, 2006). The ewe’s cheeses were manufactured in artisanal cheese manufacturer in Serpa, Portugal. Physicochemical parameters of milk and whey were evaluated, time to curd firmness (CF) was registered and the cheese yield accessed during the 30 days ripening time. The main results showed that the initial cheese yield (kg/L) per treatment differed, but there were no differences at the end of the ripening period, suggesting that the enzymatic profile of C. cardunculus flowers does affect the cheese yield.
publishDate 2022
dc.date.none.fl_str_mv 2022-10-26T00:00:00Z
2023-02-15T12:20:36Z
2023-02-15
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dc.identifier.uri.fl_str_mv http://hdl.handle.net/10174/34444
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url http://hdl.handle.net/10174/34444
dc.language.iso.fl_str_mv por
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dc.relation.none.fl_str_mv Fonseca, A. R., Conceição, C., Barracosa, P., Duarte, M. F. 2022. Cynara cardunculus L. flowers enzymatic profiles and ewe’s cheese yield . ISBN 978-989-8124-36-4. pp249.
https://bitok.datastore.pt/scimeet-prod/cms/xvieqa.events.chemistry.pt/2911d317-467d-427b-86c9-4c014b2c209d/LivroderesumosXVIEQA_2022.pdf
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dc.publisher.none.fl_str_mv Sociedade Portuguesa de Química
publisher.none.fl_str_mv Sociedade Portuguesa de Química
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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