Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin

Detalhes bibliográficos
Autor(a) principal: Frazão, Bárbara
Data de Publicação: 2017
Outros Autores: Campos, Alexandre, Osório, Hugo, Thomas, Benjamin, Leandro, Sérgio, Teixeira, Alexandre, Vasconcelos, Vitor, Antunes, Agostinho
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.8/7604
Resumo: Acknowledgements BF was funded by a PhD grant (SFRH/BD/48184/2008) from the Foundation for Science and Technology “Fundação para a Ciência e a Tecnologia” (FCT). AA was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT and the European Regional Development Fund (ERDF) in the framework of the program PT2020, by the European Structural and Investment Funds (ESIF) through the Competitiveness and Internationalization Operational Program - COMPETE 2020 and by National Funds through the FCT under the projects PTDC/AAG-GLO/6887/2014 (POCI-01-0124-FEDER-016845) and PTDC/MAR-BIO/0440/2014 (POCI-01-0145-FEDER-016772), and by the Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR) and CORAL NORTE (NORTE-01-0145-FEDER-000036), funded by the Northern Regional Operational Program (NORTE2020) through the ERDF.
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spelling Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a PeroxiredoxinPelagia noctilucaJellyfishProteomicToxinAcknowledgements BF was funded by a PhD grant (SFRH/BD/48184/2008) from the Foundation for Science and Technology “Fundação para a Ciência e a Tecnologia” (FCT). AA was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT and the European Regional Development Fund (ERDF) in the framework of the program PT2020, by the European Structural and Investment Funds (ESIF) through the Competitiveness and Internationalization Operational Program - COMPETE 2020 and by National Funds through the FCT under the projects PTDC/AAG-GLO/6887/2014 (POCI-01-0124-FEDER-016845) and PTDC/MAR-BIO/0440/2014 (POCI-01-0145-FEDER-016772), and by the Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR) and CORAL NORTE (NORTE-01-0145-FEDER-000036), funded by the Northern Regional Operational Program (NORTE2020) through the ERDF.Pelagia noctiluca is the most venomous jellyfish in the Mediterranean Sea where it forms dense blooms. Although there is several published research on this species, until now none of the works has been focused on a complete protein profile of the all body constituents of this organism. Here, we have performed a detailed proteomics characterization of the major protein components expressed by P. noctiluca. With that aim, we have considered the study of jellyfish proteins involved in defense, body constituents and metabolism, and furthered explore the significance and potential application of such bioactive molecules. P. noctiluca body proteins were separated by1D SDS–PAGE and 2DE followed by characterization by nanoLC-MS/MS and MALDI-TOF/TOF techniques.Altogether, both methods revealed 68 different proteins, including a Zinc Metalloproteinase, a Red Fluorescent Protein (RFP) and a Peroxiredoxin. These three proteins were identified for the first time in P. noctiluca. Zinc Metalloproteinase was previously reported in the venom of other jellyfish species. Besides the proteins described above, the other 65 proteins found in P. noctiluca body content were identified and associated with its clinical significance. Among all the proteins identified in this work we highlight: Zinc metalloproteinase, which has a ShK toxin domain and therefore should be implicated in the sting toxicity of P. noctiluca.; the RFP which are a very important family of proteins due to its possible application as molecular markers; and last but not least the discovery of a Peroxiredoxin in this organism makes it a new natural resource of antioxidant and anti-UV radiation agents.Springer ScienceIC-OnlineFrazão, BárbaraCampos, AlexandreOsório, HugoThomas, BenjaminLeandro, SérgioTeixeira, AlexandreVasconcelos, VitorAntunes, Agostinho2022-09-06T08:27:53Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.8/7604engFrazão, B., Campos, A., Osório, H. et al. Analysis of Pelagia noctiluca proteome Reveals a Red Fluorescent Protein, a Zinc Metalloproteinase and a Peroxiredoxin. Protein J 36, 77–97 (2017). https://doi.org/10.1007/s10930-017-9695-01875-835510.1007/s10930-017-9695-0metadata only accessinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-17T15:55:32Zoai:iconline.ipleiria.pt:10400.8/7604Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:50:31.409886Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
title Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
spellingShingle Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
Frazão, Bárbara
Pelagia noctiluca
Jellyfish
Proteomic
Toxin
title_short Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
title_full Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
title_fullStr Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
title_full_unstemmed Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
title_sort Analysis of Pelagia noctiluca proteome reveals a red fluorescent protein, a zinc Metalloproteinase and a Peroxiredoxin
author Frazão, Bárbara
author_facet Frazão, Bárbara
Campos, Alexandre
Osório, Hugo
Thomas, Benjamin
Leandro, Sérgio
Teixeira, Alexandre
Vasconcelos, Vitor
Antunes, Agostinho
author_role author
author2 Campos, Alexandre
Osório, Hugo
Thomas, Benjamin
Leandro, Sérgio
Teixeira, Alexandre
Vasconcelos, Vitor
Antunes, Agostinho
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv IC-Online
dc.contributor.author.fl_str_mv Frazão, Bárbara
Campos, Alexandre
Osório, Hugo
Thomas, Benjamin
Leandro, Sérgio
Teixeira, Alexandre
Vasconcelos, Vitor
Antunes, Agostinho
dc.subject.por.fl_str_mv Pelagia noctiluca
Jellyfish
Proteomic
Toxin
topic Pelagia noctiluca
Jellyfish
Proteomic
Toxin
description Acknowledgements BF was funded by a PhD grant (SFRH/BD/48184/2008) from the Foundation for Science and Technology “Fundação para a Ciência e a Tecnologia” (FCT). AA was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT and the European Regional Development Fund (ERDF) in the framework of the program PT2020, by the European Structural and Investment Funds (ESIF) through the Competitiveness and Internationalization Operational Program - COMPETE 2020 and by National Funds through the FCT under the projects PTDC/AAG-GLO/6887/2014 (POCI-01-0124-FEDER-016845) and PTDC/MAR-BIO/0440/2014 (POCI-01-0145-FEDER-016772), and by the Structured Program of R&D&I INNOVMAR - Innovation and Sustainability in the Management and Exploitation of Marine Resources (reference NORTE-01-0145-FEDER-000035, Research Line NOVELMAR) and CORAL NORTE (NORTE-01-0145-FEDER-000036), funded by the Northern Regional Operational Program (NORTE2020) through the ERDF.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2022-09-06T08:27:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.8/7604
url http://hdl.handle.net/10400.8/7604
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Frazão, B., Campos, A., Osório, H. et al. Analysis of Pelagia noctiluca proteome Reveals a Red Fluorescent Protein, a Zinc Metalloproteinase and a Peroxiredoxin. Protein J 36, 77–97 (2017). https://doi.org/10.1007/s10930-017-9695-0
1875-8355
10.1007/s10930-017-9695-0
dc.rights.driver.fl_str_mv metadata only access
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rights_invalid_str_mv metadata only access
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Science
publisher.none.fl_str_mv Springer Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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