Isolation of a seed coagulant Moringa oleifera lectin

Detalhes bibliográficos
Autor(a) principal: Santos, Andréa F. S.
Data de Publicação: 2009
Outros Autores: Luz, Luciana A., Argôlo, Adriana C. C., Teixeira, J. A., Paiva, Patrícia M. G., Coelho, L. C. B. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/9494
Resumo: In this work hemagglutinating activity (HA) was investigated in distinct Moringa oleifera tissue extracts. A new lectin from seeds (cMoL) was purified and characterized; hemagglutinating and coagulating activities were evaluated. HA was detected in 0.15 M NaCl extracts from flowers and rachis inflorescence (5%, w/v), seeds, leaves, fundamental tissue of stem and steam bark (10%, w/v). cMoL isolated after saline extraction and guar gel column chromatography was active at pH range 4.0–9.0 agglutinating erythrocytes from rabbit and human blood types. Extracts of tissues and cMoL activities were carbohydrate inhibited; azocasein and asialofetuin abolished cMoL HA. The lectin was thermostable at 100 °C during 7 h. Polyacrylamide gel electrophoresis under reduced conditions revealed a main polypeptide band of 26.5 kDa; native basic cMoL was detected as a unique band. Seed lectin preparations and cMoL showed coagulant activity, similar to aluminium sulphate, the coagulant most widely used in water treatment.
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spelling Isolation of a seed coagulant Moringa oleifera lectinProtein purificationLectinMoringa oleiferaCoagulant activityWater treatmentNatural coagulantsScience & TechnologyIn this work hemagglutinating activity (HA) was investigated in distinct Moringa oleifera tissue extracts. A new lectin from seeds (cMoL) was purified and characterized; hemagglutinating and coagulating activities were evaluated. HA was detected in 0.15 M NaCl extracts from flowers and rachis inflorescence (5%, w/v), seeds, leaves, fundamental tissue of stem and steam bark (10%, w/v). cMoL isolated after saline extraction and guar gel column chromatography was active at pH range 4.0–9.0 agglutinating erythrocytes from rabbit and human blood types. Extracts of tissues and cMoL activities were carbohydrate inhibited; azocasein and asialofetuin abolished cMoL HA. The lectin was thermostable at 100 °C during 7 h. Polyacrylamide gel electrophoresis under reduced conditions revealed a main polypeptide band of 26.5 kDa; native basic cMoL was detected as a unique band. Seed lectin preparations and cMoL showed coagulant activity, similar to aluminium sulphate, the coagulant most widely used in water treatment.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Científico e Tecnológico (CNPq)ElsevierUniversidade do MinhoSantos, Andréa F. S.Luz, Luciana A.Argôlo, Adriana C. C.Teixeira, J. A.Paiva, Patrícia M. G.Coelho, L. C. B. B.2009-042009-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/9494eng"Process Biochemistry". ISSN 1359-5113. 44:4 (Apr. 2009) 504-508.1359-511310.1016/j.procbio.2009.01.002info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:30:07Zoai:repositorium.sdum.uminho.pt:1822/9494Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:25:14.054196Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Isolation of a seed coagulant Moringa oleifera lectin
title Isolation of a seed coagulant Moringa oleifera lectin
spellingShingle Isolation of a seed coagulant Moringa oleifera lectin
Santos, Andréa F. S.
Protein purification
Lectin
Moringa oleifera
Coagulant activity
Water treatment
Natural coagulants
Science & Technology
title_short Isolation of a seed coagulant Moringa oleifera lectin
title_full Isolation of a seed coagulant Moringa oleifera lectin
title_fullStr Isolation of a seed coagulant Moringa oleifera lectin
title_full_unstemmed Isolation of a seed coagulant Moringa oleifera lectin
title_sort Isolation of a seed coagulant Moringa oleifera lectin
author Santos, Andréa F. S.
author_facet Santos, Andréa F. S.
Luz, Luciana A.
Argôlo, Adriana C. C.
Teixeira, J. A.
Paiva, Patrícia M. G.
Coelho, L. C. B. B.
author_role author
author2 Luz, Luciana A.
Argôlo, Adriana C. C.
Teixeira, J. A.
Paiva, Patrícia M. G.
Coelho, L. C. B. B.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, Andréa F. S.
Luz, Luciana A.
Argôlo, Adriana C. C.
Teixeira, J. A.
Paiva, Patrícia M. G.
Coelho, L. C. B. B.
dc.subject.por.fl_str_mv Protein purification
Lectin
Moringa oleifera
Coagulant activity
Water treatment
Natural coagulants
Science & Technology
topic Protein purification
Lectin
Moringa oleifera
Coagulant activity
Water treatment
Natural coagulants
Science & Technology
description In this work hemagglutinating activity (HA) was investigated in distinct Moringa oleifera tissue extracts. A new lectin from seeds (cMoL) was purified and characterized; hemagglutinating and coagulating activities were evaluated. HA was detected in 0.15 M NaCl extracts from flowers and rachis inflorescence (5%, w/v), seeds, leaves, fundamental tissue of stem and steam bark (10%, w/v). cMoL isolated after saline extraction and guar gel column chromatography was active at pH range 4.0–9.0 agglutinating erythrocytes from rabbit and human blood types. Extracts of tissues and cMoL activities were carbohydrate inhibited; azocasein and asialofetuin abolished cMoL HA. The lectin was thermostable at 100 °C during 7 h. Polyacrylamide gel electrophoresis under reduced conditions revealed a main polypeptide band of 26.5 kDa; native basic cMoL was detected as a unique band. Seed lectin preparations and cMoL showed coagulant activity, similar to aluminium sulphate, the coagulant most widely used in water treatment.
publishDate 2009
dc.date.none.fl_str_mv 2009-04
2009-04-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/9494
url http://hdl.handle.net/1822/9494
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Process Biochemistry". ISSN 1359-5113. 44:4 (Apr. 2009) 504-508.
1359-5113
10.1016/j.procbio.2009.01.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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