Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice

Detalhes bibliográficos
Autor(a) principal: Vinhas, Maurícia
Data de Publicação: 2013
Outros Autores: Araújo, Ana Carolina, Ribeiro, Sónia, Rosário, Luís Brás, Belo, José António
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/34587
Resumo: © 2013 Vinhas et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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spelling Transthoracic echocardiography reference values in juvenile and adult 129/Sv miceEchocardiographyJuvenileAdult129/Sv mouseReference valuesDoppler© 2013 Vinhas et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Background: In the recent years, the use of Doppler-echocardiography has become a standard non-invasive technique in the analysis of cardiac malformations in genetically modified mice. Therefore, normal values have to be established for the most commonly used inbred strains in whose genetic background those mutations are generated. Here we provide reference values for transthoracic echocardiography measurements in juvenile (3 weeks) and adult (8 weeks) 129/Sv mice. Methods: Echocardiographic measurements were performed using B-mode, M-mode and Doppler-mode in 15 juvenile (3 weeks) and 15 adult (8 weeks) mice, during isoflurane anesthesia. M-mode measurements variability of left ventricle (LV) was determined. Results: Several echocardiographic measurements significantly differ between juvenile and adult mice. Most of these measurements are related with cardiac dimensions. All B-mode measurements were different between juveniles and adults (higher in the adults), except for fractional area change (FAC). Ejection fraction (EF) and fractional shortening (FS), calculated from M-mode parameters, do not differ between juvenile and adult mice. Stroke volume (SV) and cardiac output (CO) were significantly different between juvenile and adult mice. SV was 31.93 ± 8.67 μl in juveniles vs 70.61 ± 24.66 μl in adults, ρ < 0.001. CO was 12.06 ± 4.05 ml/min in juveniles vs 29.71 ± 10.13 ml/min in adults, ρ < 0.001. No difference was found in mitral valve (MV) and tricuspid valve (TV) related parameters between juvenile and adult mice. It was demonstrated that variability of M-mode measurements of LV is minimal. Conclusions: This study suggests that differences in cardiac dimensions, as wells as in pulmonary and aorta outflow parameters, were found between juvenile and adult mice. However, mitral and tricuspid inflow parameters seem to be similar between 3 weeks and 8 weeks mice. The reference values established in this study would contribute as a basis to future studies in post-natal cardiovascular development and diagnosing cardiovascular disorders in genetically modified mouse mutant lines.This work was supported by the Department of Biomedical Sciences and Medicine, University of Algarve and by research grants from F.C.T. and from IBB/CBME, LA to J.A. Belo. A.C. Araújo is a recipient of a FCT PhD fellowship.BioMed CentralRepositório da Universidade de LisboaVinhas, MauríciaAraújo, Ana CarolinaRibeiro, SóniaRosário, Luís BrásBelo, José António2018-08-24T09:33:08Z20132013-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/34587engCardiovascular Ultrasound 2013 11:121476-712010.1186/1476-7120-11-12info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-08T16:30:02Zoai:repositorio.ul.pt:10451/34587Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T21:49:18.802402Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
title Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
spellingShingle Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
Vinhas, Maurícia
Echocardiography
Juvenile
Adult
129/Sv mouse
Reference values
Doppler
title_short Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
title_full Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
title_fullStr Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
title_full_unstemmed Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
title_sort Transthoracic echocardiography reference values in juvenile and adult 129/Sv mice
author Vinhas, Maurícia
author_facet Vinhas, Maurícia
Araújo, Ana Carolina
Ribeiro, Sónia
Rosário, Luís Brás
Belo, José António
author_role author
author2 Araújo, Ana Carolina
Ribeiro, Sónia
Rosário, Luís Brás
Belo, José António
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Vinhas, Maurícia
Araújo, Ana Carolina
Ribeiro, Sónia
Rosário, Luís Brás
Belo, José António
dc.subject.por.fl_str_mv Echocardiography
Juvenile
Adult
129/Sv mouse
Reference values
Doppler
topic Echocardiography
Juvenile
Adult
129/Sv mouse
Reference values
Doppler
description © 2013 Vinhas et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
publishDate 2013
dc.date.none.fl_str_mv 2013
2013-01-01T00:00:00Z
2018-08-24T09:33:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/34587
url http://hdl.handle.net/10451/34587
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cardiovascular Ultrasound 2013 11:12
1476-7120
10.1186/1476-7120-11-12
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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